Monoclonal antibodies as reversible equilibrium carriers of radiopharmaceuticals

D. A. Goodwin, C. F. Meares, G. F. David, M. McTigue, M. J. McCall, J. M. Frincke, M. R. Stone, R. M. Bartholomew, J. P. Leung

Research output: Contribution to journalArticle

Abstract

We have prepared monoclonal antibodies (MoAbs) with the specific ability to bind metal chelates such as 111In benzyl EDTA. One, 10, 50 and 100 μg MoAb CHA255 Kb = 4 × 10E9 was complexed with 111In BLEDTA II, BLEDTA IV, and benzyl EDTA and injected i.v. in Balb/c mice with KHJJ tumor. The biological half-life by whole body counting was profoundly altered for all three compounds; from minutes to hours with 10 μg; to days with 100 μg. Tumor uptake increased 50 fold at 24 h with increasing MoAb but satisfactory tumor concentrations (3% per g) and tumor/blood ratios (1.8:1) were obtained with an amount equivalent to 7 mg for a human. Blood level and whole body activity were decreased 30-50% within 3 h or i.v. injection of a "flushing" dose of unlabeled indium benzyl EDTA, increasing tumor/blood ratios to 50:1.

Original languageEnglish (US)
Pages (from-to)383-391
Number of pages9
JournalInternational Journal of Radiation Applications and Instrumentation.
Volume13
Issue number4
DOIs
StatePublished - 1986

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    Goodwin, D. A., Meares, C. F., David, G. F., McTigue, M., McCall, M. J., Frincke, J. M., Stone, M. R., Bartholomew, R. M., & Leung, J. P. (1986). Monoclonal antibodies as reversible equilibrium carriers of radiopharmaceuticals. International Journal of Radiation Applications and Instrumentation., 13(4), 383-391. https://doi.org/10.1016/0883-2897(86)90015-2