Abstract
Monoclonal antibodies were prepared using sea urchin (Strongylocentrotus purpuratus) sperm or isolated sperm plasma membrane vesicles as immunogen. Two monoclonal antibodies, J4/4 and J10/14, react with different epitopes on external domains of the same Mr = 210,000 integral-membrane glycoprotein. At Fab fragment concentrations of equivalent cell surface binding, J10/14 inhibits the egg jelly-induced exocytotic acrosome reaction and associated Ca2+ influx and H+ efflux, whereas J4/4 does not. Immunofluorescent localization shows that both monoclonal antibodies react with a narrow collar of plasma membrane over the acrosomal complex and also with the entire flagellum.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 697-703 |
| Number of pages | 7 |
| Journal | Cell |
| Volume | 40 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1985 |
ASJC Scopus subject areas
- Cell Biology
- Molecular Biology
- Medicine(all)
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Trimmer, J. S., Trowbridge, I. S., & Vacquier, V. D. (1985). Monoclonal antibody to a membrane glycoprotein inhibits the acrosome reaction and associated Ca2+ and H+ fluxes of sea urchin sperm. Cell, 40(3), 697-703. https://doi.org/10.1016/0092-8674(85)90218-1