Monoclonal antibodies to lymphocyte surface antigens for cetacean homologues to CD2, CD19 and CD21

Sylvain De Guise, Karen Erickson, Myra Blanchard, Lisa DiMolfetto, Heather D. Lepper, Janice Wang, Jeffrey L Stott, David A. Ferrick

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

CD2 is a pan-T cell marker, while CD19 and CD21 are important molecules in signal transduction of B lymphocytes. CD19 and CD21 are both present on mature B cells, while CD19 is also present in developing B cells and plasma cells. Monoclonal antibodies (mAbs) against cetacean lymphocyte putative homologues to CD2 (two different antibodies), CD19 and CD21 were characterized. The proteins immunoprecipitated were as follows: F21.I (putative anti-CD2), 43 and 59kDa; F21.B (putative anti-CD19), 83 and 127kDa; F21.F (putative anti-CD21), 144kDa. The second putative anti-CD2 (F21.C) selectively inhibited the binding of F21.I. Both the putative anti-CD2 (T cell markers) stained T-cell zones on lymph node sections, while both the B cell markers (putative CD19 and CD21) stained B-cell zones. F21.B and F21.F were absent from thymus single cell suspension but labeled 63 and 65% mesenteric lymph node lymphocytes, respectively, while both F21.C and F21.F were present on 100% thymocytes and fewer lymph node lymphocytes. B and T cell markers were mutually exclusive on double labeling using flow cytometry. These mAbs are foreseen as possible valuable diagnostic and research tools to assess immune functions of captive and wild cetaceans.

Original languageEnglish (US)
Pages (from-to)209-221
Number of pages13
JournalVeterinary Immunology and Immunopathology
Volume84
Issue number3-4
DOIs
StatePublished - 2002

Keywords

  • CD19
  • CD2
  • CD21
  • Cetaceans
  • Immunology
  • Phenotyping

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology
  • veterinary(all)

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