Monovalent cations differ in their effects on transcription initiation from a σ-70 promoter of Escherichia coli

Jian Ying Wang, Karl Drlica, Michael Syvanen

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Initiation of transcription from the σ-70 rep promoter of plasmid pBR322 was measured by abortive transcription assays at various concentrations of potassium, rubidium, and sodium acetate. When linear and negatively supercoiled templates were compared, each salt generated a characteristic response. Increasing the salt concentration decreased transcription from a linear template but produced an increase (potassium) or a bell-shaped response (rubidium) with a supercoiled template. In the case of sodium ions, increasing concentration inhibited transcription initiation from both linear and supercoiled templates. These results are discussed with respect to effects of monovalent cations on DNA twist.

Original languageEnglish (US)
Pages (from-to)95-98
Number of pages4
JournalGene
Volume196
Issue number1-2
DOIs
StatePublished - Sep 1 1997

Keywords

  • DNA supercoiling
  • Potassium
  • Rubidium
  • Sodium

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Monovalent cations differ in their effects on transcription initiation from a σ-70 promoter of Escherichia coli'. Together they form a unique fingerprint.

  • Cite this