@inbook{4c809e2e6b3e4f5386e93ebc1737f773,
title = "Morphometric Analysis of Axons and Dendrites as a Tool for Assessing Neurotoxicity",
abstract = "Chemical perturbation of the temporal or spatial aspects of axonal or dendritic growth is associated with neurobehavioral deficits in animal models, and structural changes in axons and dendrites are thought to contribute to clinical symptoms associated with diverse neurologic diseases. Consequently, axonal and dendritic morphology are often quantified as functionally relevant endpoints of neurotoxicity. Here, we discuss methods for visualizing and quantifying axonal and dendritic morphology of neurons from the peripheral or central nervous systems in in vitro and ex vivo preparations. These methods include visualization of neuronal cytoarchitecture by immunostaining axon- or dendrite-selective antigens, transfecting cells with cDNA encoding fluorescent proteins, or labeling cells using membrane permeable small molecules that distribute throughout the cytoplasm, Golgi staining or Diolistics, as well as quantifying axonal and dendritic morphology using semi-automated or fully automated image analysis.",
keywords = "Automated image analysis, Diolistics, Golgi staining, High-content imaging, Immunocytochemistry, LUHMES cells, Neurite outgrowth, Neurotoxicity, Primary neurons, Sholl analysis",
author = "Morgan, {Rhianna K.} and Martin Schmuck and Grodzki, {Ana Cristina} and Bruun, {Donald A.} and Matelski, {Lauren E.} and Lein, {Pamela J.}",
note = "Funding Information: The authors thank Dr. Suzette Smiley-Jewell (University of California, Davis) for reviewing and editing the manuscript. This work was funded by the National Institute of Aging (R01 AG056710), the National Institute of Child Health and Human Development (P50 HD103526), and the National Institute of Environmental Health Sciences (R01 ES014901). Publisher Copyright: {\textcopyright} 2021, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2021",
doi = "10.1007/978-1-0716-1637-6_4",
language = "English (US)",
series = "Neuromethods",
publisher = "Humana Press Inc.",
pages = "51--87",
booktitle = "Neuromethods",
}