Molecular detection and phylogenetic placement of a microsporidian from English sole (Pleuronectes vetulus) affected by X-cell pseudotumors

J. S. Khattra, S. J. Gresoviac, M. L. Kent, M. S. Myers, Ronald Hedrick, R. H. Devlin

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Flatfish tissue samples exhibiting X-cell pseudotumors were tested with a number of ribosomal DNA (rDNA) general primers in polymerase chain reactions (PCRs). Microsporidian primers resulted in the amplification of an rDNA fragment and molecular phylogenetic analysis indicated that although the organism did not relate closely with any current microsporidian genera, it was most similar to Nucleospora salmonis and branched within the Enterocytozoonidae. Re-examination of the original tissues used for DNA extractions revealed the presence of putative microsporidian spores in PCR-positive samples. These observations reiterate the highly sensitive diagnostic feature of PCR, allowing detection of organisms overlooked by conventional methods and demonstrate the occurrence of rare, coinfecting organisms.

Original languageEnglish (US)
Pages (from-to)867-871
Number of pages5
JournalJournal of Parasitology
Volume86
Issue number4
StatePublished - Sep 4 2000
Externally publishedYes

Fingerprint

Parophrys vetulus
Flounder
Microsporidia
polymerase chain reaction
Ribosomal DNA
phylogenetics
DNA
ribosomal DNA
Polymerase Chain Reaction
phylogeny
organisms
Flatfishes
flatfish
DNA Primers
Pleuronectiformes
cells
Spores
spore
amplification
spores

ASJC Scopus subject areas

  • Parasitology
  • Medicine(all)
  • Ecology, Evolution, Behavior and Systematics

Cite this

Molecular detection and phylogenetic placement of a microsporidian from English sole (Pleuronectes vetulus) affected by X-cell pseudotumors. / Khattra, J. S.; Gresoviac, S. J.; Kent, M. L.; Myers, M. S.; Hedrick, Ronald; Devlin, R. H.

In: Journal of Parasitology, Vol. 86, No. 4, 04.09.2000, p. 867-871.

Research output: Contribution to journalArticle

Khattra, J. S. ; Gresoviac, S. J. ; Kent, M. L. ; Myers, M. S. ; Hedrick, Ronald ; Devlin, R. H. / Molecular detection and phylogenetic placement of a microsporidian from English sole (Pleuronectes vetulus) affected by X-cell pseudotumors. In: Journal of Parasitology. 2000 ; Vol. 86, No. 4. pp. 867-871.
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