Morphine induces gene expression of CCR5 in human CEM x174 lymphocytes

Tomoko Miyagi, Linda F. Chuang, Roy H. Doi, Maria P. Carlos, Jose V Torres, Ronald Y. Chuang

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

All HIV-1 strains studied to date use CCR5, CXCR4, or both receptors to enter cells. Simian immunodeficiency virus (SIV) infection of non-human primates has served as a useful model for understanding AIDS pathogenesis in humans. Research on several genetically divergent SIV isolates has revealed that SIV uses CCR5, and not CXCR4, for entry. CEM x174, a human lymphoid cell line, has been routinely used to cultivate and maintain various SIV strains. However, questions have arisen about how CEM x174, which reportedly was unable to express detectable amounts of CCR5 transcripts, efficiently supports the growth of SIV. In searching for an answer, we resorted to a sensitive competitive reverse transcriptase-polymerase chain reaction procedure in an attempt to detect as well as quantify the amount of CCR5 expression. Here we present our findings, which indicate that CEM x174 indeed expresses CCR5 and that the amount of CCR5 is increased in cells pretreated with morphine. These results correlate well with our previous observations that morphine treatment causes CEM x174 cells to be more susceptible to SIV infection. Similar morphine effect was not observed on CEM x174 cells infected with simian retroviruses, which do not depend on CCR5 for entry. These findings suggest a plausible mechanism whereby opiate drug users render themselves more susceptible to HIV infection, thereby explaining the vast prevalence of HIV infection among endemic drug use populations.

Original languageEnglish (US)
Pages (from-to)31305-31310
Number of pages6
JournalJournal of Biological Chemistry
Volume275
Issue number40
StatePublished - Oct 6 2000

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Simian Immunodeficiency Virus
Lymphocytes
Viruses
Gene expression
Morphine
Gene Expression
Virus Diseases
HIV Infections
Opiate Alkaloids
Simian Retroviruses
Polymerase chain reaction
RNA-Directed DNA Polymerase
Drug Users
Reverse Transcriptase Polymerase Chain Reaction
Pharmaceutical Preparations
Primates
HIV-1
Acquired Immunodeficiency Syndrome
Cells
Cell Line

ASJC Scopus subject areas

  • Biochemistry

Cite this

Miyagi, T., Chuang, L. F., Doi, R. H., Carlos, M. P., Torres, J. V., & Chuang, R. Y. (2000). Morphine induces gene expression of CCR5 in human CEM x174 lymphocytes. Journal of Biological Chemistry, 275(40), 31305-31310.

Morphine induces gene expression of CCR5 in human CEM x174 lymphocytes. / Miyagi, Tomoko; Chuang, Linda F.; Doi, Roy H.; Carlos, Maria P.; Torres, Jose V; Chuang, Ronald Y.

In: Journal of Biological Chemistry, Vol. 275, No. 40, 06.10.2000, p. 31305-31310.

Research output: Contribution to journalArticle

Miyagi, T, Chuang, LF, Doi, RH, Carlos, MP, Torres, JV & Chuang, RY 2000, 'Morphine induces gene expression of CCR5 in human CEM x174 lymphocytes', Journal of Biological Chemistry, vol. 275, no. 40, pp. 31305-31310.
Miyagi T, Chuang LF, Doi RH, Carlos MP, Torres JV, Chuang RY. Morphine induces gene expression of CCR5 in human CEM x174 lymphocytes. Journal of Biological Chemistry. 2000 Oct 6;275(40):31305-31310.
Miyagi, Tomoko ; Chuang, Linda F. ; Doi, Roy H. ; Carlos, Maria P. ; Torres, Jose V ; Chuang, Ronald Y. / Morphine induces gene expression of CCR5 in human CEM x174 lymphocytes. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 40. pp. 31305-31310.
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