Molecular identity and functional properties of a novel T-type Ca 2+ channel cloned from the sensory epithelia of the mouse inner ear

Liping Nie, Jun Zhu, Michael Anne Gratton, Amy Liao, Karen J. Mu, Wolfgang Nonner, Guy P. Richardson, Ebenezer N. Yamoah

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The molecular identity of non-Cav1.3 channels in auditory and vestibular hair cells has remained obscure, yet the evidence in support of their roles to promote diverse Ca2+-dependent functions is indisputable. Recently, a transient Cav3.1 current that serves as a functional signature for the development and regeneration of hair cells has been identified in the chicken basilar papilla. The Cav3.1 current promotes spontaneous activity of the developing hair cell, which may be essential for synapse formation. Here, we have isolated and sequenced the fulllength complementary DNA of a distinct isoform of Cav3.1 in the mouse inner ear. The channel is derived from alternative splicing of exon14, exon25A, exon34, and exon35. Functional expression of the channel in Xenopus oocytes yielded Ca2+ currents, which have a permeation phenotype consistent with T-type channels. However, unlike most multiion channels, the T-type channel does not exhibit the anomalous mole fraction effect, possibly reflecting comparable permeation properties of divalent cations. The Cav3.1 channel was expressed in sensory and nonsensory epithelia of the inner ear. Moreover, there are profound changes in the expression levels during development. The differential expression of the channel during development and the pharmacology of the inner ear Cav3.1 channel may have contributed to the difficulties associated with identification of the non-Cav1.3 currents.

Original languageEnglish (US)
Pages (from-to)2287-2299
Number of pages13
JournalJournal of Neurophysiology
Volume100
Issue number4
DOIs
StatePublished - Oct 2008

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Inner Ear
Epithelium
Auditory Hair Cells
Vestibular Hair Cells
Organ of Corti
Divalent Cations
Alternative Splicing
Xenopus
Synapses
Oocytes
Regeneration
Chickens
Protein Isoforms
Complementary DNA
Pharmacology
Phenotype

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)

Cite this

Molecular identity and functional properties of a novel T-type Ca 2+ channel cloned from the sensory epithelia of the mouse inner ear. / Nie, Liping; Zhu, Jun; Gratton, Michael Anne; Liao, Amy; Mu, Karen J.; Nonner, Wolfgang; Richardson, Guy P.; Yamoah, Ebenezer N.

In: Journal of Neurophysiology, Vol. 100, No. 4, 10.2008, p. 2287-2299.

Research output: Contribution to journalArticle

Nie, Liping ; Zhu, Jun ; Gratton, Michael Anne ; Liao, Amy ; Mu, Karen J. ; Nonner, Wolfgang ; Richardson, Guy P. ; Yamoah, Ebenezer N. / Molecular identity and functional properties of a novel T-type Ca 2+ channel cloned from the sensory epithelia of the mouse inner ear. In: Journal of Neurophysiology. 2008 ; Vol. 100, No. 4. pp. 2287-2299.
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