Monolithic Silica-Based Capillary Reversed-Phase Liquid Chromatography/Electrospray Mass Spectrometry for Plant Metabolomics

Vladimir V. Tolstikov, Arjen Lommen, Kazuki Nakanishi, Nobuo Tanaka, Oliver Fiehn

Research output: Contribution to journalArticle

215 Citations (Scopus)

Abstract

Application of C18 monolithic silica capillary columns in HPLC coupled to ion trap mass spectrometry detection was studied for probing the metabolome of the model plant Arabidopsis thaliana. It could be shown that the use of a long capillary column is an easy and effective approach to reduce ionization suppression by enhanced chromatographic resolution. Several hundred peaks could be detected using a 90-cm capillary column for LC separation and a noise reduction and automatic peak alignment software, which outperformed manual inspection or commercially available mass spectral deconvolution software.

Original languageEnglish (US)
Pages (from-to)6737-6740
Number of pages4
JournalAnalytical Chemistry
Volume75
Issue number23
DOIs
StatePublished - Dec 1 2003
Externally publishedYes

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Liquid chromatography
Deconvolution
Noise abatement
Silicon Dioxide
Ionization
Mass spectrometry
Inspection
Ions
Metabolomics

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Monolithic Silica-Based Capillary Reversed-Phase Liquid Chromatography/Electrospray Mass Spectrometry for Plant Metabolomics. / Tolstikov, Vladimir V.; Lommen, Arjen; Nakanishi, Kazuki; Tanaka, Nobuo; Fiehn, Oliver.

In: Analytical Chemistry, Vol. 75, No. 23, 01.12.2003, p. 6737-6740.

Research output: Contribution to journalArticle

Tolstikov, Vladimir V. ; Lommen, Arjen ; Nakanishi, Kazuki ; Tanaka, Nobuo ; Fiehn, Oliver. / Monolithic Silica-Based Capillary Reversed-Phase Liquid Chromatography/Electrospray Mass Spectrometry for Plant Metabolomics. In: Analytical Chemistry. 2003 ; Vol. 75, No. 23. pp. 6737-6740.
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