MoNa – A Cost-Efficient, Portable System for the Nanoinjection of Living Cells

Matthias Simonis, Alice Sandmeyer, Johannes Greiner, Barbara Kaltschmidt, Thomas R Huser, Simon Hennig

Research output: Contribution to journalArticle

Abstract

Injection techniques to deliver macromolecules to cells such as microinjection have been around for decades with applications ranging from probing whole organisms to the injection of fluorescent molecules into single cells. A similar technique that has raised recent interest is nanoinjection. The pipettes used here are much smaller and allow for the precise deposition of molecules into single cells via electrokinetics with minimal influence on the cells’ health. Unfortunately, the equipment utilized for nanoinjection originates from scanning ion conductance microscopy (SICM) and is therefore expensive and not portable, but usually fixed to a specific microscope setup. The level of precision that these systems achieve is much higher than what is needed for the more robust nanoinjection process. We present Mobile Nanoinjection (MoNa), a portable, cost-efficient and easy to build system for the injection of single cells. Sacrificing unnecessary sub-nanometer accuracy and low ion current noise levels, we were able to inject single living cells with high accuracy. We determined the noise of the MoNa system and investigated the injection conditions for 16 prominent fluorescent labels and fluorophores. Further, we performed proof of concepts by injection of ATTO655-Phalloidin and MitoTracker Deep Red to living human osteosarcoma (U2OS) cells and of living adult human inferior turbinate stem cells (ITSC’s) following neuronal differentiation with the MoNa system. We achieved significant cost reductions of the nanoinjection technology and gained full portability and compatibility to most optical microscopes.

Original languageEnglish (US)
Article number5480
JournalScientific Reports
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2019
Externally publishedYes

Fingerprint

Costs and Cost Analysis
Injections
Noise
Ions
Phalloidine
Turbinates
Microinjections
Osteosarcoma
Microscopy
Stem Cells
Technology
Equipment and Supplies
Health

ASJC Scopus subject areas

  • General

Cite this

Simonis, M., Sandmeyer, A., Greiner, J., Kaltschmidt, B., Huser, T. R., & Hennig, S. (2019). MoNa – A Cost-Efficient, Portable System for the Nanoinjection of Living Cells. Scientific Reports, 9(1), [5480]. https://doi.org/10.1038/s41598-019-41648-6

MoNa – A Cost-Efficient, Portable System for the Nanoinjection of Living Cells. / Simonis, Matthias; Sandmeyer, Alice; Greiner, Johannes; Kaltschmidt, Barbara; Huser, Thomas R; Hennig, Simon.

In: Scientific Reports, Vol. 9, No. 1, 5480, 01.12.2019.

Research output: Contribution to journalArticle

Simonis, M, Sandmeyer, A, Greiner, J, Kaltschmidt, B, Huser, TR & Hennig, S 2019, 'MoNa – A Cost-Efficient, Portable System for the Nanoinjection of Living Cells', Scientific Reports, vol. 9, no. 1, 5480. https://doi.org/10.1038/s41598-019-41648-6
Simonis M, Sandmeyer A, Greiner J, Kaltschmidt B, Huser TR, Hennig S. MoNa – A Cost-Efficient, Portable System for the Nanoinjection of Living Cells. Scientific Reports. 2019 Dec 1;9(1). 5480. https://doi.org/10.1038/s41598-019-41648-6
Simonis, Matthias ; Sandmeyer, Alice ; Greiner, Johannes ; Kaltschmidt, Barbara ; Huser, Thomas R ; Hennig, Simon. / MoNa – A Cost-Efficient, Portable System for the Nanoinjection of Living Cells. In: Scientific Reports. 2019 ; Vol. 9, No. 1.
@article{89c634aa604c42289835c1ba20235b84,
title = "MoNa – A Cost-Efficient, Portable System for the Nanoinjection of Living Cells",
abstract = "Injection techniques to deliver macromolecules to cells such as microinjection have been around for decades with applications ranging from probing whole organisms to the injection of fluorescent molecules into single cells. A similar technique that has raised recent interest is nanoinjection. The pipettes used here are much smaller and allow for the precise deposition of molecules into single cells via electrokinetics with minimal influence on the cells’ health. Unfortunately, the equipment utilized for nanoinjection originates from scanning ion conductance microscopy (SICM) and is therefore expensive and not portable, but usually fixed to a specific microscope setup. The level of precision that these systems achieve is much higher than what is needed for the more robust nanoinjection process. We present Mobile Nanoinjection (MoNa), a portable, cost-efficient and easy to build system for the injection of single cells. Sacrificing unnecessary sub-nanometer accuracy and low ion current noise levels, we were able to inject single living cells with high accuracy. We determined the noise of the MoNa system and investigated the injection conditions for 16 prominent fluorescent labels and fluorophores. Further, we performed proof of concepts by injection of ATTO655-Phalloidin and MitoTracker Deep Red to living human osteosarcoma (U2OS) cells and of living adult human inferior turbinate stem cells (ITSC’s) following neuronal differentiation with the MoNa system. We achieved significant cost reductions of the nanoinjection technology and gained full portability and compatibility to most optical microscopes.",
author = "Matthias Simonis and Alice Sandmeyer and Johannes Greiner and Barbara Kaltschmidt and Huser, {Thomas R} and Simon Hennig",
year = "2019",
month = "12",
day = "1",
doi = "10.1038/s41598-019-41648-6",
language = "English (US)",
volume = "9",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - MoNa – A Cost-Efficient, Portable System for the Nanoinjection of Living Cells

