Molecular epidemiology of HIV type 1 subtypes in Rwanda

Kimdar S. Kemal, Kathryn Anastos, Barbara Weiser, Christina M. Ramirez, Qiuhu Shi, Harold Burger

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

HIV-1 infection is characterized by genetic diversity, with multiple subtypes and recombinant variants circulating, particularly in sub-Saharan Africa. During the Rwandan genocide, many women experienced multiple rapes and some became HIV-1 infected. We studied plasma and peripheral blood mononuclear cells (PBMCs) from 30 infected women comprising two exposure groups: those with numerous contacts, raped multiple times, and women with one lifetime sexual partner and no history of rape. Population-based sequences from gag, pol, and env genes were analyzed to determine HIV-1 subtypes and intersubtype recombination. Individual plasma-derived variants from 12 women were also analyzed. Subtype A was found in 24/30 (80%), intersubtype recombination (AC and AD) in 4/30 (13%), and subtypes C and D in 1/30 each. In two subjects, the pattern of HIV-1 recombination differed between plasma and PBMC-derived sequences. Intersubtype recombination was common, although there were no significant differences in subtype or recombination rates between exposure groups.

Original languageEnglish (US)
Pages (from-to)957-962
Number of pages6
JournalAIDS Research and Human Retroviruses
Volume29
Issue number6
DOIs
StatePublished - Jun 1 2013

    Fingerprint

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Infectious Diseases

Cite this