Abstract
Whole genome expression microarrays can be used to study gene expression in blood, which comes in part from leukocytes, immature platelets, and red blood cells. Since these cells are important in the pathogenesis of stroke, RNA provides an index of these cellular responses to stroke. Our studies in rats have shown specific gene expression changes 24 hours after ischemic stroke, hemorrhage, status epilepticus, hypoxia, hypoglycemia, global ischemia, and following brief focal ischemia that simulated transient ischemic attacks in humans. Human studies show gene expression changes following ischemic stroke. These gene profiles predict a second cohort with 90% sensitivity and specificity. Gene profiles for ischemic stroke caused by large-vessel atherosclerosis and cardioembolism have been described that predict a second cohort with 85% sensitivity and specificity. Atherosclerotic genes were associated with clotting, platelets, and monocytes, and cardioembolic genes were associated with inflammation, infection, and neutrophils. These gene profiles predicted the cause of stroke in 58% of cryptogenic patients. These studies will provide diagnostic, prognostic, and therapeutic markers, and will advance our understanding of stroke in humans. New techniques to measure all coding and noncoding RNAs along with alternatively spliced transcripts will markedly advance molecular studies of human stroke.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1513-1531 |
| Number of pages | 19 |
| Journal | Journal of Cerebral Blood Flow and Metabolism |
| Volume | 31 |
| Issue number | 7 |
| DOIs | |
| State | Published - 2011 |
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Keywords
- brain
- gene expression profiling
- ischemia
- microarrays
ASJC Scopus subject areas
- Neurology
- Clinical Neurology
- Cardiology and Cardiovascular Medicine
Cite this
Molecular markers and mechanisms of stroke : RNA studies of blood in animals and humans. / Sharp, Frank R; Jickling, Glen C.; Stamova, Boryana; Tian, Yingfang; Zhan, Xinhua; Liu, Da; Kuczynski, Beth; Cox, Christopher D.; Ander, Bradley.
In: Journal of Cerebral Blood Flow and Metabolism, Vol. 31, No. 7, 2011, p. 1513-1531.Research output: Contribution to journal › Review article
}
TY - JOUR
T1 - Molecular markers and mechanisms of stroke
T2 - RNA studies of blood in animals and humans
AU - Sharp, Frank R
AU - Jickling, Glen C.
AU - Stamova, Boryana
AU - Tian, Yingfang
AU - Zhan, Xinhua
AU - Liu, Da
AU - Kuczynski, Beth
AU - Cox, Christopher D.
AU - Ander, Bradley
PY - 2011
Y1 - 2011
N2 - Whole genome expression microarrays can be used to study gene expression in blood, which comes in part from leukocytes, immature platelets, and red blood cells. Since these cells are important in the pathogenesis of stroke, RNA provides an index of these cellular responses to stroke. Our studies in rats have shown specific gene expression changes 24 hours after ischemic stroke, hemorrhage, status epilepticus, hypoxia, hypoglycemia, global ischemia, and following brief focal ischemia that simulated transient ischemic attacks in humans. Human studies show gene expression changes following ischemic stroke. These gene profiles predict a second cohort with 90% sensitivity and specificity. Gene profiles for ischemic stroke caused by large-vessel atherosclerosis and cardioembolism have been described that predict a second cohort with 85% sensitivity and specificity. Atherosclerotic genes were associated with clotting, platelets, and monocytes, and cardioembolic genes were associated with inflammation, infection, and neutrophils. These gene profiles predicted the cause of stroke in 58% of cryptogenic patients. These studies will provide diagnostic, prognostic, and therapeutic markers, and will advance our understanding of stroke in humans. New techniques to measure all coding and noncoding RNAs along with alternatively spliced transcripts will markedly advance molecular studies of human stroke.
AB - Whole genome expression microarrays can be used to study gene expression in blood, which comes in part from leukocytes, immature platelets, and red blood cells. Since these cells are important in the pathogenesis of stroke, RNA provides an index of these cellular responses to stroke. Our studies in rats have shown specific gene expression changes 24 hours after ischemic stroke, hemorrhage, status epilepticus, hypoxia, hypoglycemia, global ischemia, and following brief focal ischemia that simulated transient ischemic attacks in humans. Human studies show gene expression changes following ischemic stroke. These gene profiles predict a second cohort with 90% sensitivity and specificity. Gene profiles for ischemic stroke caused by large-vessel atherosclerosis and cardioembolism have been described that predict a second cohort with 85% sensitivity and specificity. Atherosclerotic genes were associated with clotting, platelets, and monocytes, and cardioembolic genes were associated with inflammation, infection, and neutrophils. These gene profiles predicted the cause of stroke in 58% of cryptogenic patients. These studies will provide diagnostic, prognostic, and therapeutic markers, and will advance our understanding of stroke in humans. New techniques to measure all coding and noncoding RNAs along with alternatively spliced transcripts will markedly advance molecular studies of human stroke.
KW - brain
KW - gene expression profiling
KW - ischemia
KW - microarrays
UR - http://www.scopus.com/inward/record.url?scp=85027927887&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85027927887&partnerID=8YFLogxK
U2 - 10.1038/jcbfm.2011.45
DO - 10.1038/jcbfm.2011.45
M3 - Review article
AN - SCOPUS:85027927887
VL - 31
SP - 1513
EP - 1531
JO - Journal of Cerebral Blood Flow and Metabolism
JF - Journal of Cerebral Blood Flow and Metabolism
SN - 0271-678X
IS - 7
ER -