Molecular epidemiology of Bartonella species isolated from ground squirrels and other rodents in northern California

A. C. Ziedins, Bruno B Chomel, R. W. Kasten, A. M. Kjemtrup, C. C. Chang

Research output: Contribution to journalArticle

Abstract

Bartonella spp. are endemic in wild rodents in many parts of the world. A study conducted in two northern California counties (Sonoma and Yolo) sampling California ground squirrels (Otospermophilus beecheyi) and four other rodent species (Peromyscus maniculatus, P. boylii, P.Truei and Neotoma fuscipes) led to the isolation of small Gram-negative bacilli which were identified as Bartonella spp. based on colony morphology, polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and partial gene sequencing. Overall, Bartonella spp. were isolated from the blood of 71% (32/45) of the ground squirrels and one third (22/66) of the other rodents. PCR-RFLP analysis of the gltA and 16S rRNA genes yielded seven unique profiles, four for the ground squirrels and three for the other rodents. Isolates from each PCR-RFLP profiles were submitted for partial sequencing. Ground squirrel isolates were most closely related to B. washoensis, whereas the other rodent isolates were closest to B. vinsonii subsp. vinsonii and B. vinsonii subsp. arupensis. Two of these three species or subspecies are known zoonotic agents.

Original languageEnglish (US)
Pages (from-to)1837-1844
Number of pages8
JournalEpidemiology and Infection
Volume144
Issue number9
DOIs
StatePublished - Jul 1 2016

Fingerprint

Bartonella
Sciuridae
Molecular Epidemiology
Rodentia
Restriction Fragment Length Polymorphisms
Polymerase Chain Reaction
Sigmodontinae
Peromyscus
Zoonoses
rRNA Genes
Bacillus
Genes

Keywords

  • Bacterial infections
  • Bartonella
  • deer mouse
  • dusky-footed woodrat
  • ground squirrel

ASJC Scopus subject areas

  • Infectious Diseases
  • Epidemiology

Cite this

Molecular epidemiology of Bartonella species isolated from ground squirrels and other rodents in northern California. / Ziedins, A. C.; Chomel, Bruno B; Kasten, R. W.; Kjemtrup, A. M.; Chang, C. C.

In: Epidemiology and Infection, Vol. 144, No. 9, 01.07.2016, p. 1837-1844.

Research output: Contribution to journalArticle

Ziedins, A. C. ; Chomel, Bruno B ; Kasten, R. W. ; Kjemtrup, A. M. ; Chang, C. C. / Molecular epidemiology of Bartonella species isolated from ground squirrels and other rodents in northern California. In: Epidemiology and Infection. 2016 ; Vol. 144, No. 9. pp. 1837-1844.
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