Monocyte chemotactic protein-1 (MCP-1), -2, and -3 are chemotactic for human T lymphocytes

D. D. Taub, P. Proost, William J Murphy, M. Anver, D. L. Longo, J. Van Damme, J. J. Oppenheim

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340 Scopus citations

Abstract

Monocyte chemotactic protein (MCP)-1,-2, and -3 all have been shown to induce monocyte/macrophage migration in vitro and MCP-1, also known as MCAF, chemoattracts basophils and mast cells. We report here that natural MCP-1 as well as synthetic preparations of MCP-2 and MCP-3 stimulate significant in vitro chemotaxis of human peripheral blood T lymphocytes. This MCP-induced migration was dose-dependent and directional, but not chemokinetic. Phenotypic analysis of the T cell population responsive to MCP-1, MCP-2, and MCP-3 demonstrates that both CD4+ and CD8+ T cells migrated in response to these chemokines. Similar results were observed using human CD4+ and CD8+ T cell clones. Neutralizing antisera to MCAF or MCP-2 abrogated T cell migration in response to MCP-1 and MCP-2, respectively, but not to RANTES. Subcutaneous administration of purified MCP-1 into the hind flanks of SCID mice engrafted with human peripheral blood lymphocytes (PBL) induced significant human CD3+ T cell infiltration into the site of injection at 4 h. These results demonstrate that MCP-1, MCP-2, and MCP-3 are inflammatory mediators that specifically stimulate the directional migration of T cells as well as monocytes and may play an important role in immune cell recruitment into sites of antigenic challenge.

Original languageEnglish (US)
Pages (from-to)1370-1376
Number of pages7
JournalJournal of Clinical Investigation
Volume95
Issue number3
StatePublished - 1995
Externally publishedYes

ASJC Scopus subject areas

  • Medicine(all)

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    Taub, D. D., Proost, P., Murphy, W. J., Anver, M., Longo, D. L., Van Damme, J., & Oppenheim, J. J. (1995). Monocyte chemotactic protein-1 (MCP-1), -2, and -3 are chemotactic for human T lymphocytes. Journal of Clinical Investigation, 95(3), 1370-1376.