Abstract
Persistent Escherichia coli urinary tract infection (UTI) in dogs is a frustrating clinical problem. Affected dogs often appear to fail to respond to therapy or to reacquire infection shortly after therapy is completed. Urovirulence factors (UVFs) of the infecting E coli, antibiotic resistance, and tissue colonization may be contributory but have not been evaluated in dogs with persistent E coli UTI. In this study, the strain types of E coli in dogs with persistent UTI were evaluated with pulsed-field gel electrophoresis (PFGE) to determine whether persistence was due to acquisition of new isolates or failure to eradicate existing isolates. UVFs in these isolates, assessed by polymerase chain reaction, and antibiograms were correlated with treatment outcome in these dogs. Results documented a mixed pattern: 9 dogs remained chronically infected with 1 or 2 strains, each with distinct reproducible UVFs, but 1 dog was infected with numerous unrelated E coli strains over time. Two dogs had a mixed pattern, consisting of 1 or more episodes of persistent E coli infection attributable to a single strain in addition to episodes caused by unrelated strains. Many isolates had no detectable UVFs, highlighting the likely importance of impaired colonization resistance in the affected dogs. Antibiotic resistance was common, often in response to previous treatments, especially with trimethoprim-sulfamethoxazole. Antibiotic resistance patterns differed significantly within PFGE strain types, suggesting lateral acquisition of resistance plasmids or integrons. These results can be used to help guide testing for and management of persistent E coli UTI in dogs.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 301-306 |
| Number of pages | 6 |
| Journal | Journal of Veterinary Internal Medicine |
| Volume | 18 |
| Issue number | 3 |
| DOIs | |
| State | Published - May 2004 |
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Keywords
- Antibiotic susceptibility
- Canine
- Polymerase chain reaction
- Pulsed-field gel electrophoresis
ASJC Scopus subject areas
- veterinary(all)
Cite this
Molecular investigation of Escherichia coli strains associated with apparently persistent urinary tract infection in dogs. / Drazenovich, Nicole; Ling, Gerald V.; Foley, Janet E.
In: Journal of Veterinary Internal Medicine, Vol. 18, No. 3, 05.2004, p. 301-306.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular investigation of Escherichia coli strains associated with apparently persistent urinary tract infection in dogs
AU - Drazenovich, Nicole
AU - Ling, Gerald V.
AU - Foley, Janet E
PY - 2004/5
Y1 - 2004/5
N2 - Persistent Escherichia coli urinary tract infection (UTI) in dogs is a frustrating clinical problem. Affected dogs often appear to fail to respond to therapy or to reacquire infection shortly after therapy is completed. Urovirulence factors (UVFs) of the infecting E coli, antibiotic resistance, and tissue colonization may be contributory but have not been evaluated in dogs with persistent E coli UTI. In this study, the strain types of E coli in dogs with persistent UTI were evaluated with pulsed-field gel electrophoresis (PFGE) to determine whether persistence was due to acquisition of new isolates or failure to eradicate existing isolates. UVFs in these isolates, assessed by polymerase chain reaction, and antibiograms were correlated with treatment outcome in these dogs. Results documented a mixed pattern: 9 dogs remained chronically infected with 1 or 2 strains, each with distinct reproducible UVFs, but 1 dog was infected with numerous unrelated E coli strains over time. Two dogs had a mixed pattern, consisting of 1 or more episodes of persistent E coli infection attributable to a single strain in addition to episodes caused by unrelated strains. Many isolates had no detectable UVFs, highlighting the likely importance of impaired colonization resistance in the affected dogs. Antibiotic resistance was common, often in response to previous treatments, especially with trimethoprim-sulfamethoxazole. Antibiotic resistance patterns differed significantly within PFGE strain types, suggesting lateral acquisition of resistance plasmids or integrons. These results can be used to help guide testing for and management of persistent E coli UTI in dogs.
AB - Persistent Escherichia coli urinary tract infection (UTI) in dogs is a frustrating clinical problem. Affected dogs often appear to fail to respond to therapy or to reacquire infection shortly after therapy is completed. Urovirulence factors (UVFs) of the infecting E coli, antibiotic resistance, and tissue colonization may be contributory but have not been evaluated in dogs with persistent E coli UTI. In this study, the strain types of E coli in dogs with persistent UTI were evaluated with pulsed-field gel electrophoresis (PFGE) to determine whether persistence was due to acquisition of new isolates or failure to eradicate existing isolates. UVFs in these isolates, assessed by polymerase chain reaction, and antibiograms were correlated with treatment outcome in these dogs. Results documented a mixed pattern: 9 dogs remained chronically infected with 1 or 2 strains, each with distinct reproducible UVFs, but 1 dog was infected with numerous unrelated E coli strains over time. Two dogs had a mixed pattern, consisting of 1 or more episodes of persistent E coli infection attributable to a single strain in addition to episodes caused by unrelated strains. Many isolates had no detectable UVFs, highlighting the likely importance of impaired colonization resistance in the affected dogs. Antibiotic resistance was common, often in response to previous treatments, especially with trimethoprim-sulfamethoxazole. Antibiotic resistance patterns differed significantly within PFGE strain types, suggesting lateral acquisition of resistance plasmids or integrons. These results can be used to help guide testing for and management of persistent E coli UTI in dogs.
KW - Antibiotic susceptibility
KW - Canine
KW - Polymerase chain reaction
KW - Pulsed-field gel electrophoresis
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UR - http://www.scopus.com/inward/citedby.url?scp=2942594811&partnerID=8YFLogxK
U2 - 10.1892/0891-6640(2004)18<301:MIOECS>2.0.CO;2
DO - 10.1892/0891-6640(2004)18<301:MIOECS>2.0.CO;2
M3 - Article
C2 - 15188815
AN - SCOPUS:2942594811
VL - 18
SP - 301
EP - 306
JO - Journal of Veterinary Internal Medicine
JF - Journal of Veterinary Internal Medicine
SN - 0891-6640
IS - 3
ER -