Molecular mechanisms of sister-chromatid exchange

David M. Wilson, Larry H. Thompson

Research output: Contribution to journalArticle

189 Scopus citations

Abstract

Sister-chromatid exchange (SCE) is the process whereby, during DNA replication, two sister chromatids break and rejoin with one another, physically exchanging regions of the parental strands in the duplicated chromosomes. This process is considered to be conservative and error-free, since no information is generally altered during reciprocal interchange by homologous recombination. Upon the advent of non-radiolabel detection methods for SCE, such events were used as genetic indicators for potential genotoxins/mutagens in laboratory toxicology tests, since, as we now know, most forms of DNA damage induce chromatid exchange upon replication fork collapse. Much of our present understanding of the mechanisms of SCE stems from studies involving nonhuman vertebrate cell lines that are defective in processes of DNA repair and/or recombination. In this article, we present a historical perspective of studies spearheaded by Dr. Anthony V. Carrano and colleagues focusing on SCE as a genetic outcome, and the role of the single-strand break DNA repair protein XRCC1 in suppressing SCE. A more general overview of the cellular processes and key protein "effectors" that regulate the manifestation of SCE is also presented.

Original languageEnglish (US)
Pages (from-to)11-23
Number of pages13
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume616
Issue number1-2
DOIs
StatePublished - Mar 1 2007
Externally publishedYes

Keywords

  • Bloom syndrome
  • CHO EM9
  • DNA replication forks
  • Homologous recombination
  • Single-strand break DNA repair
  • Sister-chromatid exchange
  • XRCC1

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis
  • Molecular Biology

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