Molecular diversity of the Trypanosoma cruzi TcSMUG family of mucin genes and proteins

Ivana Urban, Lucía Boiani Santurio, Agustina Chidichimo, Hai Yu, Xi Chen, Juan Mucci, Fernán Agüero, Carlos A. Buscaglia

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The surface of the protozoan Trypanosoma cruzi is covered by a dense coat of mucin-type glycoconjugates, which make a pivotal contribution to parasite protection and host immune evasion. Their importance is further underscored by the presence of >1000 mucin-like genes in the parasite genome. In the present study we demonstrate that one such group of genes, termed TcSMUG L, codes for previously unrecognized mucintype glycoconjugates anchored to and secreted from the surface of insect-dwelling epimastigotes. These features are supported by the in vivo tracing and characterization of endogenous TcSMUG L products and recombinant tagged molecules expressed by transfected parasites. Besides displaying substantial homology to TcSMUG S products, which provide the scaffold for the major Gp35/50 mucins also present in insect-dwelling stages of the T. cruzi lifecycle, TcSMUG L products display unique structural and functional features, including being completely refractory to sialylation by parasite trans-sialidases. Although quantitative real time-PCR and gene sequencing analyses indicate a high degree of genomic conservation across the T. cruzi species, TcSMUG L product expression and processing is quite variable among different parasite isolates.

Original languageEnglish (US)
Pages (from-to)303-313
Number of pages11
JournalBiochemical Journal
Volume438
Issue number2
DOIs
StatePublished - Sep 1 2011

Fingerprint

Trypanosoma cruzi
Mucins
Parasites
Genes
Glycoconjugates
Proteins
Insects
Immune Evasion
Scaffolds
Refractory materials
Real-Time Polymerase Chain Reaction
Conservation
Genome
Molecules
Processing

Keywords

  • Mucin
  • Multigene family
  • Quantitative PCR
  • TcSMUG
  • Trypanosoma cruzi

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Urban, I., Boiani Santurio, L., Chidichimo, A., Yu, H., Chen, X., Mucci, J., ... Buscaglia, C. A. (2011). Molecular diversity of the Trypanosoma cruzi TcSMUG family of mucin genes and proteins. Biochemical Journal, 438(2), 303-313. https://doi.org/10.1042/BJ20110683

Molecular diversity of the Trypanosoma cruzi TcSMUG family of mucin genes and proteins. / Urban, Ivana; Boiani Santurio, Lucía; Chidichimo, Agustina; Yu, Hai; Chen, Xi; Mucci, Juan; Agüero, Fernán; Buscaglia, Carlos A.

In: Biochemical Journal, Vol. 438, No. 2, 01.09.2011, p. 303-313.

Research output: Contribution to journalArticle

Urban, I, Boiani Santurio, L, Chidichimo, A, Yu, H, Chen, X, Mucci, J, Agüero, F & Buscaglia, CA 2011, 'Molecular diversity of the Trypanosoma cruzi TcSMUG family of mucin genes and proteins', Biochemical Journal, vol. 438, no. 2, pp. 303-313. https://doi.org/10.1042/BJ20110683
Urban, Ivana ; Boiani Santurio, Lucía ; Chidichimo, Agustina ; Yu, Hai ; Chen, Xi ; Mucci, Juan ; Agüero, Fernán ; Buscaglia, Carlos A. / Molecular diversity of the Trypanosoma cruzi TcSMUG family of mucin genes and proteins. In: Biochemical Journal. 2011 ; Vol. 438, No. 2. pp. 303-313.
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