Abstract
Little information is available regarding whether substance abuse enhances hepatitis C virus (HCV) replication and promotes HCV disease progression. We investigated whether morphine alters HCV mRNA expression in HCV replicon-containing liver cells. Morphine significantly increased HCV mRNA expression, an effect which could be abolished by either of the opioid receptor antagonists, naltrexone or β-funaltrexamine. Investigation of the mechanism responsible for this enhancement of HCV replicon expression demonstrated that morphine activated NF-κB promoter and that caffeic acid phenethyl ester, a specific inhibitor of the activation of NF-κB, blocked morphine-activated HCV RNA expression. In addition, morphine compromised the anti-HCV effect of interferon alpha (IFN-α). Our in vitro data indicate that morphine may play an important role as a positive regulator of HCV replication in human hepatic cells and may compromise IFN-α therapy.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1167-1175 |
| Number of pages | 9 |
| Journal | American Journal of Pathology |
| Volume | 163 |
| Issue number | 3 |
| State | Published - Sep 1 2003 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Pathology and Forensic Medicine
Cite this
Morphine enhances hepatitis C virus (HCV) replicon expression. / Li, Yuan; Zhang, Ting; Douglas, Steven D.; Lai, Jian Ping; Xiao, Wei Dong; Pleasure, David E; Ho, Wen Zhe.
In: American Journal of Pathology, Vol. 163, No. 3, 01.09.2003, p. 1167-1175.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Morphine enhances hepatitis C virus (HCV) replicon expression
AU - Li, Yuan
AU - Zhang, Ting
AU - Douglas, Steven D.
AU - Lai, Jian Ping
AU - Xiao, Wei Dong
AU - Pleasure, David E
AU - Ho, Wen Zhe
PY - 2003/9/1
Y1 - 2003/9/1
N2 - Little information is available regarding whether substance abuse enhances hepatitis C virus (HCV) replication and promotes HCV disease progression. We investigated whether morphine alters HCV mRNA expression in HCV replicon-containing liver cells. Morphine significantly increased HCV mRNA expression, an effect which could be abolished by either of the opioid receptor antagonists, naltrexone or β-funaltrexamine. Investigation of the mechanism responsible for this enhancement of HCV replicon expression demonstrated that morphine activated NF-κB promoter and that caffeic acid phenethyl ester, a specific inhibitor of the activation of NF-κB, blocked morphine-activated HCV RNA expression. In addition, morphine compromised the anti-HCV effect of interferon alpha (IFN-α). Our in vitro data indicate that morphine may play an important role as a positive regulator of HCV replication in human hepatic cells and may compromise IFN-α therapy.
AB - Little information is available regarding whether substance abuse enhances hepatitis C virus (HCV) replication and promotes HCV disease progression. We investigated whether morphine alters HCV mRNA expression in HCV replicon-containing liver cells. Morphine significantly increased HCV mRNA expression, an effect which could be abolished by either of the opioid receptor antagonists, naltrexone or β-funaltrexamine. Investigation of the mechanism responsible for this enhancement of HCV replicon expression demonstrated that morphine activated NF-κB promoter and that caffeic acid phenethyl ester, a specific inhibitor of the activation of NF-κB, blocked morphine-activated HCV RNA expression. In addition, morphine compromised the anti-HCV effect of interferon alpha (IFN-α). Our in vitro data indicate that morphine may play an important role as a positive regulator of HCV replication in human hepatic cells and may compromise IFN-α therapy.
UR - http://www.scopus.com/inward/record.url?scp=0042425017&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0042425017&partnerID=8YFLogxK
M3 - Article
C2 - 12937158
AN - SCOPUS:0042425017
VL - 163
SP - 1167
EP - 1175
JO - American Journal of Pathology
JF - American Journal of Pathology
SN - 0002-9440
IS - 3
ER -