Monoclonal anti-endothelial cell antibodies from a patient with Takayasu arteritis activate endothelial cells from large vessels

Miri Blank, Ilan Krause, Tzipora Goldkorn, Sonja Praprotnik, Avi Livneh, Pnina Langevitz, Ella Kaganovsky, Sara Morgenstern, Shlomo Cohen, Vivian Barak, Amiram Eldor, Babette Weksler, Yehuda Shoenfeld

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65 Scopus citations

Abstract

Objective. To create monoclonal anti-endothelial cell antibodies (mAECA) from a patient with Takayasu arteritis to evaluate their ability to activate human umbilical vein endothelial cells (HUVEC), and to characterize the mechanism of EC activation. Methods. A panel of mAECA was generated from peripheral blood lymphocytes of a patient with Takayasu arteritis, using Epstein-Barr virus transformation. Activity against macrovascular EC (HUVEC) and microvascular EC (human bone marrow EC immortalized by SV40) antigens was detected by enzyme-linked immunosorbent assay. Inhibition studies were used to select the monoclonal antibodies (mAECA) which share the same EC epitope binding specificity as the total IgG-AECA from the Takayasu arteritis patient. The binding of the mAECA to human aortic EC was studied by immunohistochemistry. The secretion levels of interleukin-6 (IL-6) and von Willebrand factor (vWF) were determined, to serve as markers for EC activation. The activated EC were examined for the adherence of a monocytic cell line (U937), as well as for expression of vascular cell adhesion molecule 1, intercellular adhesion molecule 1, and E-selectin. In addition, nuclear extracts of the mAECA-treated EC were analyzed for the induction of translocation of nuclear factor κB (NF-κB), using a specific NF-κB oligoprobe in an electrophoretic mobility shift assay. Results. Six mAECA were selected, the mixture of which produced 100% inhibition of binding of the original IgG (from the patient with Takayasu arteritis) to HUVEC. All mAECA possessed high activity against macrovascular EC, but none had significant antimicrovascular EC activity. The mAECA, but not normal human IgG, had anti-human aortic EC activity. Four of the 6 mAECA activated EC, manifested by increased IL-6 and vWF secretion. The 4 mAECA induced EC expression of adhesion molecules and increased adhesion of U937 monocytic cells to EC. In addition, these mAECA stimulated the nuclear translocation of the NF-κB transcription factor. Conclusion. Our findings suggest that AECA may directly stimulate EC in Takayasu arteritis through elevation of adhesion molecule expression associated with NF-κB activation and adhesion of monocytes, and may therefore play a pathogenic role in the development of the vasculopathy in Takayasu arteritis.

Original languageEnglish (US)
Pages (from-to)1421-1432
Number of pages12
JournalArthritis and Rheumatism
Volume42
Issue number7
DOIs
StatePublished - Jul 1999
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology
  • Rheumatology

Cite this

Blank, M., Krause, I., Goldkorn, T., Praprotnik, S., Livneh, A., Langevitz, P., Kaganovsky, E., Morgenstern, S., Cohen, S., Barak, V., Eldor, A., Weksler, B., & Shoenfeld, Y. (1999). Monoclonal anti-endothelial cell antibodies from a patient with Takayasu arteritis activate endothelial cells from large vessels. Arthritis and Rheumatism, 42(7), 1421-1432. https://doi.org/10.1002/1529-0131(199907)42:7<1421::AID-ANR16>3.0.CO;2-O