Abstract
In anthropoid primates, cells in the magnocellular and parvocellular layers of the dorsal lateral geniculate nucleus (dLGN) are distinguished by unique retinal inputs, receptive field properties, and laminar terminations of their axons in visual cortex. To identify genes underlying these phenotypic differences, we screened RNA from magnocellular and parvocellular layers of adult macaque dLGN for layer-specific differences in gene expression. Real-time quantitative reverse transcription-PCR and in situ hybridization were used to confirm gene expression in adult and fetal macaque. Cellular localization of gene expression revealed 11 new layer-specific markers, of which 10 were enriched in magnocellular layers (BRD4, CAV1, EEF1A2, FAM108A1, INα, KCNA1, NEFH, NEFL, PPP2R2C, and SFRP2) and one was enriched in parvocellular and koniocellular layers (TCF7L2). These markers relate to functions involved in development, transcription, and cell signaling, with Wnt/β-catenin and neurofilament pathways figuring prominently. A subset of markers was differentially expressed in the fetal dLGN during a developmental epoch critical for magnocellular and parvocellular pathway formation. These results provide new evidence for the molecular differentiation of magnocellular and parvocellular streams through the primate dLGN.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 12010-12022 |
| Number of pages | 13 |
| Journal | Journal of Neuroscience |
| Volume | 28 |
| Issue number | 46 |
| DOIs | |
| State | Published - Nov 12 2008 |
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Keywords
- Axon targeting
- Cell signaling
- Cytoarchitecture
- Plasticity
- Transcriptome
- Vision
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Molecular correlates of laminar differences in the macaque dorsal lateral geniculate nucleus. / Murray, Karl D; Rubin, Carol M.; Jones, Edward G.; Chalupa, Leo M.
In: Journal of Neuroscience, Vol. 28, No. 46, 12.11.2008, p. 12010-12022.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular correlates of laminar differences in the macaque dorsal lateral geniculate nucleus
AU - Murray, Karl D
AU - Rubin, Carol M.
AU - Jones, Edward G.
AU - Chalupa, Leo M.
PY - 2008/11/12
Y1 - 2008/11/12
N2 - In anthropoid primates, cells in the magnocellular and parvocellular layers of the dorsal lateral geniculate nucleus (dLGN) are distinguished by unique retinal inputs, receptive field properties, and laminar terminations of their axons in visual cortex. To identify genes underlying these phenotypic differences, we screened RNA from magnocellular and parvocellular layers of adult macaque dLGN for layer-specific differences in gene expression. Real-time quantitative reverse transcription-PCR and in situ hybridization were used to confirm gene expression in adult and fetal macaque. Cellular localization of gene expression revealed 11 new layer-specific markers, of which 10 were enriched in magnocellular layers (BRD4, CAV1, EEF1A2, FAM108A1, INα, KCNA1, NEFH, NEFL, PPP2R2C, and SFRP2) and one was enriched in parvocellular and koniocellular layers (TCF7L2). These markers relate to functions involved in development, transcription, and cell signaling, with Wnt/β-catenin and neurofilament pathways figuring prominently. A subset of markers was differentially expressed in the fetal dLGN during a developmental epoch critical for magnocellular and parvocellular pathway formation. These results provide new evidence for the molecular differentiation of magnocellular and parvocellular streams through the primate dLGN.
AB - In anthropoid primates, cells in the magnocellular and parvocellular layers of the dorsal lateral geniculate nucleus (dLGN) are distinguished by unique retinal inputs, receptive field properties, and laminar terminations of their axons in visual cortex. To identify genes underlying these phenotypic differences, we screened RNA from magnocellular and parvocellular layers of adult macaque dLGN for layer-specific differences in gene expression. Real-time quantitative reverse transcription-PCR and in situ hybridization were used to confirm gene expression in adult and fetal macaque. Cellular localization of gene expression revealed 11 new layer-specific markers, of which 10 were enriched in magnocellular layers (BRD4, CAV1, EEF1A2, FAM108A1, INα, KCNA1, NEFH, NEFL, PPP2R2C, and SFRP2) and one was enriched in parvocellular and koniocellular layers (TCF7L2). These markers relate to functions involved in development, transcription, and cell signaling, with Wnt/β-catenin and neurofilament pathways figuring prominently. A subset of markers was differentially expressed in the fetal dLGN during a developmental epoch critical for magnocellular and parvocellular pathway formation. These results provide new evidence for the molecular differentiation of magnocellular and parvocellular streams through the primate dLGN.
KW - Axon targeting
KW - Cell signaling
KW - Cytoarchitecture
KW - Plasticity
KW - Transcriptome
KW - Vision
UR - http://www.scopus.com/inward/record.url?scp=58149333213&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=58149333213&partnerID=8YFLogxK
U2 - 10.1523/JNEUROSCI.3800-08.2008
DO - 10.1523/JNEUROSCI.3800-08.2008
M3 - Article
C2 - 19005066
AN - SCOPUS:58149333213
VL - 28
SP - 12010
EP - 12022
JO - Journal of Neuroscience
JF - Journal of Neuroscience
SN - 0270-6474
IS - 46
ER -