Abstract
Monoclonal antibodies to p27 gag and v-fes specific determinants on the gag-onc polyprotein encoded by Snyder-Theilen feline sarcoma virus (ST-FeSV) were prepared. In order to obtain hybridoma clones specific to the antigenic determinants encoded by the FeSV genome, Lou rats were immunized with ST-FeSV-transformed, virus-nonproducing syngeneic cells, and boosted with either the same cells or affinity-purified feline leukemia virus (FeLV) p27. Three distinct clones reactive to both FeLV p27 and p85gag-fes, and one clone specific for a p85 fes determinant were established. The anti-p27 monoclonal antibodies also reacted with the polyproteins p959gag-fes and p839gag-fgr from Gardner-Arnstein (GA) and Theilen-Pedersen (TP1) FeSV, respectively. The anti-p27 monoclonal antibodies reacted with MuLV p30 and RD114 p28 but not with RSV, MMTV, or BLV. These results indicated that the part of the p27 gag gene that is preserved in ST-, GA, and TPI-FeSV encodes interspecies-specific p27 determinants.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 678-687 |
| Number of pages | 10 |
| Journal | Virology |
| Volume | 155 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1986 |
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ASJC Scopus subject areas
- Virology
- Infectious Diseases
Cite this
Monoclonal antibodies to the v-fes product and to feline leukemia : Virus P27 interspecies-specific determinants encoded by feline sarcoma viruses. / Ishida, Takuo; Pedersen, Niels C; Theilen, Gordon H.
In: Virology, Vol. 155, No. 2, 1986, p. 678-687.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Monoclonal antibodies to the v-fes product and to feline leukemia
T2 - Virus P27 interspecies-specific determinants encoded by feline sarcoma viruses
AU - Ishida, Takuo
AU - Pedersen, Niels C
AU - Theilen, Gordon H.
PY - 1986
Y1 - 1986
N2 - Monoclonal antibodies to p27 gag and v-fes specific determinants on the gag-onc polyprotein encoded by Snyder-Theilen feline sarcoma virus (ST-FeSV) were prepared. In order to obtain hybridoma clones specific to the antigenic determinants encoded by the FeSV genome, Lou rats were immunized with ST-FeSV-transformed, virus-nonproducing syngeneic cells, and boosted with either the same cells or affinity-purified feline leukemia virus (FeLV) p27. Three distinct clones reactive to both FeLV p27 and p85gag-fes, and one clone specific for a p85 fes determinant were established. The anti-p27 monoclonal antibodies also reacted with the polyproteins p959gag-fes and p839gag-fgr from Gardner-Arnstein (GA) and Theilen-Pedersen (TP1) FeSV, respectively. The anti-p27 monoclonal antibodies reacted with MuLV p30 and RD114 p28 but not with RSV, MMTV, or BLV. These results indicated that the part of the p27 gag gene that is preserved in ST-, GA, and TPI-FeSV encodes interspecies-specific p27 determinants.
AB - Monoclonal antibodies to p27 gag and v-fes specific determinants on the gag-onc polyprotein encoded by Snyder-Theilen feline sarcoma virus (ST-FeSV) were prepared. In order to obtain hybridoma clones specific to the antigenic determinants encoded by the FeSV genome, Lou rats were immunized with ST-FeSV-transformed, virus-nonproducing syngeneic cells, and boosted with either the same cells or affinity-purified feline leukemia virus (FeLV) p27. Three distinct clones reactive to both FeLV p27 and p85gag-fes, and one clone specific for a p85 fes determinant were established. The anti-p27 monoclonal antibodies also reacted with the polyproteins p959gag-fes and p839gag-fgr from Gardner-Arnstein (GA) and Theilen-Pedersen (TP1) FeSV, respectively. The anti-p27 monoclonal antibodies reacted with MuLV p30 and RD114 p28 but not with RSV, MMTV, or BLV. These results indicated that the part of the p27 gag gene that is preserved in ST-, GA, and TPI-FeSV encodes interspecies-specific p27 determinants.
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UR - http://www.scopus.com/inward/citedby.url?scp=0022861020&partnerID=8YFLogxK
U2 - 10.1016/0042-6822(86)90227-8
DO - 10.1016/0042-6822(86)90227-8
M3 - Article
C2 - 3024406
AN - SCOPUS:0022861020
VL - 155
SP - 678
EP - 687
JO - Virology
JF - Virology
SN - 0042-6822
IS - 2
ER -