Molecular typing methods for the epidemiological identification of Clostridium difficile strains

Stuart H Cohen, Yajarayma J. Tang, Joseph Silva

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Toxigenic Clostridium difficile is the etiologic agent of C. difficile-associated diarrhea (CDAD), the most common cause of nosocomial diarrhea. Cross-infection between patients and transmission through the environment and medical personnel are important factors in the acquisition of CDAD. In order to understand differences in epidemiology and pathogenesis, a number of typing schemes have been developed. We will review the typing methods used to study the epidemiology of C. difficile infections and how they have evolved from a phenotypic identification to state of the art molecular methods, detecting genetic polymorphisms among strains. These molecular methods include PCR-based methods (arbitrarily primed-PCR [AP-PCR] and PCR ribotyping), restriction endonuclease analysis (REA) and pulse field gel electrophoresis (PFGE). The application, usefulness and feasibility of these methods are compared and discussed. Finally, the role of genomics as a tool to investigate CDAD is introduced.

Original languageEnglish (US)
Pages (from-to)61-70
Number of pages10
JournalExpert Review of Molecular Diagnostics
Volume1
Issue number1
StatePublished - 2001

Fingerprint

Epidemiologic Methods
Molecular Typing
Clostridium difficile
Diarrhea
Polymerase Chain Reaction
Epidemiology
Clostridium Infections
Ribotyping
DNA Restriction Enzymes
Genetic Polymorphisms
Cross Infection
Genomics
Electrophoresis
Gels

Keywords

  • AP-PCR
  • C. difficile
  • Epidemiology
  • Genotyping
  • PCR ribotyping
  • PFGE
  • Phenotypic methods
  • REA
  • Toxinotyping

ASJC Scopus subject areas

  • Genetics

Cite this

Molecular typing methods for the epidemiological identification of Clostridium difficile strains. / Cohen, Stuart H; Tang, Yajarayma J.; Silva, Joseph.

In: Expert Review of Molecular Diagnostics, Vol. 1, No. 1, 2001, p. 61-70.

Research output: Contribution to journalArticle

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