TY - JOUR
T1 - Molecular dosimetry of N 2-Hydroxymethyl-dG DNA adducts in rats exposed to formaldehyde
AU - Lu, Kun
AU - Moeller, Benjamin
AU - Doyle-Eisele, Melanie
AU - McDonald, Jacob
AU - Swenberg, James A.
PY - 2011/2/18
Y1 - 2011/2/18
N2 - In this study, both endogenous and exogenous N2-hydroxymethyl-dG adducts in nasal DNA of rats exposed to 0.7, 2, 5.8, 9.1, or 15.2 ppm [ 13CD2] formaldehyde for 6 h were quantified by a highly sensitive nano-UPLC-MS/MS method. Our data clearly demonstrated that exogenous formaldehyde DNA adducts form in a highly nonlinear fashion, with a 21.7-fold increase in exposure causing a 286-fold increase in exogenous adducts. The ratio of exogenous/endogenous DNA adducts demonstrated that endogenous DNA adducts dominated at low exposures, comprising more than 99%. In contrast, exogenous adducts were not detectable in the bone marrow of rats exposed to 15.2 ppm [13CD2] formaldehyde.
AB - In this study, both endogenous and exogenous N2-hydroxymethyl-dG adducts in nasal DNA of rats exposed to 0.7, 2, 5.8, 9.1, or 15.2 ppm [ 13CD2] formaldehyde for 6 h were quantified by a highly sensitive nano-UPLC-MS/MS method. Our data clearly demonstrated that exogenous formaldehyde DNA adducts form in a highly nonlinear fashion, with a 21.7-fold increase in exposure causing a 286-fold increase in exogenous adducts. The ratio of exogenous/endogenous DNA adducts demonstrated that endogenous DNA adducts dominated at low exposures, comprising more than 99%. In contrast, exogenous adducts were not detectable in the bone marrow of rats exposed to 15.2 ppm [13CD2] formaldehyde.
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U2 - 10.1021/tx1003886
DO - 10.1021/tx1003886
M3 - Article
C2 - 21155545
AN - SCOPUS:79951835425
VL - 24
SP - 159
EP - 161
JO - Chemical Research in Toxicology
JF - Chemical Research in Toxicology
SN - 0893-228X
IS - 2
ER -