AU - Simonis, Matthias

AU - Sandmeyer, Alice

AU - Greiner, Johannes

AU - Kaltschmidt, Barbara

AU - Huser, Thomas R

AU - Hennig, Simon

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Injection techniques to deliver macromolecules to cells such as microinjection have been around for decades with applications ranging from probing whole organisms to the injection of fluorescent molecules into single cells. A similar technique that has raised recent interest is nanoinjection. The pipettes used here are much smaller and allow for the precise deposition of molecules into single cells via electrokinetics with minimal influence on the cells’ health. Unfortunately, the equipment utilized for nanoinjection originates from scanning ion conductance microscopy (SICM) and is therefore expensive and not portable, but usually fixed to a specific microscope setup. The level of precision that these systems achieve is much higher than what is needed for the more robust nanoinjection process. We present Mobile Nanoinjection (MoNa), a portable, cost-efficient and easy to build system for the injection of single cells. Sacrificing unnecessary sub-nanometer accuracy and low ion current noise levels, we were able to inject single living cells with high accuracy. We determined the noise of the MoNa system and investigated the injection conditions for 16 prominent fluorescent labels and fluorophores. Further, we performed proof of concepts by injection of ATTO655-Phalloidin and MitoTracker Deep Red to living human osteosarcoma (U2OS) cells and of living adult human inferior turbinate stem cells (ITSC’s) following neuronal differentiation with the MoNa system. We achieved significant cost reductions of the nanoinjection technology and gained full portability and compatibility to most optical microscopes.

AB - Injection techniques to deliver macromolecules to cells such as microinjection have been around for decades with applications ranging from probing whole organisms to the injection of fluorescent molecules into single cells. A similar technique that has raised recent interest is nanoinjection. The pipettes used here are much smaller and allow for the precise deposition of molecules into single cells via electrokinetics with minimal influence on the cells’ health. Unfortunately, the equipment utilized for nanoinjection originates from scanning ion conductance microscopy (SICM) and is therefore expensive and not portable, but usually fixed to a specific microscope setup. The level of precision that these systems achieve is much higher than what is needed for the more robust nanoinjection process. We present Mobile Nanoinjection (MoNa), a portable, cost-efficient and easy to build system for the injection of single cells. Sacrificing unnecessary sub-nanometer accuracy and low ion current noise levels, we were able to inject single living cells with high accuracy. We determined the noise of the MoNa system and investigated the injection conditions for 16 prominent fluorescent labels and fluorophores. Further, we performed proof of concepts by injection of ATTO655-Phalloidin and MitoTracker Deep Red to living human osteosarcoma (U2OS) cells and of living adult human inferior turbinate stem cells (ITSC’s) following neuronal differentiation with the MoNa system. We achieved significant cost reductions of the nanoinjection technology and gained full portability and compatibility to most optical microscopes.

UR - http://www.scopus.com/inward/record.url?scp=85063750562&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85063750562&partnerID=8YFLogxK

U2 - 10.1038/s41598-019-41648-6

DO - 10.1038/s41598-019-41648-6

M3 - Article

C2 - 30940847

AN - SCOPUS:85063750562

VL - 9

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 5480

ER -