Monoclonal antibodies induce the translocation, patching, and shedding of surface antigens of sea urchin spermatozoa

James Trimmer, Victor D. Vacquier

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Monoclonal antibodies reacting with external domains of plasma membrane proteins of sea urchin spermatozoa cause the reversible aggregation of sperm. The rate of disaggregation is temperature dependent indicating the involvement of membrane fluidity. Immunofluorescence shows that disaggregation is temporally correlated with the movement of surface-bound antibody into large aggregates which are then shed from the cell surface. Electrophoretic analysis of the shed antigens shows that they resemble the total complement of proteins of isolated sperm membranes. The translocation and shedding of surface-bound ligands may be a common property of eukaryotic flagellar membranes.

Original languageEnglish (US)
Pages (from-to)37-51
Number of pages15
JournalExperimental Cell Research
Volume175
Issue number1
DOIs
StatePublished - 1988
Externally publishedYes

Fingerprint

Sea Urchins
Surface Antigens
Spermatozoa
Monoclonal Antibodies
Membrane Fluidity
Membranes
Fluorescent Antibody Technique
Blood Proteins
Complement System Proteins
Membrane Proteins
Cell Membrane
Ligands
Antigens
Temperature
Antibodies

ASJC Scopus subject areas

  • Cell Biology

Cite this

Monoclonal antibodies induce the translocation, patching, and shedding of surface antigens of sea urchin spermatozoa. / Trimmer, James; Vacquier, Victor D.

In: Experimental Cell Research, Vol. 175, No. 1, 1988, p. 37-51.

Research output: Contribution to journalArticle

@article{f4e1611ee59f4927a23730bf287b6103,
title = "Monoclonal antibodies induce the translocation, patching, and shedding of surface antigens of sea urchin spermatozoa",
abstract = "Monoclonal antibodies reacting with external domains of plasma membrane proteins of sea urchin spermatozoa cause the reversible aggregation of sperm. The rate of disaggregation is temperature dependent indicating the involvement of membrane fluidity. Immunofluorescence shows that disaggregation is temporally correlated with the movement of surface-bound antibody into large aggregates which are then shed from the cell surface. Electrophoretic analysis of the shed antigens shows that they resemble the total complement of proteins of isolated sperm membranes. The translocation and shedding of surface-bound ligands may be a common property of eukaryotic flagellar membranes.",
author = "James Trimmer and Vacquier, {Victor D.}",
year = "1988",
doi = "10.1016/0014-4827(88)90253-4",
language = "English (US)",
volume = "175",
pages = "37--51",
journal = "Experimental Cell Research",
issn = "0014-4827",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Monoclonal antibodies induce the translocation, patching, and shedding of surface antigens of sea urchin spermatozoa

AU - Trimmer, James

AU - Vacquier, Victor D.

PY - 1988

Y1 - 1988

N2 - Monoclonal antibodies reacting with external domains of plasma membrane proteins of sea urchin spermatozoa cause the reversible aggregation of sperm. The rate of disaggregation is temperature dependent indicating the involvement of membrane fluidity. Immunofluorescence shows that disaggregation is temporally correlated with the movement of surface-bound antibody into large aggregates which are then shed from the cell surface. Electrophoretic analysis of the shed antigens shows that they resemble the total complement of proteins of isolated sperm membranes. The translocation and shedding of surface-bound ligands may be a common property of eukaryotic flagellar membranes.

AB - Monoclonal antibodies reacting with external domains of plasma membrane proteins of sea urchin spermatozoa cause the reversible aggregation of sperm. The rate of disaggregation is temperature dependent indicating the involvement of membrane fluidity. Immunofluorescence shows that disaggregation is temporally correlated with the movement of surface-bound antibody into large aggregates which are then shed from the cell surface. Electrophoretic analysis of the shed antigens shows that they resemble the total complement of proteins of isolated sperm membranes. The translocation and shedding of surface-bound ligands may be a common property of eukaryotic flagellar membranes.

UR - http://www.scopus.com/inward/record.url?scp=0023818959&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023818959&partnerID=8YFLogxK

U2 - 10.1016/0014-4827(88)90253-4

DO - 10.1016/0014-4827(88)90253-4

M3 - Article

VL - 175

SP - 37

EP - 51

JO - Experimental Cell Research

JF - Experimental Cell Research

SN - 0014-4827

IS - 1

ER -