Monoclonal antibodies against purified nicotinic acetylcholine receptor

Christopher M. Gomez, David P Richman, Phillip W. Berman, Steven A. Burres, Barry G W Arnason, Frank W. Fitch

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Eleven stable monoclonal hybridoma cell lines synthesizing antibodies against purified acetylcholine receptor from Torpedo californica were produced. Spleen cells from a rat immunized with acetylcholine receptor and exhibiting experimental autoimmune myasthenia gravis were fused with a mouse myeloma cell line. Studies of the binding of these antibodies to acetylcholine receptor by use of a passive hemagglutination assay in the presence of various cholinergic ligands revealed four general categories of binding specificities: 1) blockade only by alpha bungarotoxin, 2) partial blockade by all ligands, 3) increased titer in the presence of alpha bungarotoxin and benzoquinonium chloride, 4) absence of effect by any ligand.

Original languageEnglish (US)
Pages (from-to)575-582
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume88
Issue number2
DOIs
StatePublished - May 28 1979
Externally publishedYes

Fingerprint

Nicotinic Receptors
Cholinergic Receptors
Bungarotoxins
Monoclonal Antibodies
Ligands
Autoimmune Experimental Myasthenia Gravis
Cells
Torpedo
Cell Line
Antibodies
Hybridomas
Hemagglutination
Cholinergic Agents
Rats
Chlorides
Assays
Spleen

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Monoclonal antibodies against purified nicotinic acetylcholine receptor. / Gomez, Christopher M.; Richman, David P; Berman, Phillip W.; Burres, Steven A.; Arnason, Barry G W; Fitch, Frank W.

In: Biochemical and Biophysical Research Communications, Vol. 88, No. 2, 28.05.1979, p. 575-582.

Research output: Contribution to journalArticle

Gomez, Christopher M. ; Richman, David P ; Berman, Phillip W. ; Burres, Steven A. ; Arnason, Barry G W ; Fitch, Frank W. / Monoclonal antibodies against purified nicotinic acetylcholine receptor. In: Biochemical and Biophysical Research Communications. 1979 ; Vol. 88, No. 2. pp. 575-582.
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