Monodisperse droplet generation and rapid trapping for single molecule detection and reaction kinetics measurement

Neil Reginald Beer, Klint Aaron Rose, Ian M. Kennedy

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

On-chip monodisperse droplet analysis systems are ideal for low concentration and single molecule applications because they partition reagents into identical picoliter or smaller reactor volumes that can be observed in real-time. We present a novel trapping method with droplet stopping times of ∼38 ms that is applicable to most on-chip droplet generators. The technique greatly extends optical interrogation times without droplet motion or coalescence; and will allow on-chip single molecule detection of nanoparticle emitters with simple optics. The method maintains droplet monodispersity without chemistry-altering surfactants, and has been shown to preserve a stationary droplet stream through repetitive high-temperature thermal cycling with no additional energy input required to maintain droplet position.

Original languageEnglish (US)
Pages (from-to)841-844
Number of pages4
JournalLab on a Chip - Miniaturisation for Chemistry and Biology
Volume9
Issue number6
DOIs
StatePublished - 2009

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)
  • Bioengineering
  • Biomedical Engineering

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