Molecular Structures and Interactions in the Yeast Kinetochore

U. S. Cho, K. D. Corbett, Jawdat Al-Bassam, J. J. Bellizzi, P. De Wulf, C. W. Espelin, J. J. Miranda, K. Simons, R. R. Wei, P. K. Sorger, S. C. Harrison

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Kinetochores are the elaborate protein assemblies that attach chromosomes to spindle microtubules in mitosis and meiosis. The kinetochores of point-centromere yeast appear to represent an elementary module, which repeats a number of times in kinetochores assembled on regional centromeres. Structural analyses of the discrete protein subcomplexes that make up the buddingyeast kinetochore have begun to reveal principles of kinetochore architecture and to uncover molecular mechanisms underlying functions such as transmission of tension and establishment and maintenance of bipolar attachment. The centromeric DNA is probably wrapped into a compact organization, not only by a conserved, centromeric nucleosome, but also by interactions among various other DNA-bound kinetochore components. The rod-like, heterotetrameric Ndc80 complex, roughly 600 Å long, appears to extend from the DNA-proximal assembly to the plus end of a microtubule, to which one end of the complex is known to bind. Ongoing structural studies will clarify the roles of a number of other well-defined complexes.

Original languageEnglish (US)
Pages (from-to)395-401
Number of pages7
JournalCold Spring Harbor Symposia on Quantitative Biology
Volume75
DOIs
StatePublished - 2010
Externally publishedYes

Fingerprint

Kinetochores
Molecular interactions
Molecular Structure
Yeast
Molecular structure
Yeasts
DNA
Nucleosomes
Centromere
Chromosomes
Microtubules
Proteins
Meiosis
Mitosis
Maintenance

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Biochemistry

Cite this

Cho, U. S., Corbett, K. D., Al-Bassam, J., Bellizzi, J. J., De Wulf, P., Espelin, C. W., ... Harrison, S. C. (2010). Molecular Structures and Interactions in the Yeast Kinetochore. Cold Spring Harbor Symposia on Quantitative Biology, 75, 395-401. https://doi.org/10.1101/sqb.2010.75.040

Molecular Structures and Interactions in the Yeast Kinetochore. / Cho, U. S.; Corbett, K. D.; Al-Bassam, Jawdat; Bellizzi, J. J.; De Wulf, P.; Espelin, C. W.; Miranda, J. J.; Simons, K.; Wei, R. R.; Sorger, P. K.; Harrison, S. C.

In: Cold Spring Harbor Symposia on Quantitative Biology, Vol. 75, 2010, p. 395-401.

Research output: Contribution to journalArticle

Cho, US, Corbett, KD, Al-Bassam, J, Bellizzi, JJ, De Wulf, P, Espelin, CW, Miranda, JJ, Simons, K, Wei, RR, Sorger, PK & Harrison, SC 2010, 'Molecular Structures and Interactions in the Yeast Kinetochore', Cold Spring Harbor Symposia on Quantitative Biology, vol. 75, pp. 395-401. https://doi.org/10.1101/sqb.2010.75.040
Cho, U. S. ; Corbett, K. D. ; Al-Bassam, Jawdat ; Bellizzi, J. J. ; De Wulf, P. ; Espelin, C. W. ; Miranda, J. J. ; Simons, K. ; Wei, R. R. ; Sorger, P. K. ; Harrison, S. C. / Molecular Structures and Interactions in the Yeast Kinetochore. In: Cold Spring Harbor Symposia on Quantitative Biology. 2010 ; Vol. 75. pp. 395-401.
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