Monoclonal antibodies to a membrane glycoprotein induce the phosphorylation of histone H1 in sea urchin spermatozoa

V. D. vacquier, G. W. Moy, James Trimmer, Y. Ebina, D. C. Porter

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Two groups of mAbs reacting with external domains of a major sea urchin sperm membrane glycoprotein of 210 kD were isolated. Previous studies have shown that group I mAbs inhibit the acrosome reaction induced by egg jelly and also cause large increases in intracellular Ca2+ ([Ca2+](i)). Group II mAbs, at comparable levels of cell surface binding, neither inhibit the egg jelly-induced acrosome reaction nor cause increases in [Ca2+](i). In this paper, we investigate the ability of these mAbs to induce the cAMP-dependent phosphorylation of sperm histone H1. Group I mAbs induce H1 phosphorylation to the same level and on the same peptide, as occurs upon treatment of sperm with egg jelly. These mAbs also activate adenylate cyclase to the same extent as egg jelly. Group II mAbs do not induce H1 phosphorylation and are only poor activators of adenylate cyclase. Group I mAbs compete with each other, but not with group II mAbs, for binding to the cell surface. These data indicate that the activation of adenylate cyclase is an initial event in the pathway leading from the binding of mAbs to a specific domain of the 210-kD protein at the cell surface, to the discrete phosphorylation of histone H1 in highly condensed sperm chromatin. The domain on the 210-kD protein recognized by group I mAbs plays a critical role in signal transduction during the early events of fertilization.

Original languageEnglish (US)
Pages (from-to)2021-2027
Number of pages7
JournalJournal of Cell Biology
Volume107
Issue number6 I
StatePublished - 1988
Externally publishedYes

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Sea Urchins
Membrane Glycoproteins
Histones
Ovum
Spermatozoa
Monoclonal Antibodies
Phosphorylation
Adenylyl Cyclases
Acrosome Reaction
Fertilization
Chromatin
Signal Transduction
Membrane Proteins
Peptides
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

vacquier, V. D., Moy, G. W., Trimmer, J., Ebina, Y., & Porter, D. C. (1988). Monoclonal antibodies to a membrane glycoprotein induce the phosphorylation of histone H1 in sea urchin spermatozoa. Journal of Cell Biology, 107(6 I), 2021-2027.

Monoclonal antibodies to a membrane glycoprotein induce the phosphorylation of histone H1 in sea urchin spermatozoa. / vacquier, V. D.; Moy, G. W.; Trimmer, James; Ebina, Y.; Porter, D. C.

In: Journal of Cell Biology, Vol. 107, No. 6 I, 1988, p. 2021-2027.

Research output: Contribution to journalArticle

vacquier, VD, Moy, GW, Trimmer, J, Ebina, Y & Porter, DC 1988, 'Monoclonal antibodies to a membrane glycoprotein induce the phosphorylation of histone H1 in sea urchin spermatozoa', Journal of Cell Biology, vol. 107, no. 6 I, pp. 2021-2027.
vacquier, V. D. ; Moy, G. W. ; Trimmer, James ; Ebina, Y. ; Porter, D. C. / Monoclonal antibodies to a membrane glycoprotein induce the phosphorylation of histone H1 in sea urchin spermatozoa. In: Journal of Cell Biology. 1988 ; Vol. 107, No. 6 I. pp. 2021-2027.
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