Molecular detection of an Ehrlichia-like agent in rainbow trout (Oncorhynchus mykiss) from Northern California

Nicola Pusterla, Eileen Johnson, Joon Seok Chae, Elfriede Derock, Mel Willis, Ronald Hedrick, John E Madigan

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Ehrlichia DNA was identified by nested PCR in rainbow trout (Oncorhynchus mykiss) collected from a creek in northern California where Potomac horse fever is endemic. Ehrlichia DNA was found in tissues from several organs including the gills, heart, spleen, liver, kidneys and intestine of trout and from three different adult digenetic trematodes (Deropegus sp., Crepidostomum sp., Creptotrema sp.) parasitizing the gallbladder and/or the intestine of the trout. Sequencing of PCR-amplified DNA from the 16S rRNA gene indicated that the source organism was most closely related to the sequences of E. risticii (level of sequence similarity 96.0%), the SF agent (95.9%), E. sennetsu (95.8%), and Neorickettsia helminthoeca (95.3%). The data suggest that trout and parasitic trematodes may be involved in the epidemiology of an Ehrlichia-like agent belonging to the E. sennetsu genogroup. Whether the fish agent infects horses, dogs, or human beings, and whether it causes disease, remain to be determined. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)199-207
Number of pages9
JournalVeterinary Parasitology
Volume92
Issue number3
DOIs
StatePublished - Oct 1 2000

Fingerprint

Ehrlichia
Trout
Oncorhynchus mykiss
trout
Trematoda
Neorickettsia
Horses
Neorickettsia helminthoeca
Intestines
DNA
intestines
Crepidostomum
Potomac horse fever
Polymerase Chain Reaction
gall bladder
Gallbladder
rRNA Genes
epidemiology
gills
Fishes

Keywords

  • Digenetic trematodes
  • Ehrlichia-like agent
  • Molecular detection
  • Rainbow trout

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Parasitology
  • veterinary(all)

Cite this

Molecular detection of an Ehrlichia-like agent in rainbow trout (Oncorhynchus mykiss) from Northern California. / Pusterla, Nicola; Johnson, Eileen; Chae, Joon Seok; Derock, Elfriede; Willis, Mel; Hedrick, Ronald; Madigan, John E.

In: Veterinary Parasitology, Vol. 92, No. 3, 01.10.2000, p. 199-207.

Research output: Contribution to journalArticle

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