Monoclonal antibody to a membrane glycoprotein inhibits the acrosome reaction and associated Ca2+ and H+ fluxes of sea urchin sperm

James Trimmer; Ian S. Trowbridge; Victor D. Vacquier

doi:10.1016/0092-8674(85)90218-1

Monoclonal antibody to a membrane glycoprotein inhibits the acrosome reaction and associated Ca²⁺ and H⁺ fluxes of sea urchin sperm

James Trimmer, Ian S. Trowbridge, Victor D. Vacquier

Research output: Contribution to journal › Article

77 Citations (Scopus)

Abstract

Monoclonal antibodies were prepared using sea urchin (Strongylocentrotus purpuratus) sperm or isolated sperm plasma membrane vesicles as immunogen. Two monoclonal antibodies, J4/4 and J10/14, react with different epitopes on external domains of the same M_r = 210,000 integral-membrane glycoprotein. At Fab fragment concentrations of equivalent cell surface binding, J10/14 inhibits the egg jelly-induced exocytotic acrosome reaction and associated Ca²⁺ influx and H⁺ efflux, whereas J4/4 does not. Immunofluorescent localization shows that both monoclonal antibodies react with a narrow collar of plasma membrane over the acrosomal complex and also with the entire flagellum.

Original language	English (US)
Pages (from-to)	697-703
Number of pages	7
Journal	Cell
Volume	40
Issue number	3
DOIs	https://doi.org/10.1016/0092-8674(85)90218-1
State	Published - 1985
Externally published	Yes

Fingerprint

Acrosome Reaction

Sea Urchins

Membrane Glycoproteins

Spermatozoa

Monoclonal Antibodies

Cell membranes

Fluxes

Strongylocentrotus purpuratus

Cell Membrane

Immunoglobulin Fab Fragments

Flagella

Ovum

Epitopes

Cells

ASJC Scopus subject areas

Cell Biology
Molecular Biology
Medicine(all)

Cite this

Trimmer, J., Trowbridge, I. S., & Vacquier, V. D. (1985). Monoclonal antibody to a membrane glycoprotein inhibits the acrosome reaction and associated Ca²⁺ and H⁺ fluxes of sea urchin sperm. Cell, 40(3), 697-703. https://doi.org/10.1016/0092-8674(85)90218-1

Monoclonal antibody to a membrane glycoprotein inhibits the acrosome reaction and associated Ca²⁺ and H⁺ fluxes of sea urchin sperm. / Trimmer, James; Trowbridge, Ian S.; Vacquier, Victor D.

In: Cell, Vol. 40, No. 3, 1985, p. 697-703.

Research output: Contribution to journal › Article

Trimmer, J, Trowbridge, IS & Vacquier, VD 1985, 'Monoclonal antibody to a membrane glycoprotein inhibits the acrosome reaction and associated Ca²⁺ and H⁺ fluxes of sea urchin sperm', Cell, vol. 40, no. 3, pp. 697-703. https://doi.org/10.1016/0092-8674(85)90218-1

Trimmer J, Trowbridge IS, Vacquier VD. Monoclonal antibody to a membrane glycoprotein inhibits the acrosome reaction and associated Ca²⁺ and H⁺ fluxes of sea urchin sperm. Cell. 1985;40(3):697-703. https://doi.org/10.1016/0092-8674(85)90218-1

Trimmer, James ; Trowbridge, Ian S. ; Vacquier, Victor D. / Monoclonal antibody to a membrane glycoprotein inhibits the acrosome reaction and associated Ca²⁺ and H⁺ fluxes of sea urchin sperm. In: Cell. 1985 ; Vol. 40, No. 3. pp. 697-703.

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10.1016/0092-8674(85)90218-1