Molecular diversity at the major cluster of disease resistance genes in cultivated and wild Lactuca spp.

D. Sicard, S. S. Woo, R. Arroyo-Garcia, O. Ochoa, D. Nguyen, A. Korol, E. Nevo, Richard W Michelmore

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Diversity was analyzed in wild and cultivated Lactuca germplasm using molecular markers derived from resistance genes of the NBS-LRR type. Three molecular markers, one microsatellite marker and two SCAR markers that amplified LRR-encoding regions, were developed from sequences of resistance gene homologs at the main resistance gene cluster in lettuce. Variation for these markers were assessed in germplasm including accessions of cultivated lettuce, Lactuca sativa L. and three wild Lactuca spp., L. serriola L., L. saligna and L. virosa L. Diversity was also studied within and between natural populations of L. serriola from Israel and California; the former is close to the center of diversity for Lactuca spp. while the latter is an area of more recent colonization. Large numbers of haplotypes were detected indicating the presence of numerous resistance genes in wild species. The diversity in haplotypes provided evidence for gene duplication and unequal crossing-over during the evolution of this cluster of resistance genes. However, there was no evidence for duplications and deletions within the LRR-encoding regions studied. The three markers were highly correlated with resistance phenotypes in L. sativa. They were able to discriminate between accessions that had previously been shown to be resistant to all known isolates of Bremia lactucae. Therefore, these markers will be highly informative for the establishment of core collections and marker-aided selection. A hierarchical analysis of the population structure of L. serriola showed that countries, as well as locations, were significantly differentiated. These differences may reflect local founder effects and/or divergent selection.

Original languageEnglish (US)
Pages (from-to)405-418
Number of pages14
JournalTheoretical And Applied Genetics
Volume99
Issue number3-4
DOIs
StatePublished - 1999

Fingerprint

Lactuca
Lettuce
Disease Resistance
disease resistance
Lactuca serriola
Lactuca sativa
Multigene Family
lettuce
Haplotypes
Genes
haplotypes
germplasm
Lactuca virosa
Lactuca saligna
genes
Bremia lactucae
Founder Effect
center of diversity
genetic markers
Gene Duplication

Keywords

  • Diversity
  • Lactuca
  • LRR multigene family
  • Microsatellite
  • Resistance genes

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Horticulture
  • Plant Science
  • Genetics
  • Genetics(clinical)

Cite this

Sicard, D., Woo, S. S., Arroyo-Garcia, R., Ochoa, O., Nguyen, D., Korol, A., ... Michelmore, R. W. (1999). Molecular diversity at the major cluster of disease resistance genes in cultivated and wild Lactuca spp. Theoretical And Applied Genetics, 99(3-4), 405-418. https://doi.org/10.1007/s001220051251

Molecular diversity at the major cluster of disease resistance genes in cultivated and wild Lactuca spp. / Sicard, D.; Woo, S. S.; Arroyo-Garcia, R.; Ochoa, O.; Nguyen, D.; Korol, A.; Nevo, E.; Michelmore, Richard W.

In: Theoretical And Applied Genetics, Vol. 99, No. 3-4, 1999, p. 405-418.

Research output: Contribution to journalArticle

Sicard, D, Woo, SS, Arroyo-Garcia, R, Ochoa, O, Nguyen, D, Korol, A, Nevo, E & Michelmore, RW 1999, 'Molecular diversity at the major cluster of disease resistance genes in cultivated and wild Lactuca spp.', Theoretical And Applied Genetics, vol. 99, no. 3-4, pp. 405-418. https://doi.org/10.1007/s001220051251
Sicard, D. ; Woo, S. S. ; Arroyo-Garcia, R. ; Ochoa, O. ; Nguyen, D. ; Korol, A. ; Nevo, E. ; Michelmore, Richard W. / Molecular diversity at the major cluster of disease resistance genes in cultivated and wild Lactuca spp. In: Theoretical And Applied Genetics. 1999 ; Vol. 99, No. 3-4. pp. 405-418.
@article{1cdf0f68bb7d43f8951eab7a4e250c13,
title = "Molecular diversity at the major cluster of disease resistance genes in cultivated and wild Lactuca spp.",
abstract = "Diversity was analyzed in wild and cultivated Lactuca germplasm using molecular markers derived from resistance genes of the NBS-LRR type. Three molecular markers, one microsatellite marker and two SCAR markers that amplified LRR-encoding regions, were developed from sequences of resistance gene homologs at the main resistance gene cluster in lettuce. Variation for these markers were assessed in germplasm including accessions of cultivated lettuce, Lactuca sativa L. and three wild Lactuca spp., L. serriola L., L. saligna and L. virosa L. Diversity was also studied within and between natural populations of L. serriola from Israel and California; the former is close to the center of diversity for Lactuca spp. while the latter is an area of more recent colonization. Large numbers of haplotypes were detected indicating the presence of numerous resistance genes in wild species. The diversity in haplotypes provided evidence for gene duplication and unequal crossing-over during the evolution of this cluster of resistance genes. However, there was no evidence for duplications and deletions within the LRR-encoding regions studied. The three markers were highly correlated with resistance phenotypes in L. sativa. They were able to discriminate between accessions that had previously been shown to be resistant to all known isolates of Bremia lactucae. Therefore, these markers will be highly informative for the establishment of core collections and marker-aided selection. A hierarchical analysis of the population structure of L. serriola showed that countries, as well as locations, were significantly differentiated. These differences may reflect local founder effects and/or divergent selection.",
keywords = "Diversity, Lactuca, LRR multigene family, Microsatellite, Resistance genes",
author = "D. Sicard and Woo, {S. S.} and R. Arroyo-Garcia and O. Ochoa and D. Nguyen and A. Korol and E. Nevo and Michelmore, {Richard W}",
year = "1999",
doi = "10.1007/s001220051251",
language = "English (US)",
volume = "99",
pages = "405--418",
journal = "Theoretical And Applied Genetics",
issn = "0040-5752",
publisher = "Springer Verlag",
number = "3-4",

