Morphine enhances hepatitis C virus (HCV) replicon expression

Yuan Li, Ting Zhang, Steven D. Douglas, Jian Ping Lai, Wei Dong Xiao, David E Pleasure, Wen Zhe Ho

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Little information is available regarding whether substance abuse enhances hepatitis C virus (HCV) replication and promotes HCV disease progression. We investigated whether morphine alters HCV mRNA expression in HCV replicon-containing liver cells. Morphine significantly increased HCV mRNA expression, an effect which could be abolished by either of the opioid receptor antagonists, naltrexone or β-funaltrexamine. Investigation of the mechanism responsible for this enhancement of HCV replicon expression demonstrated that morphine activated NF-κB promoter and that caffeic acid phenethyl ester, a specific inhibitor of the activation of NF-κB, blocked morphine-activated HCV RNA expression. In addition, morphine compromised the anti-HCV effect of interferon alpha (IFN-α). Our in vitro data indicate that morphine may play an important role as a positive regulator of HCV replication in human hepatic cells and may compromise IFN-α therapy.

Original languageEnglish (US)
Pages (from-to)1167-1175
Number of pages9
JournalAmerican Journal of Pathology
Volume163
Issue number3
StatePublished - Sep 1 2003
Externally publishedYes

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Replicon
Hepacivirus
Morphine
Virus Replication
Messenger RNA
Naltrexone
Narcotic Antagonists
Virus Diseases
Interferon-alpha
Substance-Related Disorders
Disease Progression
Hepatocytes
RNA
Liver

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Li, Y., Zhang, T., Douglas, S. D., Lai, J. P., Xiao, W. D., Pleasure, D. E., & Ho, W. Z. (2003). Morphine enhances hepatitis C virus (HCV) replicon expression. American Journal of Pathology, 163(3), 1167-1175.

Morphine enhances hepatitis C virus (HCV) replicon expression. / Li, Yuan; Zhang, Ting; Douglas, Steven D.; Lai, Jian Ping; Xiao, Wei Dong; Pleasure, David E; Ho, Wen Zhe.

In: American Journal of Pathology, Vol. 163, No. 3, 01.09.2003, p. 1167-1175.

Research output: Contribution to journalArticle

Li, Y, Zhang, T, Douglas, SD, Lai, JP, Xiao, WD, Pleasure, DE & Ho, WZ 2003, 'Morphine enhances hepatitis C virus (HCV) replicon expression', American Journal of Pathology, vol. 163, no. 3, pp. 1167-1175.
Li Y, Zhang T, Douglas SD, Lai JP, Xiao WD, Pleasure DE et al. Morphine enhances hepatitis C virus (HCV) replicon expression. American Journal of Pathology. 2003 Sep 1;163(3):1167-1175.
Li, Yuan ; Zhang, Ting ; Douglas, Steven D. ; Lai, Jian Ping ; Xiao, Wei Dong ; Pleasure, David E ; Ho, Wen Zhe. / Morphine enhances hepatitis C virus (HCV) replicon expression. In: American Journal of Pathology. 2003 ; Vol. 163, No. 3. pp. 1167-1175.
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