Molecular correlates of laminar differences in the macaque dorsal lateral geniculate nucleus

Karl D Murray, Carol M. Rubin, Edward G. Jones, Leo M. Chalupa

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

In anthropoid primates, cells in the magnocellular and parvocellular layers of the dorsal lateral geniculate nucleus (dLGN) are distinguished by unique retinal inputs, receptive field properties, and laminar terminations of their axons in visual cortex. To identify genes underlying these phenotypic differences, we screened RNA from magnocellular and parvocellular layers of adult macaque dLGN for layer-specific differences in gene expression. Real-time quantitative reverse transcription-PCR and in situ hybridization were used to confirm gene expression in adult and fetal macaque. Cellular localization of gene expression revealed 11 new layer-specific markers, of which 10 were enriched in magnocellular layers (BRD4, CAV1, EEF1A2, FAM108A1, INα, KCNA1, NEFH, NEFL, PPP2R2C, and SFRP2) and one was enriched in parvocellular and koniocellular layers (TCF7L2). These markers relate to functions involved in development, transcription, and cell signaling, with Wnt/β-catenin and neurofilament pathways figuring prominently. A subset of markers was differentially expressed in the fetal dLGN during a developmental epoch critical for magnocellular and parvocellular pathway formation. These results provide new evidence for the molecular differentiation of magnocellular and parvocellular streams through the primate dLGN.

Original languageEnglish (US)
Pages (from-to)12010-12022
Number of pages13
JournalJournal of Neuroscience
Volume28
Issue number46
DOIs
StatePublished - Nov 12 2008

Fingerprint

Geniculate Bodies
Macaca
Gene Expression
Primates
Catenins
Intermediate Filaments
Visual Cortex
Reverse Transcription
Haplorhini
In Situ Hybridization
Axons
RNA
Polymerase Chain Reaction
Genes

Keywords

  • Axon targeting
  • Cell signaling
  • Cytoarchitecture
  • Plasticity
  • Transcriptome
  • Vision

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Molecular correlates of laminar differences in the macaque dorsal lateral geniculate nucleus. / Murray, Karl D; Rubin, Carol M.; Jones, Edward G.; Chalupa, Leo M.

In: Journal of Neuroscience, Vol. 28, No. 46, 12.11.2008, p. 12010-12022.

Research output: Contribution to journalArticle

Murray, Karl D ; Rubin, Carol M. ; Jones, Edward G. ; Chalupa, Leo M. / Molecular correlates of laminar differences in the macaque dorsal lateral geniculate nucleus. In: Journal of Neuroscience. 2008 ; Vol. 28, No. 46. pp. 12010-12022.
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