}

TY - JOUR

T1 - Molecular diversity at the major cluster of disease resistance genes in cultivated and wild Lactuca spp.

AU - Sicard, D.

AU - Woo, S. S.

AU - Arroyo-Garcia, R.

AU - Ochoa, O.

AU - Nguyen, D.

AU - Korol, A.

AU - Nevo, E.

AU - Michelmore, Richard W

PY - 1999

Y1 - 1999

N2 - Diversity was analyzed in wild and cultivated Lactuca germplasm using molecular markers derived from resistance genes of the NBS-LRR type. Three molecular markers, one microsatellite marker and two SCAR markers that amplified LRR-encoding regions, were developed from sequences of resistance gene homologs at the main resistance gene cluster in lettuce. Variation for these markers were assessed in germplasm including accessions of cultivated lettuce, Lactuca sativa L. and three wild Lactuca spp., L. serriola L., L. saligna and L. virosa L. Diversity was also studied within and between natural populations of L. serriola from Israel and California; the former is close to the center of diversity for Lactuca spp. while the latter is an area of more recent colonization. Large numbers of haplotypes were detected indicating the presence of numerous resistance genes in wild species. The diversity in haplotypes provided evidence for gene duplication and unequal crossing-over during the evolution of this cluster of resistance genes. However, there was no evidence for duplications and deletions within the LRR-encoding regions studied. The three markers were highly correlated with resistance phenotypes in L. sativa. They were able to discriminate between accessions that had previously been shown to be resistant to all known isolates of Bremia lactucae. Therefore, these markers will be highly informative for the establishment of core collections and marker-aided selection. A hierarchical analysis of the population structure of L. serriola showed that countries, as well as locations, were significantly differentiated. These differences may reflect local founder effects and/or divergent selection.

AB - Diversity was analyzed in wild and cultivated Lactuca germplasm using molecular markers derived from resistance genes of the NBS-LRR type. Three molecular markers, one microsatellite marker and two SCAR markers that amplified LRR-encoding regions, were developed from sequences of resistance gene homologs at the main resistance gene cluster in lettuce. Variation for these markers were assessed in germplasm including accessions of cultivated lettuce, Lactuca sativa L. and three wild Lactuca spp., L. serriola L., L. saligna and L. virosa L. Diversity was also studied within and between natural populations of L. serriola from Israel and California; the former is close to the center of diversity for Lactuca spp. while the latter is an area of more recent colonization. Large numbers of haplotypes were detected indicating the presence of numerous resistance genes in wild species. The diversity in haplotypes provided evidence for gene duplication and unequal crossing-over during the evolution of this cluster of resistance genes. However, there was no evidence for duplications and deletions within the LRR-encoding regions studied. The three markers were highly correlated with resistance phenotypes in L. sativa. They were able to discriminate between accessions that had previously been shown to be resistant to all known isolates of Bremia lactucae. Therefore, these markers will be highly informative for the establishment of core collections and marker-aided selection. A hierarchical analysis of the population structure of L. serriola showed that countries, as well as locations, were significantly differentiated. These differences may reflect local founder effects and/or divergent selection.

KW - Diversity

KW - Lactuca

KW - LRR multigene family

KW - Microsatellite

KW - Resistance genes

UR - http://www.scopus.com/inward/record.url?scp=0032883573&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032883573&partnerID=8YFLogxK

U2 - 10.1007/s001220051251

DO - 10.1007/s001220051251

M3 - Article

C2 - 22665172

AN - SCOPUS:0032883573

VL - 99

SP - 405

EP - 418

JO - Theoretical And Applied Genetics

JF - Theoretical And Applied Genetics

SN - 0040-5752

IS - 3-4

ER -