Abstract
Ehrlichia DNA was identified by nested PCR in rainbow trout (Oncorhynchus mykiss) collected from a creek in northern California where Potomac horse fever is endemic. Ehrlichia DNA was found in tissues from several organs including the gills, heart, spleen, liver, kidneys and intestine of trout and from three different adult digenetic trematodes (Deropegus sp., Crepidostomum sp., Creptotrema sp.) parasitizing the gallbladder and/or the intestine of the trout. Sequencing of PCR-amplified DNA from the 16S rRNA gene indicated that the source organism was most closely related to the sequences of E. risticii (level of sequence similarity 96.0%), the SF agent (95.9%), E. sennetsu (95.8%), and Neorickettsia helminthoeca (95.3%). The data suggest that trout and parasitic trematodes may be involved in the epidemiology of an Ehrlichia-like agent belonging to the E. sennetsu genogroup. Whether the fish agent infects horses, dogs, or human beings, and whether it causes disease, remain to be determined. Copyright (C) 2000 Elsevier Science B.V.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 199-207 |
| Number of pages | 9 |
| Journal | Veterinary Parasitology |
| Volume | 92 |
| Issue number | 3 |
| DOIs | |
| State | Published - Oct 1 2000 |
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Keywords
- Digenetic trematodes
- Ehrlichia-like agent
- Molecular detection
- Rainbow trout
ASJC Scopus subject areas
- Animal Science and Zoology
- Parasitology
- veterinary(all)
Cite this
Molecular detection of an Ehrlichia-like agent in rainbow trout (Oncorhynchus mykiss) from Northern California. / Pusterla, Nicola; Johnson, Eileen; Chae, Joon Seok; Derock, Elfriede; Willis, Mel; Hedrick, Ronald; Madigan, John E.
In: Veterinary Parasitology, Vol. 92, No. 3, 01.10.2000, p. 199-207.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular detection of an Ehrlichia-like agent in rainbow trout (Oncorhynchus mykiss) from Northern California
AU - Pusterla, Nicola
AU - Johnson, Eileen
AU - Chae, Joon Seok
AU - Derock, Elfriede
AU - Willis, Mel
AU - Hedrick, Ronald
AU - Madigan, John E
PY - 2000/10/1
Y1 - 2000/10/1
N2 - Ehrlichia DNA was identified by nested PCR in rainbow trout (Oncorhynchus mykiss) collected from a creek in northern California where Potomac horse fever is endemic. Ehrlichia DNA was found in tissues from several organs including the gills, heart, spleen, liver, kidneys and intestine of trout and from three different adult digenetic trematodes (Deropegus sp., Crepidostomum sp., Creptotrema sp.) parasitizing the gallbladder and/or the intestine of the trout. Sequencing of PCR-amplified DNA from the 16S rRNA gene indicated that the source organism was most closely related to the sequences of E. risticii (level of sequence similarity 96.0%), the SF agent (95.9%), E. sennetsu (95.8%), and Neorickettsia helminthoeca (95.3%). The data suggest that trout and parasitic trematodes may be involved in the epidemiology of an Ehrlichia-like agent belonging to the E. sennetsu genogroup. Whether the fish agent infects horses, dogs, or human beings, and whether it causes disease, remain to be determined. Copyright (C) 2000 Elsevier Science B.V.
AB - Ehrlichia DNA was identified by nested PCR in rainbow trout (Oncorhynchus mykiss) collected from a creek in northern California where Potomac horse fever is endemic. Ehrlichia DNA was found in tissues from several organs including the gills, heart, spleen, liver, kidneys and intestine of trout and from three different adult digenetic trematodes (Deropegus sp., Crepidostomum sp., Creptotrema sp.) parasitizing the gallbladder and/or the intestine of the trout. Sequencing of PCR-amplified DNA from the 16S rRNA gene indicated that the source organism was most closely related to the sequences of E. risticii (level of sequence similarity 96.0%), the SF agent (95.9%), E. sennetsu (95.8%), and Neorickettsia helminthoeca (95.3%). The data suggest that trout and parasitic trematodes may be involved in the epidemiology of an Ehrlichia-like agent belonging to the E. sennetsu genogroup. Whether the fish agent infects horses, dogs, or human beings, and whether it causes disease, remain to be determined. Copyright (C) 2000 Elsevier Science B.V.
KW - Digenetic trematodes
KW - Ehrlichia-like agent
KW - Molecular detection
KW - Rainbow trout
UR - http://www.scopus.com/inward/record.url?scp=0034308242&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034308242&partnerID=8YFLogxK
U2 - 10.1016/S0304-4017(00)00287-9
DO - 10.1016/S0304-4017(00)00287-9
M3 - Article
C2 - 10962157
AN - SCOPUS:0034308242
VL - 92
SP - 199
EP - 207
JO - Veterinary Parasitology: Regional Studies and Reports
JF - Veterinary Parasitology: Regional Studies and Reports
SN - 0304-4017
IS - 3
ER -