Molecular evidence of Bartonella spp. in Questing adult Ixodes pacificus ticks in California

C. C. Chang, Bruno B Chomel, R. W. Kasten, V. Romano, N. Tietze

Research output: Contribution to journalArticle

159 Citations (Scopus)

Abstract

Ticks are the vectors of many zoonotic diseases in the United States, including Lyme disease, human monocytic and granulocytic ehrlichioses, and Rocky Mountain spotted fever. Most known Bartonella species are arthropod borne. Therefore, it is important to determine if some Bartonella species, which are emerging pathogens, could be carried or transmitted by ticks. In this study, adult Ixodes pacificus ticks were collected by flagging vegetation in three sites in Santa Clara County, Calif. PCR-restriction fragment length polymorphism and partial sequencing of 273 bp of the gltA gene were applied for Bartonella identification. Twenty-nine (19.2%) of 151 individually tested ticks were PCR positive for Bartonella. Male ticks were more likely to be infected with Bartonella than female ticks (26 versus 12%, P = 0.05). None of the nine ticks collected at Baird Ranch was PCR positive for Bartonella. However, 7 (50%) of 14 ticks from Red Fern Ranch and 22 (17%) of 128 ticks from the Windy Hill Open Space Reserve were infected with Bartonella. In these infected ticks, molecular analysis showed a variety of Bartonella strains, which were closely related to a cattle Bartonella strain and to several known human-pathogenic Bartonella species and subspecies: Bartonella henselae, B. quintana, B. washoensis, and B. vinsonii subsp. berkhoffii. These findings indicate that I. pacificus ticks may play an important role in Bartonella transmission among animals and humans.

Original languageEnglish (US)
Pages (from-to)1221-1226
Number of pages6
JournalJournal of Clinical Microbiology
Volume39
Issue number4
DOIs
StatePublished - 2001

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Bartonella
Ixodes
Ticks
Polymerase Chain Reaction
Rocky Mountain Spotted Fever
Bartonella henselae
Ehrlichiosis
Ferns
Lyme Disease
Arthropods
Zoonoses
Restriction Fragment Length Polymorphisms

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Molecular evidence of Bartonella spp. in Questing adult Ixodes pacificus ticks in California. / Chang, C. C.; Chomel, Bruno B; Kasten, R. W.; Romano, V.; Tietze, N.

In: Journal of Clinical Microbiology, Vol. 39, No. 4, 2001, p. 1221-1226.

Research output: Contribution to journalArticle

Chang, C. C. ; Chomel, Bruno B ; Kasten, R. W. ; Romano, V. ; Tietze, N. / Molecular evidence of Bartonella spp. in Questing adult Ixodes pacificus ticks in California. In: Journal of Clinical Microbiology. 2001 ; Vol. 39, No. 4. pp. 1221-1226.
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