Molecular detection of Streptococcus equi subspecies equi in face flies (Musca autumnalis) collected during a strangles outbreak on a Thoroughbred farm

Nicola Pusterla, J. Bowers, S. Barnum, J. A. Hall

Research output: Contribution to journalArticle

Abstract

The objective of this study was to detect Streptococcus equi subspecies equi (S. equi) (Lactobacillales: Streptococcaceae) using quantitative polymerase chain reaction (qPCR) in flies collected from a farm with a documented outbreak of strangles. A total of 1856 face flies [Musca autumnalis (Diptera: Muscidae)] were collected using conventional fly traps. The flies were processed for nucleic acid purification and tested for the presence of S. equi by qPCR. A total of 10/1856 flies (0.54%) tested qPCR-positive for S. equi. The results may implicate the presence of face flies as a risk factor for the transmission of S. equi and highlight the need to institute proper husbandry measures, biosecurity protocols and fly control in order to reduce the potential for infection in at-risk horses.

Original languageEnglish (US)
JournalMedical and Veterinary Entomology
DOIs
StatePublished - Jan 1 2019

Fingerprint

Streptococcus equi
strangles
Muscidae
Musca autumnalis
Equus
Diptera
subspecies
Disease Outbreaks
farm
farms
polymerase chain reaction
quantitative polymerase chain reaction
Polymerase Chain Reaction
Lactobacillales
Streptococcaceae
biosecurity
nucleic acid
horse
risk factor
Nucleic Acids

Keywords

  • Face fly
  • qPCR
  • risk factor
  • Streptococcus equi subspecies equi

ASJC Scopus subject areas

  • Parasitology
  • Ecology, Evolution, Behavior and Systematics
  • veterinary(all)
  • Insect Science

Cite this

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title = "Molecular detection of Streptococcus equi subspecies equi in face flies (Musca autumnalis) collected during a strangles outbreak on a Thoroughbred farm",
abstract = "The objective of this study was to detect Streptococcus equi subspecies equi (S. equi) (Lactobacillales: Streptococcaceae) using quantitative polymerase chain reaction (qPCR) in flies collected from a farm with a documented outbreak of strangles. A total of 1856 face flies [Musca autumnalis (Diptera: Muscidae)] were collected using conventional fly traps. The flies were processed for nucleic acid purification and tested for the presence of S. equi by qPCR. A total of 10/1856 flies (0.54{\%}) tested qPCR-positive for S. equi. The results may implicate the presence of face flies as a risk factor for the transmission of S. equi and highlight the need to institute proper husbandry measures, biosecurity protocols and fly control in order to reduce the potential for infection in at-risk horses.",
keywords = "Face fly, qPCR, risk factor, Streptococcus equi subspecies equi",
author = "Nicola Pusterla and J. Bowers and S. Barnum and Hall, {J. A.}",
year = "2019",
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AU - Pusterla, Nicola

AU - Bowers, J.

AU - Barnum, S.

AU - Hall, J. A.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - The objective of this study was to detect Streptococcus equi subspecies equi (S. equi) (Lactobacillales: Streptococcaceae) using quantitative polymerase chain reaction (qPCR) in flies collected from a farm with a documented outbreak of strangles. A total of 1856 face flies [Musca autumnalis (Diptera: Muscidae)] were collected using conventional fly traps. The flies were processed for nucleic acid purification and tested for the presence of S. equi by qPCR. A total of 10/1856 flies (0.54%) tested qPCR-positive for S. equi. The results may implicate the presence of face flies as a risk factor for the transmission of S. equi and highlight the need to institute proper husbandry measures, biosecurity protocols and fly control in order to reduce the potential for infection in at-risk horses.

AB - The objective of this study was to detect Streptococcus equi subspecies equi (S. equi) (Lactobacillales: Streptococcaceae) using quantitative polymerase chain reaction (qPCR) in flies collected from a farm with a documented outbreak of strangles. A total of 1856 face flies [Musca autumnalis (Diptera: Muscidae)] were collected using conventional fly traps. The flies were processed for nucleic acid purification and tested for the presence of S. equi by qPCR. A total of 10/1856 flies (0.54%) tested qPCR-positive for S. equi. The results may implicate the presence of face flies as a risk factor for the transmission of S. equi and highlight the need to institute proper husbandry measures, biosecurity protocols and fly control in order to reduce the potential for infection in at-risk horses.

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KW - qPCR

KW - risk factor

KW - Streptococcus equi subspecies equi

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