Molecular detection of microbes in nasal tissue of dogs with idiopathic lymphoplasmacytic rhinitis

Rebecca C. Windsor, Lynelle R Johnson, Jane E Sykes, Tracy L. Drazenovich, Christian M. Leutenegger, Hilde E V De Cock

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Lymphoplasmacytic rhinitis (LPR) is a common histologic finding in dogs with chronic nasal disease; however, potential etiologies of this disorder have not been examined. We investigated the hypothesis that specific microbes contribute to clinical disease in dogs with LPR. Paraffin-embedded nasal biopsies were obtained from 19 dogs with LPR, 10 dogs with nasal neoplasia, and 10 dogs with nasal aspergillosis. Nucleic acids were extracted from paraffin blocks, and real-time quantitative polymerase chain reaction (PCR) was employed for detection of target genes for bacterial and fungal DNA, canine adenovirus 2 (CAV-2), parainfluenza virus 3 (PI-3), Chlamydia/Chlamydophila spp., and Bartonella spp. Conventional PCR was used for detection of Mycoplasma spp. Statistical analysis was performed using the Mann-Whitney U-test for nonparametric data, and significance was set at P < 0.05. DNA or RNA for CAV-2, PI-3, Bartonella, Mycoplasma, and Chlamydophila was not detected in any nasal biopsy. DNA loads for bacterial DNA did not differ among disease groups. Detection of fungal DNA in nasal biopsies was highest in dogs with aspergillosis (P < 0.0001); however, nasal biopsies of LPR dogs also displayed higher fungal DNA levels than samples from dogs with nasal neoplasia (P = 0.016). Detection of high levels of fungal DNA in nasal biopsies of dogs with LPR suggests that fungal organisms may be causally associated with the inflammation observed, although the possibility of entrapment or accumulation of fungi in the nasal cavity due to chronic inflammation cannot be excluded. Further investigations are required to elucidate the underlying etiopathogenesis of LPR.

Original languageEnglish (US)
Pages (from-to)250-256
Number of pages7
JournalJournal of Veterinary Internal Medicine
Volume20
Issue number2
DOIs
StatePublished - Mar 2006

Fingerprint

rhinitis
Rhinitis
Nose
Fungal DNA
Dogs
microorganisms
dogs
biopsy
DNA
Biopsy
Canine Adenoviruses
Chlamydophila
Bartonella
Paramyxoviridae Infections
Canine adenovirus A
Bacterial DNA
Aspergillosis
Mycoplasma
aspergillosis
Paraffin

Keywords

  • Gene
  • Inflammation
  • Nucleic acid
  • Polymerase chain reaction

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Molecular detection of microbes in nasal tissue of dogs with idiopathic lymphoplasmacytic rhinitis. / Windsor, Rebecca C.; Johnson, Lynelle R; Sykes, Jane E; Drazenovich, Tracy L.; Leutenegger, Christian M.; De Cock, Hilde E V.

In: Journal of Veterinary Internal Medicine, Vol. 20, No. 2, 03.2006, p. 250-256.

Research output: Contribution to journalArticle

Windsor, Rebecca C. ; Johnson, Lynelle R ; Sykes, Jane E ; Drazenovich, Tracy L. ; Leutenegger, Christian M. ; De Cock, Hilde E V. / Molecular detection of microbes in nasal tissue of dogs with idiopathic lymphoplasmacytic rhinitis. In: Journal of Veterinary Internal Medicine. 2006 ; Vol. 20, No. 2. pp. 250-256.
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abstract = "Lymphoplasmacytic rhinitis (LPR) is a common histologic finding in dogs with chronic nasal disease; however, potential etiologies of this disorder have not been examined. We investigated the hypothesis that specific microbes contribute to clinical disease in dogs with LPR. Paraffin-embedded nasal biopsies were obtained from 19 dogs with LPR, 10 dogs with nasal neoplasia, and 10 dogs with nasal aspergillosis. Nucleic acids were extracted from paraffin blocks, and real-time quantitative polymerase chain reaction (PCR) was employed for detection of target genes for bacterial and fungal DNA, canine adenovirus 2 (CAV-2), parainfluenza virus 3 (PI-3), Chlamydia/Chlamydophila spp., and Bartonella spp. Conventional PCR was used for detection of Mycoplasma spp. Statistical analysis was performed using the Mann-Whitney U-test for nonparametric data, and significance was set at P < 0.05. DNA or RNA for CAV-2, PI-3, Bartonella, Mycoplasma, and Chlamydophila was not detected in any nasal biopsy. DNA loads for bacterial DNA did not differ among disease groups. Detection of fungal DNA in nasal biopsies was highest in dogs with aspergillosis (P < 0.0001); however, nasal biopsies of LPR dogs also displayed higher fungal DNA levels than samples from dogs with nasal neoplasia (P = 0.016). Detection of high levels of fungal DNA in nasal biopsies of dogs with LPR suggests that fungal organisms may be causally associated with the inflammation observed, although the possibility of entrapment or accumulation of fungi in the nasal cavity due to chronic inflammation cannot be excluded. Further investigations are required to elucidate the underlying etiopathogenesis of LPR.",
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