Monoclonal antibody to a membrane glycoprotein inhibits the acrosome reaction and associated Ca2+ and H+ fluxes of sea urchin sperm

James Trimmer; Ian S. Trowbridge; Victor D. Vacquier

doi:10.1016/0092-8674(85)90218-1

Monoclonal antibody to a membrane glycoprotein inhibits the acrosome reaction and associated Ca²⁺ and H⁺ fluxes of sea urchin sperm

James Trimmer, Ian S. Trowbridge, Victor D. Vacquier

Research output: Contribution to journal › Article

77 Scopus citations

Abstract

Monoclonal antibodies were prepared using sea urchin (Strongylocentrotus purpuratus) sperm or isolated sperm plasma membrane vesicles as immunogen. Two monoclonal antibodies, J4/4 and J10/14, react with different epitopes on external domains of the same M_r = 210,000 integral-membrane glycoprotein. At Fab fragment concentrations of equivalent cell surface binding, J10/14 inhibits the egg jelly-induced exocytotic acrosome reaction and associated Ca²⁺ influx and H⁺ efflux, whereas J4/4 does not. Immunofluorescent localization shows that both monoclonal antibodies react with a narrow collar of plasma membrane over the acrosomal complex and also with the entire flagellum.

Original language	English (US)
Pages (from-to)	697-703
Number of pages	7
Journal	Cell
Volume	40
Issue number	3
DOIs	https://doi.org/10.1016/0092-8674(85)90218-1
State	Published - 1985
Externally published	Yes

Fingerprint

ASJC Scopus subject areas

Cell Biology
Molecular Biology
Medicine(all)

Cite this

Trimmer, J., Trowbridge, I. S., & Vacquier, V. D. (1985). Monoclonal antibody to a membrane glycoprotein inhibits the acrosome reaction and associated Ca²⁺ and H⁺ fluxes of sea urchin sperm. Cell, 40(3), 697-703. https://doi.org/10.1016/0092-8674(85)90218-1

@article{8613e4ef0acd4f978b4704298ea40902,

title = "Monoclonal antibody to a membrane glycoprotein inhibits the acrosome reaction and associated Ca2+ and H+ fluxes of sea urchin sperm",

abstract = "Monoclonal antibodies were prepared using sea urchin (Strongylocentrotus purpuratus) sperm or isolated sperm plasma membrane vesicles as immunogen. Two monoclonal antibodies, J4/4 and J10/14, react with different epitopes on external domains of the same Mr = 210,000 integral-membrane glycoprotein. At Fab fragment concentrations of equivalent cell surface binding, J10/14 inhibits the egg jelly-induced exocytotic acrosome reaction and associated Ca2+ influx and H+ efflux, whereas J4/4 does not. Immunofluorescent localization shows that both monoclonal antibodies react with a narrow collar of plasma membrane over the acrosomal complex and also with the entire flagellum.",

author = "James Trimmer and Trowbridge, {Ian S.} and Vacquier, {Victor D.}",

year = "1985",

doi = "10.1016/0092-8674(85)90218-1",

language = "English (US)",

volume = "40",

pages = "697--703",

journal = "Cell",

issn = "0092-8674",

publisher = "Cell Press",

number = "3",

}

TY - JOUR

T1 - Monoclonal antibody to a membrane glycoprotein inhibits the acrosome reaction and associated Ca2+ and H+ fluxes of sea urchin sperm

AU - Trimmer, James

AU - Trowbridge, Ian S.

AU - Vacquier, Victor D.

PY - 1985

Y1 - 1985

N2 - Monoclonal antibodies were prepared using sea urchin (Strongylocentrotus purpuratus) sperm or isolated sperm plasma membrane vesicles as immunogen. Two monoclonal antibodies, J4/4 and J10/14, react with different epitopes on external domains of the same Mr = 210,000 integral-membrane glycoprotein. At Fab fragment concentrations of equivalent cell surface binding, J10/14 inhibits the egg jelly-induced exocytotic acrosome reaction and associated Ca2+ influx and H+ efflux, whereas J4/4 does not. Immunofluorescent localization shows that both monoclonal antibodies react with a narrow collar of plasma membrane over the acrosomal complex and also with the entire flagellum.

AB - Monoclonal antibodies were prepared using sea urchin (Strongylocentrotus purpuratus) sperm or isolated sperm plasma membrane vesicles as immunogen. Two monoclonal antibodies, J4/4 and J10/14, react with different epitopes on external domains of the same Mr = 210,000 integral-membrane glycoprotein. At Fab fragment concentrations of equivalent cell surface binding, J10/14 inhibits the egg jelly-induced exocytotic acrosome reaction and associated Ca2+ influx and H+ efflux, whereas J4/4 does not. Immunofluorescent localization shows that both monoclonal antibodies react with a narrow collar of plasma membrane over the acrosomal complex and also with the entire flagellum.

UR - http://www.scopus.com/inward/record.url?scp=0021876563&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021876563&partnerID=8YFLogxK

U2 - 10.1016/0092-8674(85)90218-1

DO - 10.1016/0092-8674(85)90218-1

M3 - Article

C2 - 2982502

AN - SCOPUS:0021876563

VL - 40

SP - 697

EP - 703

JO - Cell

JF - Cell

SN - 0092-8674

IS - 3

ER -

Access to Document

10.1016/0092-8674(85)90218-1