Morphologic and morphometric characterization of lung collagen content in clinically normal adult thoroughbreds in race training.

J. Lakritz, Erik R Wisner, T. Finucane, T. R. O'Brien, W. S. Tyler, John Pascoe, Charles Plopper

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Abstract

Interstitial and bronchointerstitial pulmonary patterns are commonly observed in thoracic radiographs of Thoroughbreds. Prominent interstitial and bronchointerstitial pulmonary patterns are observed in clinically normal horses, and in horses with respiratory tract disease. Until recently, the relevance of these pulmonary patterns was not known. Previous studies indicated that bronchiolitis, bronchiolar epithelial hyperplasia, epithelial metaplasia, and bronchial arteriolar recruitment correlated strongly with the prominence of the interstitial and bronchointerstitial pulmonary patterns observed radiographically. We examined the content and distribution of collagen in the lungs of 7 clinically normal Thoroughbreds in race training. After standardized fixation, lung tissue was treated with a compound that selectively stains collagen. Standard morphometric techniques were used to determine the volume density of parenchymal tissue and parenchymal airspace, mean linear intercept (estimate of alveolar size), alveolar surface area-to-volume ratio, percentage of parenchyma composed of collagen, percentage of airway wall composed of collagen, and airway wall thickness. These values were compared with radiographic and histopathologic scores obtained from the same horses. The volume density of parenchymal tissue and small airway wall thickness correlated strongly with the prominence of the bronchial and bronchointerstitial pulmonary patterns observed radiographically. Small airway thickness was also highly correlated with the perceived prominence of the interstitial pulmonary patterns observed radiographically, and morphometric estimates of parenchymal tissue and parenchymal collagen. There were also strong correlations between the volume density of parenchymal tissue, the percentage of parenchymal collagen, peribronchiolar mononuclear cell infiltrates, and bronchiolar mucosal plication estimates.(ABSTRACT TRUNCATED AT 250 WORDS)

Original languageEnglish (US)
Pages (from-to)11-18
Number of pages8
JournalAmerican Journal of Veterinary Research
Volume56
Issue number1
StatePublished - Jan 1995

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collagen
Collagen
lungs
Lung
Horses
horses
Tissue Fixation
Respiratory Tract Diseases
metaplasia
Bronchiolitis
Metaplasia
chest
hyperplasia
respiratory tract diseases
Hyperplasia
surface area
Coloring Agents
Thorax
tissues
Parenchymal Tissue

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Morphologic and morphometric characterization of lung collagen content in clinically normal adult thoroughbreds in race training. / Lakritz, J.; Wisner, Erik R; Finucane, T.; O'Brien, T. R.; Tyler, W. S.; Pascoe, John; Plopper, Charles.

In: American Journal of Veterinary Research, Vol. 56, No. 1, 01.1995, p. 11-18.

Research output: Contribution to journalArticle

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