Morphologic and cytokine profile characterization of salmonella enterica serovar typhimurium infection in calves with bovine leukocyte adhesion deficiency

J. S. Nunes, S. D. Lawhon, C. A. Rossetti, S. Khare, J. F. Figueiredo, T. Gull, R. C. Burghardt, Andreas J Baumler, Renee M Tsolis, H. L. Andrews-Polymenis, L. G. Adams

Research output: Contribution to journalArticle

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Abstract

The role of neutrophils in the pathogenesis of Salmonella enterica Typhimurium-induced ruminant and human enteritis and diarrhea has yet to be characterized with in vivo models. To address this question, the in vivo bovine ligated ileal loop model of nontyphoidal salmonellosis was used in calves with the naturally occurring bovine leukocyte adhesion deficiency (BLAD) mutation whose neutrophils are unable to extravasate and infiltrate the extravascular matrix. Data obtained from 4 BLAD Holstein calves homozygous for BLAD (CD18-), 1 to 5 weeks of age, were compared with 4 controls, age-matched Holstein calves negative for BLAD (CD18+). Morphologic studies revealed that infection of CD18- calves with S Typhimurium resulted in no significant tissue infiltration by neutrophils, less tissue damage, reduced luminal fluid accumulation, and increased bacterial invasion, when compared with CD18+ calves. Ultrastructurally, lesions in enterocytes induced by S Typhimurium infection in CD18- calves-including attachment and disruption of the brush border, apical membrane ruffling formation, and cellular degeneration-were similar to the ones reported in the literature for CD18- calves. Study of cytokine gene expression by quantitative real-time polymerase chain reaction revealed that early stages of acute infection (4-8 hours postinfection) were associated with increased interleukin 8 gene expression in the absence of tissue influx of neutrophils in CD18- calves, whereas later stages of infection (12 hours postinfection) were associated with increased expression of growth-related oncogene α in the presence of neutrophil influx in CD18+ calves. In contrast, the proinflammatory cytokines interleukin 1β and tumor necrosis factor α were poorly correlated with the presence or absence of tissue neutrophils.

Original languageEnglish (US)
Pages (from-to)322-333
Number of pages12
JournalVeterinary Pathology
Volume47
Issue number2
DOIs
StatePublished - 2010
Externally publishedYes

Fingerprint

bovine leukocyte adhesion deficiency
Salmonella enterica
Salmonella Typhimurium
Neutrophils
Leukocytes
cytokines
calves
Cytokines
neutrophils
Infection
infection
Gene Expression
Enteritis
Neutrophil Infiltration
Enterocytes
Salmonella Infections
Ruminants
Salmonella typhimurium
Microvilli
Interleukin-8

Keywords

  • Bovine leukocyte adhesion deficiency
  • Cattle
  • Chemokine
  • Intestine
  • Quantitative real-time polymerase chain reaction
  • Salmonella enterica Typhimurium
  • Scanning electron microscopy
  • Transmission electron microscopy

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Morphologic and cytokine profile characterization of salmonella enterica serovar typhimurium infection in calves with bovine leukocyte adhesion deficiency. / Nunes, J. S.; Lawhon, S. D.; Rossetti, C. A.; Khare, S.; Figueiredo, J. F.; Gull, T.; Burghardt, R. C.; Baumler, Andreas J; Tsolis, Renee M; Andrews-Polymenis, H. L.; Adams, L. G.

In: Veterinary Pathology, Vol. 47, No. 2, 2010, p. 322-333.

Research output: Contribution to journalArticle

Nunes, JS, Lawhon, SD, Rossetti, CA, Khare, S, Figueiredo, JF, Gull, T, Burghardt, RC, Baumler, AJ, Tsolis, RM, Andrews-Polymenis, HL & Adams, LG 2010, 'Morphologic and cytokine profile characterization of salmonella enterica serovar typhimurium infection in calves with bovine leukocyte adhesion deficiency', Veterinary Pathology, vol. 47, no. 2, pp. 322-333. https://doi.org/10.1177/0300985809358037
Nunes, J. S. ; Lawhon, S. D. ; Rossetti, C. A. ; Khare, S. ; Figueiredo, J. F. ; Gull, T. ; Burghardt, R. C. ; Baumler, Andreas J ; Tsolis, Renee M ; Andrews-Polymenis, H. L. ; Adams, L. G. / Morphologic and cytokine profile characterization of salmonella enterica serovar typhimurium infection in calves with bovine leukocyte adhesion deficiency. In: Veterinary Pathology. 2010 ; Vol. 47, No. 2. pp. 322-333.
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