Monoclonal antibody to a membrane glycoprotein inhibits the acrosome reaction and associated Ca2+ and H+ fluxes of sea urchin sperm

James Trimmer, Ian S. Trowbridge, Victor D. Vacquier

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Monoclonal antibodies were prepared using sea urchin (Strongylocentrotus purpuratus) sperm or isolated sperm plasma membrane vesicles as immunogen. Two monoclonal antibodies, J4/4 and J10/14, react with different epitopes on external domains of the same Mr = 210,000 integral-membrane glycoprotein. At Fab fragment concentrations of equivalent cell surface binding, J10/14 inhibits the egg jelly-induced exocytotic acrosome reaction and associated Ca2+ influx and H+ efflux, whereas J4/4 does not. Immunofluorescent localization shows that both monoclonal antibodies react with a narrow collar of plasma membrane over the acrosomal complex and also with the entire flagellum.

Original languageEnglish (US)
Pages (from-to)697-703
Number of pages7
JournalCell
Volume40
Issue number3
DOIs
StatePublished - 1985
Externally publishedYes

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Acrosome Reaction
Sea Urchins
Membrane Glycoproteins
Spermatozoa
Monoclonal Antibodies
Cell membranes
Fluxes
Strongylocentrotus purpuratus
Cell Membrane
Immunoglobulin Fab Fragments
Flagella
Ovum
Epitopes
Cells

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Monoclonal antibody to a membrane glycoprotein inhibits the acrosome reaction and associated Ca2+ and H+ fluxes of sea urchin sperm. / Trimmer, James; Trowbridge, Ian S.; Vacquier, Victor D.

In: Cell, Vol. 40, No. 3, 1985, p. 697-703.

Research output: Contribution to journalArticle

Trimmer, James ; Trowbridge, Ian S. ; Vacquier, Victor D. / Monoclonal antibody to a membrane glycoprotein inhibits the acrosome reaction and associated Ca2+ and H+ fluxes of sea urchin sperm. In: Cell. 1985 ; Vol. 40, No. 3. pp. 697-703.
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