Molecular NMR T2 values can predict cartilage stress-relaxation parameters

Ronald K. June, David P Fyhrie

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Articular cartilage lines synovial joints and functions as a low-friction deformable tissue to enable smooth and stable joint articulation. The objective of this study was to determine the relationships between cartilage stress-relaxation properties and the collagen and GAG NMR transverse relaxation times (T2) toward understanding mechanisms of cartilage viscoelasticity. Stress-relaxation tests were performed on both cultured and enzymatically digested bovine cartilage, followed by measurements of both the collagen and GAG T2 using the Call-Purcell-Meiboom-Gill pulse sequence. The peak and equilibrium stresses were correlated with the GAG T2, and the stress-relaxation time constant was correlated with the collagen T2. Multiple linear regression models were successful in using the specific T2 values to predict the stress-relaxation properties. As a model of osteoarthritis, enzymatic digestion with collagenase and testicular hyaluronidase had weak effects on T2 values. These data present a complex picture of cartilage mechanical behavior, with cartilage stiffness associated with the GAG T2 values and the stress-relaxation time constant associated with the collagen T2.

Original languageEnglish (US)
Pages (from-to)57-61
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume377
Issue number1
DOIs
StatePublished - Dec 5 2008

Keywords

  • Cartilage
  • Cartilage material properties
  • Cartilage viscoelasticity
  • Flow-independent viscoelasticity
  • Matrix viscoelasticty
  • Polymer dynamics
  • Transverse relaxation time

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

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