Abstract
We have raised antibodies to morphine and etorphine and developed one-step enzyme-linked immunosorbent assays (ELISA) for these drugs as part of a panel of post race tests for drugs in racing horses. These tests are simple, can be completed in 2 h, and can be read by visual inspection. The morphine ELISA has an I50 for morphine of about 1.5 ng/mL, while the etorphine ELISA has an I50 for etorphine of 250 pg/mL. Cross-reactivity studies show that the antimorphine antibody cross-reacts well with levorphanol, hydromorphone, and oxycodone, while the anti-etorphine antibody showed no cross-reactivity with buprenorphine, diprenorphine, oxymorphone, morphine, or thebaine. The morphine test readily detected parent morphine or its metabolites in equine urine for at least 8 h after administration of 50 mg/horse, while a 0.1 μg/kg dose of etorphine was detectable for up to 48 h post dosing. For each test the background activity in post-race urines was equal to or less than the I50 for the standard curves, making them useful equine forensic tests. Each of these tests has detected 'positives' in post race urine samples and as such these tests are capable of substantially improving the speed and efficacy of both pre-race and post-race testing for morphine, etorphine, and their congeners in racing horses.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 305-310 |
| Number of pages | 6 |
| Journal | Journal of Analytical Toxicology |
| Volume | 15 |
| Issue number | 6 |
| State | Published - 1991 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Analytical Chemistry
- Health, Toxicology and Mutagenesis
- Toxicology
Cite this
Morphine and etorphine : XIV. Detection by ELISA in equine urine. / Stanley, Scott D; Jeganathan, A.; Wood, T.; Henry, P.; Turner, S.; Woods, W. E.; Green, M.; Tai, H. H.; Watt, D.; Blake, J.; Tobin, T.
In: Journal of Analytical Toxicology, Vol. 15, No. 6, 1991, p. 305-310.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Morphine and etorphine
T2 - XIV. Detection by ELISA in equine urine
AU - Stanley, Scott D
AU - Jeganathan, A.
AU - Wood, T.
AU - Henry, P.
AU - Turner, S.
AU - Woods, W. E.
AU - Green, M.
AU - Tai, H. H.
AU - Watt, D.
AU - Blake, J.
AU - Tobin, T.
PY - 1991
Y1 - 1991
N2 - We have raised antibodies to morphine and etorphine and developed one-step enzyme-linked immunosorbent assays (ELISA) for these drugs as part of a panel of post race tests for drugs in racing horses. These tests are simple, can be completed in 2 h, and can be read by visual inspection. The morphine ELISA has an I50 for morphine of about 1.5 ng/mL, while the etorphine ELISA has an I50 for etorphine of 250 pg/mL. Cross-reactivity studies show that the antimorphine antibody cross-reacts well with levorphanol, hydromorphone, and oxycodone, while the anti-etorphine antibody showed no cross-reactivity with buprenorphine, diprenorphine, oxymorphone, morphine, or thebaine. The morphine test readily detected parent morphine or its metabolites in equine urine for at least 8 h after administration of 50 mg/horse, while a 0.1 μg/kg dose of etorphine was detectable for up to 48 h post dosing. For each test the background activity in post-race urines was equal to or less than the I50 for the standard curves, making them useful equine forensic tests. Each of these tests has detected 'positives' in post race urine samples and as such these tests are capable of substantially improving the speed and efficacy of both pre-race and post-race testing for morphine, etorphine, and their congeners in racing horses.
AB - We have raised antibodies to morphine and etorphine and developed one-step enzyme-linked immunosorbent assays (ELISA) for these drugs as part of a panel of post race tests for drugs in racing horses. These tests are simple, can be completed in 2 h, and can be read by visual inspection. The morphine ELISA has an I50 for morphine of about 1.5 ng/mL, while the etorphine ELISA has an I50 for etorphine of 250 pg/mL. Cross-reactivity studies show that the antimorphine antibody cross-reacts well with levorphanol, hydromorphone, and oxycodone, while the anti-etorphine antibody showed no cross-reactivity with buprenorphine, diprenorphine, oxymorphone, morphine, or thebaine. The morphine test readily detected parent morphine or its metabolites in equine urine for at least 8 h after administration of 50 mg/horse, while a 0.1 μg/kg dose of etorphine was detectable for up to 48 h post dosing. For each test the background activity in post-race urines was equal to or less than the I50 for the standard curves, making them useful equine forensic tests. Each of these tests has detected 'positives' in post race urine samples and as such these tests are capable of substantially improving the speed and efficacy of both pre-race and post-race testing for morphine, etorphine, and their congeners in racing horses.
UR - http://www.scopus.com/inward/record.url?scp=0025824646&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025824646&partnerID=8YFLogxK
M3 - Article
VL - 15
SP - 305
EP - 310
JO - Journal of Analytical Toxicology
JF - Journal of Analytical Toxicology
SN - 0146-4760
IS - 6
ER -