Monoclonal antibodies to the v-fes product and to feline leukemia: Virus P27 interspecies-specific determinants encoded by feline sarcoma viruses

Takuo Ishida, Niels C Pedersen, Gordon H. Theilen

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Monoclonal antibodies to p27 gag and v-fes specific determinants on the gag-onc polyprotein encoded by Snyder-Theilen feline sarcoma virus (ST-FeSV) were prepared. In order to obtain hybridoma clones specific to the antigenic determinants encoded by the FeSV genome, Lou rats were immunized with ST-FeSV-transformed, virus-nonproducing syngeneic cells, and boosted with either the same cells or affinity-purified feline leukemia virus (FeLV) p27. Three distinct clones reactive to both FeLV p27 and p85gag-fes, and one clone specific for a p85 fes determinant were established. The anti-p27 monoclonal antibodies also reacted with the polyproteins p959gag-fes and p839gag-fgr from Gardner-Arnstein (GA) and Theilen-Pedersen (TP1) FeSV, respectively. The anti-p27 monoclonal antibodies reacted with MuLV p30 and RD114 p28 but not with RSV, MMTV, or BLV. These results indicated that the part of the p27 gag gene that is preserved in ST-, GA, and TPI-FeSV encodes interspecies-specific p27 determinants.

Original languageEnglish (US)
Pages (from-to)678-687
Number of pages10
JournalVirology
Volume155
Issue number2
DOIs
StatePublished - 1986

Fingerprint

Feline Sarcoma Viruses
Feline Leukemia Virus
Clone Cells
Monoclonal Antibodies
Bovine Leukemia Virus
gag Genes
gag Gene Products
Polyproteins
Hybridomas
Epitopes
Genome
Viruses

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Monoclonal antibodies to the v-fes product and to feline leukemia : Virus P27 interspecies-specific determinants encoded by feline sarcoma viruses. / Ishida, Takuo; Pedersen, Niels C; Theilen, Gordon H.

In: Virology, Vol. 155, No. 2, 1986, p. 678-687.

Research output: Contribution to journalArticle

@article{d7afc870a9d74a32aebd2724840be364,
title = "Monoclonal antibodies to the v-fes product and to feline leukemia: Virus P27 interspecies-specific determinants encoded by feline sarcoma viruses",
abstract = "Monoclonal antibodies to p27 gag and v-fes specific determinants on the gag-onc polyprotein encoded by Snyder-Theilen feline sarcoma virus (ST-FeSV) were prepared. In order to obtain hybridoma clones specific to the antigenic determinants encoded by the FeSV genome, Lou rats were immunized with ST-FeSV-transformed, virus-nonproducing syngeneic cells, and boosted with either the same cells or affinity-purified feline leukemia virus (FeLV) p27. Three distinct clones reactive to both FeLV p27 and p85gag-fes, and one clone specific for a p85 fes determinant were established. The anti-p27 monoclonal antibodies also reacted with the polyproteins p959gag-fes and p839gag-fgr from Gardner-Arnstein (GA) and Theilen-Pedersen (TP1) FeSV, respectively. The anti-p27 monoclonal antibodies reacted with MuLV p30 and RD114 p28 but not with RSV, MMTV, or BLV. These results indicated that the part of the p27 gag gene that is preserved in ST-, GA, and TPI-FeSV encodes interspecies-specific p27 determinants.",
author = "Takuo Ishida and Pedersen, {Niels C} and Theilen, {Gordon H.}",
year = "1986",
doi = "10.1016/0042-6822(86)90227-8",
language = "English (US)",
volume = "155",
pages = "678--687",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Monoclonal antibodies to the v-fes product and to feline leukemia

T2 - Virus P27 interspecies-specific determinants encoded by feline sarcoma viruses

AU - Ishida, Takuo

AU - Pedersen, Niels C

AU - Theilen, Gordon H.

PY - 1986

Y1 - 1986

N2 - Monoclonal antibodies to p27 gag and v-fes specific determinants on the gag-onc polyprotein encoded by Snyder-Theilen feline sarcoma virus (ST-FeSV) were prepared. In order to obtain hybridoma clones specific to the antigenic determinants encoded by the FeSV genome, Lou rats were immunized with ST-FeSV-transformed, virus-nonproducing syngeneic cells, and boosted with either the same cells or affinity-purified feline leukemia virus (FeLV) p27. Three distinct clones reactive to both FeLV p27 and p85gag-fes, and one clone specific for a p85 fes determinant were established. The anti-p27 monoclonal antibodies also reacted with the polyproteins p959gag-fes and p839gag-fgr from Gardner-Arnstein (GA) and Theilen-Pedersen (TP1) FeSV, respectively. The anti-p27 monoclonal antibodies reacted with MuLV p30 and RD114 p28 but not with RSV, MMTV, or BLV. These results indicated that the part of the p27 gag gene that is preserved in ST-, GA, and TPI-FeSV encodes interspecies-specific p27 determinants.

AB - Monoclonal antibodies to p27 gag and v-fes specific determinants on the gag-onc polyprotein encoded by Snyder-Theilen feline sarcoma virus (ST-FeSV) were prepared. In order to obtain hybridoma clones specific to the antigenic determinants encoded by the FeSV genome, Lou rats were immunized with ST-FeSV-transformed, virus-nonproducing syngeneic cells, and boosted with either the same cells or affinity-purified feline leukemia virus (FeLV) p27. Three distinct clones reactive to both FeLV p27 and p85gag-fes, and one clone specific for a p85 fes determinant were established. The anti-p27 monoclonal antibodies also reacted with the polyproteins p959gag-fes and p839gag-fgr from Gardner-Arnstein (GA) and Theilen-Pedersen (TP1) FeSV, respectively. The anti-p27 monoclonal antibodies reacted with MuLV p30 and RD114 p28 but not with RSV, MMTV, or BLV. These results indicated that the part of the p27 gag gene that is preserved in ST-, GA, and TPI-FeSV encodes interspecies-specific p27 determinants.

UR - http://www.scopus.com/inward/record.url?scp=0022861020&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022861020&partnerID=8YFLogxK

U2 - 10.1016/0042-6822(86)90227-8

DO - 10.1016/0042-6822(86)90227-8

M3 - Article

C2 - 3024406

AN - SCOPUS:0022861020

VL - 155

SP - 678

EP - 687

JO - Virology

JF - Virology

SN - 0042-6822

IS - 2

ER -