Molecular identification of clinical and environmental avian Aspergillus isolates

Raquel Sabino, Julia Burco, Joana Valente, Cristina Veríssimo, Karl V. Clemons, David A. Stevens, Lisa A Tell

Research output: Contribution to journalArticle

Abstract

Aspergillosis causes high morbidity and mortality in avian species. The main goal of this study was to use molecular techniques to identify Aspergillus species collected from different avian species with aspergillosis. A subsample of those isolates was also screened for resistance to itraconazole. Over a 2-year period, clinical samples were recovered from 44 birds with clinical signs of the disease, clinical pathology results suspicious of aspergillosis, or from birds that died from Aspergillus spp. infection. Environmental sampling was also performed in seabird rehabilitation centers and natural seabird environments. Seventy-seven isolates (43 clinical and 34 environmental) were identified as Aspergillus fumigatus sensu stricto. No cryptic species from the Fumigati section were detected. Two environmental isolates were identified as Aspergillus nidulans var. dentatus and Aspergillus spinulosporus. None of the Aspergillus isolates tested were resistant to itraconazole. Our study emphasizes the dominant association of Aspergillus fumigatus sensu stricto in avian mycoses and shows the lack of itraconazole resistance in the studied isolates.

Original languageEnglish (US)
JournalArchives of Microbiology
DOIs
StateAccepted/In press - Jan 1 2019

Fingerprint

Aspergillus
Itraconazole
Aspergillosis
Aspergillus fumigatus
Birds
Aspergillus nidulans
Rehabilitation Centers
Clinical Pathology
Mycoses
Morbidity
Pathology
Mortality
Patient rehabilitation
Infection
Sampling

Keywords

  • Aspergillosis
  • Aspergillus
  • Birds
  • Cryptic species
  • Drug susceptibility

ASJC Scopus subject areas

  • Microbiology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Sabino, R., Burco, J., Valente, J., Veríssimo, C., Clemons, K. V., Stevens, D. A., & Tell, L. A. (Accepted/In press). Molecular identification of clinical and environmental avian Aspergillus isolates. Archives of Microbiology. https://doi.org/10.1007/s00203-019-01618-y

Molecular identification of clinical and environmental avian Aspergillus isolates. / Sabino, Raquel; Burco, Julia; Valente, Joana; Veríssimo, Cristina; Clemons, Karl V.; Stevens, David A.; Tell, Lisa A.

In: Archives of Microbiology, 01.01.2019.

Research output: Contribution to journalArticle

Sabino, Raquel ; Burco, Julia ; Valente, Joana ; Veríssimo, Cristina ; Clemons, Karl V. ; Stevens, David A. ; Tell, Lisa A. / Molecular identification of clinical and environmental avian Aspergillus isolates. In: Archives of Microbiology. 2019.
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