Abstract
Monoclonal antibodies were prepared using sea urchin (Strongylocentrotus purpuratus) sperm or isolated sperm plasma membrane vesicles as immunogen. Two monoclonal antibodies, J4/4 and J10/14, react with different epitopes on external domains of the same Mr = 210,000 integral-membrane glycoprotein. At Fab fragment concentrations of equivalent cell surface binding, J10/14 inhibits the egg jelly-induced exocytotic acrosome reaction and associated Ca2+ influx and H+ efflux, whereas J4/4 does not. Immunofluorescent localization shows that both monoclonal antibodies react with a narrow collar of plasma membrane over the acrosomal complex and also with the entire flagellum.
Original language | English (US) |
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Pages (from-to) | 697-703 |
Number of pages | 7 |
Journal | Cell |
Volume | 40 |
Issue number | 3 |
DOIs | |
State | Published - 1985 |
ASJC Scopus subject areas
- Cell Biology
- Molecular Biology
- Medicine(all)