Monoclonal antibodies to three epitopic regions of feline leukemia virus p27 and their use in enzyme-linked immunosorbent assay of p27

H. Lutz, Niels C Pedersen, R. Durbin, G. H. Theilen

Research output: Contribution to journalArticle

112 Scopus citations

Abstract

Three different monoclonal antibodies were developed against the major core protein (p27) of feline leukemia virus (FeLV). Each antibody was directed against a different epitope of the species-specific portion of FeLV-p27. The 3 antibodies reacted with 5 different isolates of FeLV but not with 7 other retroviruses (MuLV (Rauscher), MuLV (AKR), MPMV, MMTV, SMRV, BAEV, RD 114). These monoclonal antibodies could readily be adapted to an enzyme-linked immunosorbent assay (ELISA) for the specific measurement of FeLV-p27. When compared in an ELISA with conventional reagents, the battery of monoclonal antibodies proved to be as sensitive as conventional polyclonal antibodies.

Original languageEnglish (US)
Pages (from-to)209-220
Number of pages12
JournalJournal of Immunological Methods
Volume56
Issue number2
DOIs
StatePublished - Jan 28 1983

Keywords

  • ELISA
  • feline leukemia virus
  • monoclonal antibodies

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

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