Abstract
On-chip monodisperse droplet analysis systems are ideal for low concentration and single molecule applications because they partition reagents into identical picoliter or smaller reactor volumes that can be observed in real-time. We present a novel trapping method with droplet stopping times of ∼38 ms that is applicable to most on-chip droplet generators. The technique greatly extends optical interrogation times without droplet motion or coalescence; and will allow on-chip single molecule detection of nanoparticle emitters with simple optics. The method maintains droplet monodispersity without chemistry-altering surfactants, and has been shown to preserve a stationary droplet stream through repetitive high-temperature thermal cycling with no additional energy input required to maintain droplet position.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 841-844 |
| Number of pages | 4 |
| Journal | Lab on a Chip - Miniaturisation for Chemistry and Biology |
| Volume | 9 |
| Issue number | 6 |
| DOIs | |
| State | Published - 2009 |
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ASJC Scopus subject areas
- Biochemistry
- Chemistry(all)
- Bioengineering
- Biomedical Engineering
Cite this
Monodisperse droplet generation and rapid trapping for single molecule detection and reaction kinetics measurement. / Beer, Neil Reginald; Rose, Klint Aaron; Kennedy, Ian M.
In: Lab on a Chip - Miniaturisation for Chemistry and Biology, Vol. 9, No. 6, 2009, p. 841-844.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Monodisperse droplet generation and rapid trapping for single molecule detection and reaction kinetics measurement
AU - Beer, Neil Reginald
AU - Rose, Klint Aaron
AU - Kennedy, Ian M.
PY - 2009
Y1 - 2009
N2 - On-chip monodisperse droplet analysis systems are ideal for low concentration and single molecule applications because they partition reagents into identical picoliter or smaller reactor volumes that can be observed in real-time. We present a novel trapping method with droplet stopping times of ∼38 ms that is applicable to most on-chip droplet generators. The technique greatly extends optical interrogation times without droplet motion or coalescence; and will allow on-chip single molecule detection of nanoparticle emitters with simple optics. The method maintains droplet monodispersity without chemistry-altering surfactants, and has been shown to preserve a stationary droplet stream through repetitive high-temperature thermal cycling with no additional energy input required to maintain droplet position.
AB - On-chip monodisperse droplet analysis systems are ideal for low concentration and single molecule applications because they partition reagents into identical picoliter or smaller reactor volumes that can be observed in real-time. We present a novel trapping method with droplet stopping times of ∼38 ms that is applicable to most on-chip droplet generators. The technique greatly extends optical interrogation times without droplet motion or coalescence; and will allow on-chip single molecule detection of nanoparticle emitters with simple optics. The method maintains droplet monodispersity without chemistry-altering surfactants, and has been shown to preserve a stationary droplet stream through repetitive high-temperature thermal cycling with no additional energy input required to maintain droplet position.
UR - http://www.scopus.com/inward/record.url?scp=61849115106&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=61849115106&partnerID=8YFLogxK
U2 - 10.1039/b818478j
DO - 10.1039/b818478j
M3 - Article
C2 - 19255668
AN - SCOPUS:61849115106
VL - 9
SP - 841
EP - 844
JO - Lab on a Chip - Miniaturisation for Chemistry and Biology
JF - Lab on a Chip - Miniaturisation for Chemistry and Biology
SN - 1473-0197
IS - 6
ER -