Monensin concentrations measured in feeder cattle using enzyme immunoassay

M. E. Mount, James S Cullor, Philip H Kass, W. Garret

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Abstract

Thirty heifers were fed a ration containing 30 g monensin/ton. Fecal, urinary and seral samples were collected at varying intervals prior to and after initiating administration of the monensin-containing feed, and monensin concentrations were determined using a modified indirect enzyme immunoassay. Fecal samples contained measurable (μg/g; ppm) concentrations of monensin in most samples. The majority of sera and urine samples contained monensin at ng/ml (ppb) concentrations, which were above background levels prior to monensin feeding. Twelve head were fed monensin at 60 g/ton and 90 g/ton for 5 d with collection of similar samples. Higher concentrations of monensin were detected with increasing ration amounts in all 3 sample types.Enzyme immunoassay for monensin in these biological samples identified presence of the feed additive.

Original languageEnglish (US)
Pages (from-to)169-172
Number of pages4
JournalVeterinary and Human Toxicology
Volume38
Issue number3
StatePublished - Jun 1996
Externally publishedYes

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ASJC Scopus subject areas

  • veterinary(all)
  • Toxicology
  • Health, Toxicology and Mutagenesis

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