Molecular epidemiology of Corynebacterium pseudotuberculosis isolated from horses in California

Dionei J. Haas, Elaine M.S. Dorneles, Sharon Spier, Scott P. Carroll, Judy Edman, Vasco A. Azevedo, Marcos B. Heinemann, Andrey P. Lage

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Corynebacterium pseudotuberculosis biovar Equi is an important pathogen of horses. It is increasing in frequency in the United States, and is responsible for various clinical forms of infection, including external abscesses, internal abscesses of the abdominal or thoracic cavities, and ulcerative lymphangitis. The host/pathogen factors dictating the form or severity of infection are currently unknown. Our recent investigations have shown that genotyping C. pseudotuberculosis isolates using enterobacterial repetitive intergenic consensus (ERIC)-PCR is useful for understanding the evolutionary genetics of the species as well for molecular epidemiology studies. The aims of the present study were to assess (i) the genetic diversity of C. pseudotuberculosis strains isolated from horses in California, United States and (ii) the epidemiologic relationships among isolates. One hundred and seven C. pseudotuberculosis biovar Equi isolates from ninety-five horses, and two C. pseudotuberculosis biovar Ovis strains, C. pseudotuberculosis ATCC 19410T type strain and C. pseudotuberculosis 1002 vaccine strain, were fingerprinted using the ERIC 1 + 2-PCR. C. pseudotuberculosis isolated from horses showed a high genetic diversity, clustering in twenty-seven genotypes with a diversity index of 0.91. Minimal spanning tree showed four major clonal complexes with a pattern of temporal clustering. Strains isolated from the same horse showed identical ERIC 1 + 2-PCR genotype, with the exception of two strains isolated from the same animal that showed distinct genotypes, suggesting a co-infection. We found no strong genetic signals related to clinical form (including internal versus external infections). However, temporal clustering of genotypes was observed.

Original languageEnglish (US)
Pages (from-to)186-194
Number of pages9
JournalInfection, Genetics and Evolution
Volume49
DOIs
StatePublished - Apr 1 2017

Fingerprint

Corynebacterium pseudotuberculosis
molecular epidemiology
Molecular Epidemiology
epidemiology
horse
Horses
horses
genotype
Genotype
Cluster Analysis
pathogen
Equus
abscess
Polymerase Chain Reaction
Abscess
vaccine
diversity index
lymphangitis
Infection
Lymphangitis

Keywords

  • Co-infection
  • ERIC 1 + 2-PCR
  • Genotyping
  • Temporal clustering pattern
  • Ulcerative lymphangitis

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Molecular epidemiology of Corynebacterium pseudotuberculosis isolated from horses in California. / Haas, Dionei J.; Dorneles, Elaine M.S.; Spier, Sharon; Carroll, Scott P.; Edman, Judy; Azevedo, Vasco A.; Heinemann, Marcos B.; Lage, Andrey P.

In: Infection, Genetics and Evolution, Vol. 49, 01.04.2017, p. 186-194.

Research output: Contribution to journalArticle

Haas, Dionei J. ; Dorneles, Elaine M.S. ; Spier, Sharon ; Carroll, Scott P. ; Edman, Judy ; Azevedo, Vasco A. ; Heinemann, Marcos B. ; Lage, Andrey P. / Molecular epidemiology of Corynebacterium pseudotuberculosis isolated from horses in California. In: Infection, Genetics and Evolution. 2017 ; Vol. 49. pp. 186-194.
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