Molecular markers reliably predict post-harvest deterioration of fresh-cut lettuce in modified atmosphere packaging

Ivan Simko, Ryan J. Hayes, Maria Jose Truco, Richard W Michelmore, Rudie Antonise, Mark Massoudi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Fresh-cut lettuce is popular, but highly perishable product. Genetic studies of two bi-parental populations derived from crossing parents with rapid and slow rates of deterioration showed that the deterioration rate is a heritable trait (broad spectrum heritability, H 2 of 0.56-0.87). The major genetic determinant of the deterioration rate in both populations was the quantitative trait locus (QTL), qSL4, located on linkage group 4. This QTL explained 40-74% of the total phenotypic variation of the trait in the two populations. Saturating the qSL4 region with single-nucleotide (SNP) markers allowed detection of six haplotypes in a set of 16 lettuce accessions with different rates of deterioration. Three of the haplotypes were always associated with very rapid rates of deterioration, while the other three haplotypes were associated with slow rates of deterioration. Two SNPs located 53 bp apart were sufficient to separate the 16 accessions into two groups with different rates of deterioration. The accuracy of markers-trait association was subsequently tested on 350 plants from seven F2 families that originated from crossing parents with different rates of deterioration. The H 2 of deterioration rate in these seven families ranged from 0.64 to 0.90. The SNP-based analysis accurately identified individuals with rapid, intermediate, and slow rates of deterioration in each family. Intermediate rate of deterioration was found in individuals having heterozygous alleles at qSL4, indicating an additive effect of the alleles. The assay can be used for fast, accurate, and reliable identification of deterioration rate after processing for salad.

Original languageEnglish (US)
Article number21
JournalHorticulture Research
Volume5
Issue number1
DOIs
StatePublished - Dec 1 2018

Fingerprint

Modified atmosphere packaging
Lettuce
modified atmosphere packaging
Product Packaging
Atmosphere
lettuce
Haplotypes
Single Nucleotide Polymorphism
Deterioration
Quantitative Trait Loci
deterioration
genetic markers
Alleles
Population
Nucleotides
haplotypes
quantitative trait loci
perishable products
alleles
salads

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Genetics
  • Plant Science
  • Horticulture

Cite this

Molecular markers reliably predict post-harvest deterioration of fresh-cut lettuce in modified atmosphere packaging. / Simko, Ivan; Hayes, Ryan J.; Truco, Maria Jose; Michelmore, Richard W; Antonise, Rudie; Massoudi, Mark.

In: Horticulture Research, Vol. 5, No. 1, 21, 01.12.2018.

Research output: Contribution to journalArticle

Simko, Ivan ; Hayes, Ryan J. ; Truco, Maria Jose ; Michelmore, Richard W ; Antonise, Rudie ; Massoudi, Mark. / Molecular markers reliably predict post-harvest deterioration of fresh-cut lettuce in modified atmosphere packaging. In: Horticulture Research. 2018 ; Vol. 5, No. 1.
@article{baf63043e0e64c1bb7836e688a5e0099,
title = "Molecular markers reliably predict post-harvest deterioration of fresh-cut lettuce in modified atmosphere packaging",
abstract = "Fresh-cut lettuce is popular, but highly perishable product. Genetic studies of two bi-parental populations derived from crossing parents with rapid and slow rates of deterioration showed that the deterioration rate is a heritable trait (broad spectrum heritability, H 2 of 0.56-0.87). The major genetic determinant of the deterioration rate in both populations was the quantitative trait locus (QTL), qSL4, located on linkage group 4. This QTL explained 40-74{\%} of the total phenotypic variation of the trait in the two populations. Saturating the qSL4 region with single-nucleotide (SNP) markers allowed detection of six haplotypes in a set of 16 lettuce accessions with different rates of deterioration. Three of the haplotypes were always associated with very rapid rates of deterioration, while the other three haplotypes were associated with slow rates of deterioration. Two SNPs located 53 bp apart were sufficient to separate the 16 accessions into two groups with different rates of deterioration. The accuracy of markers-trait association was subsequently tested on 350 plants from seven F2 families that originated from crossing parents with different rates of deterioration. The H 2 of deterioration rate in these seven families ranged from 0.64 to 0.90. The SNP-based analysis accurately identified individuals with rapid, intermediate, and slow rates of deterioration in each family. Intermediate rate of deterioration was found in individuals having heterozygous alleles at qSL4, indicating an additive effect of the alleles. The assay can be used for fast, accurate, and reliable identification of deterioration rate after processing for salad.",
author = "Ivan Simko and Hayes, {Ryan J.} and Truco, {Maria Jose} and Michelmore, {Richard W} and Rudie Antonise and Mark Massoudi",
year = "2018",
month = "12",
day = "1",
doi = "10.1038/s41438-018-0022-5",
language = "English (US)",
volume = "5",
journal = "Horticulture Research",
issn = "2052-7276",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Molecular markers reliably predict post-harvest deterioration of fresh-cut lettuce in modified atmosphere packaging

