Molecular detection of Streptococcus equi subspecies equi in face flies (Musca autumnalis) collected during a strangles outbreak on a Thoroughbred farm

Nicola Pusterla, J. Bowers, S. Barnum, J. A. Hall

Research output: Contribution to journalArticle

Abstract

The objective of this study was to detect Streptococcus equi subspecies equi (S. equi) (Lactobacillales: Streptococcaceae) using quantitative polymerase chain reaction (qPCR) in flies collected from a farm with a documented outbreak of strangles. A total of 1856 face flies [Musca autumnalis (Diptera: Muscidae)] were collected using conventional fly traps. The flies were processed for nucleic acid purification and tested for the presence of S. equi by qPCR. A total of 10/1856 flies (0.54%) tested qPCR-positive for S. equi. The results may implicate the presence of face flies as a risk factor for the transmission of S. equi and highlight the need to institute proper husbandry measures, biosecurity protocols and fly control in order to reduce the potential for infection in at-risk horses.

Original languageEnglish (US)
JournalMedical and Veterinary Entomology
DOIs
StatePublished - Jan 1 2019

Keywords

  • Face fly
  • qPCR
  • risk factor
  • Streptococcus equi subspecies equi

ASJC Scopus subject areas

  • Parasitology
  • Ecology, Evolution, Behavior and Systematics
  • veterinary(all)
  • Insect Science

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