Morphogenetic and functional activity of type II cells in early fetal rhesus monkey lungs

Ank A.W. Ten Have‐Opbroek, Charles Plopper

Research output: Contribution to journalArticle

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Abstract

To evaluate further the role of type II alveolar epithelial cells in primate lung development, lungs of fetal (46 to 155 days gestational age [DGA]), postnatal, and adult rhesus monkeys were investigated with antibodies against surfactant protein A (SP‐A), Alcian blue (AB) staining, and periodic acid‐Schiff (PAS) staining with/without alpha‐amylase pre‐treatment. In adult and postnatal lungs, type II cells (cuboid shape; large, roundish nucleus) displayed a unique cytoplasmic staining for SP‐A. In prenatal lungs, a low‐columnar to cuboid type of cell with a large, roundish nucleus was first detectable by 62 DGA. It was the only cell type to line the distalmost tubules or buds of the prospective respiratory tract. It exhibited (initially partial) cytoplasmic staining for SP‐A. AB and PAS stainings showed the presence of acid glycoconjugates and large apical and/or basal glycogen fields. After 95 DGA, the lining of the distal respiratory tract additionally displayed flatter cells with immunoreactivity for SP‐A and non‐reactive zones. Columnar epithelium (pseudostratified or simple) never stained for SP‐A. We conclude that morphologically identifiable type II cells first appear in fetal rhesus monkey lungs by 62 DGA (pseudoglandular period). The cells may already synthesize surfactant and extracellular matrix components. They generate type I cells, and thus the entire pulmonary acinus lining. These conclusions for the rhesus monkey fully agree with our earlier conclusions for another primate, the human, and for rodents. However, as presently shown, primates differ greatly from rodents with respect to the timing of type II cell differentiation (at 29–38% versus 73–75% of gestation or at 22–25% versus 48–49% of prenatal lung development). © 1992 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)93-104
Number of pages12
JournalThe Anatomical Record
Volume234
Issue number1
DOIs
StatePublished - Jan 1 1992

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Macaca mulatta
Pulmonary Surfactant-Associated Protein A
surfactants
lungs
gestational age
Lung
Gestational Age
Staining and Labeling
Primates
Alcian Blue
cells
respiratory system
proteins
Respiratory System
Rodentia
rodents
Alveolar Epithelial Cells
glycoconjugates
Glycoconjugates
Cell Shape

ASJC Scopus subject areas

  • Anatomy
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Morphogenetic and functional activity of type II cells in early fetal rhesus monkey lungs. / Ten Have‐Opbroek, Ank A.W.; Plopper, Charles.

In: The Anatomical Record, Vol. 234, No. 1, 01.01.1992, p. 93-104.

Research output: Contribution to journalArticle

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