AU - Simko, Ivan

AU - Hayes, Ryan J.

AU - Truco, Maria Jose

AU - Michelmore, Richard W

AU - Antonise, Rudie

AU - Massoudi, Mark

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Fresh-cut lettuce is popular, but highly perishable product. Genetic studies of two bi-parental populations derived from crossing parents with rapid and slow rates of deterioration showed that the deterioration rate is a heritable trait (broad spectrum heritability, H 2 of 0.56-0.87). The major genetic determinant of the deterioration rate in both populations was the quantitative trait locus (QTL), qSL4, located on linkage group 4. This QTL explained 40-74% of the total phenotypic variation of the trait in the two populations. Saturating the qSL4 region with single-nucleotide (SNP) markers allowed detection of six haplotypes in a set of 16 lettuce accessions with different rates of deterioration. Three of the haplotypes were always associated with very rapid rates of deterioration, while the other three haplotypes were associated with slow rates of deterioration. Two SNPs located 53 bp apart were sufficient to separate the 16 accessions into two groups with different rates of deterioration. The accuracy of markers-trait association was subsequently tested on 350 plants from seven F2 families that originated from crossing parents with different rates of deterioration. The H 2 of deterioration rate in these seven families ranged from 0.64 to 0.90. The SNP-based analysis accurately identified individuals with rapid, intermediate, and slow rates of deterioration in each family. Intermediate rate of deterioration was found in individuals having heterozygous alleles at qSL4, indicating an additive effect of the alleles. The assay can be used for fast, accurate, and reliable identification of deterioration rate after processing for salad.

AB - Fresh-cut lettuce is popular, but highly perishable product. Genetic studies of two bi-parental populations derived from crossing parents with rapid and slow rates of deterioration showed that the deterioration rate is a heritable trait (broad spectrum heritability, H 2 of 0.56-0.87). The major genetic determinant of the deterioration rate in both populations was the quantitative trait locus (QTL), qSL4, located on linkage group 4. This QTL explained 40-74% of the total phenotypic variation of the trait in the two populations. Saturating the qSL4 region with single-nucleotide (SNP) markers allowed detection of six haplotypes in a set of 16 lettuce accessions with different rates of deterioration. Three of the haplotypes were always associated with very rapid rates of deterioration, while the other three haplotypes were associated with slow rates of deterioration. Two SNPs located 53 bp apart were sufficient to separate the 16 accessions into two groups with different rates of deterioration. The accuracy of markers-trait association was subsequently tested on 350 plants from seven F2 families that originated from crossing parents with different rates of deterioration. The H 2 of deterioration rate in these seven families ranged from 0.64 to 0.90. The SNP-based analysis accurately identified individuals with rapid, intermediate, and slow rates of deterioration in each family. Intermediate rate of deterioration was found in individuals having heterozygous alleles at qSL4, indicating an additive effect of the alleles. The assay can be used for fast, accurate, and reliable identification of deterioration rate after processing for salad.

UR - http://www.scopus.com/inward/record.url?scp=85044769879&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85044769879&partnerID=8YFLogxK

U2 - 10.1038/s41438-018-0022-5

DO - 10.1038/s41438-018-0022-5

M3 - Article

AN - SCOPUS:85044769879

VL - 5

JO - Horticulture Research

JF - Horticulture Research

SN - 2052-7276

IS - 1

M1 - 21

ER -