Monoclonal antibody to a membrane glycoprotein inhibits the acrosome reaction and associated Ca2+ and H+ fluxes of sea urchin sperm

James Trimmer, Ian S. Trowbridge, Victor D. Vacquier

Research output: Contribution to journalArticle

77 Scopus citations

Abstract

Monoclonal antibodies were prepared using sea urchin (Strongylocentrotus purpuratus) sperm or isolated sperm plasma membrane vesicles as immunogen. Two monoclonal antibodies, J4/4 and J10/14, react with different epitopes on external domains of the same Mr = 210,000 integral-membrane glycoprotein. At Fab fragment concentrations of equivalent cell surface binding, J10/14 inhibits the egg jelly-induced exocytotic acrosome reaction and associated Ca2+ influx and H+ efflux, whereas J4/4 does not. Immunofluorescent localization shows that both monoclonal antibodies react with a narrow collar of plasma membrane over the acrosomal complex and also with the entire flagellum.

Original languageEnglish (US)
Pages (from-to)697-703
Number of pages7
JournalCell
Volume40
Issue number3
DOIs
StatePublished - 1985
Externally publishedYes

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ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

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