Molecular cloning of transmembrane and soluble forms of a novel rat natural killer cell receptor related to 2B4

Pappanaicken R. Kumaresan, Susan E. Stepp, Michael Bennett, Vinay Kumar, Porunelloor A. Mathew

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Natural killer (NK)-cell recognition of target cells and cytolytic function are controlled by multiple receptor-ligand interactions. These receptors can transmit either positive or negative signals and belong to the lectin superfamily or immunoglobulin superfamily (IgSF). One member of the IgSF, 2B4, is expressed on the surface of all mouse and human NK cells and the subset of T cells that mediate NK-like killing. In both mouse and human, 2B4 is a transmembrane protein and is the counter-receptor for CD48. Northern blot analysis had indicated the existence of 2B4-related genes. Here we report the cloning of novel cDNAs (r2B4R) closely related to the rat 2B4. Unlike 2B4, rat NK cells express mRNA corresponding to both transmembrane (r2B4R-tm) and soluble (r2B4R-se) forms of r2B4R. r2B4R-tm contains an open reading frame encoding a polypeptide of 311 amino acid residues. The encoded protein has characteristics of type I transmembrane proteins with a 20-amino acid leader sequence, a 203-amino acid extracellular domain, a 23-amino acid transmembrane domain, and a 65-amino acid cytoplasmic domain. r2B4R-se encodes a protein of 205 amino acid residues without a putative transmembrane domain. Northern blot analysis and reverse transcriptase-PCR analysis revealed that both transmembrane and soluble forms of r2B4R are expressed in interleukin-2-activated NK cells.

Original languageEnglish (US)
Pages (from-to)306-313
Number of pages8
JournalImmunogenetics
Volume51
Issue number4-5
StatePublished - 2000
Externally publishedYes

Fingerprint

Natural Killer Cell Receptors
Molecular Cloning
Amino Acids
Natural Killer Cells
Natural Killer T-Cells
Northern Blotting
Immunoglobulins
Proteins
Reverse Transcriptase Polymerase Chain Reaction
Lectins
Open Reading Frames
Interleukin-2
Organism Cloning
Amino Acid Sequence
Complementary DNA
Ligands
Messenger RNA
Peptides
Genes

Keywords

  • 2B4
  • Immunoglobulin superfamily
  • Natural killer cells
  • Rat 2B4R

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

Kumaresan, P. R., Stepp, S. E., Bennett, M., Kumar, V., & Mathew, P. A. (2000). Molecular cloning of transmembrane and soluble forms of a novel rat natural killer cell receptor related to 2B4. Immunogenetics, 51(4-5), 306-313.

Molecular cloning of transmembrane and soluble forms of a novel rat natural killer cell receptor related to 2B4. / Kumaresan, Pappanaicken R.; Stepp, Susan E.; Bennett, Michael; Kumar, Vinay; Mathew, Porunelloor A.

In: Immunogenetics, Vol. 51, No. 4-5, 2000, p. 306-313.

Research output: Contribution to journalArticle

Kumaresan, PR, Stepp, SE, Bennett, M, Kumar, V & Mathew, PA 2000, 'Molecular cloning of transmembrane and soluble forms of a novel rat natural killer cell receptor related to 2B4', Immunogenetics, vol. 51, no. 4-5, pp. 306-313.
Kumaresan, Pappanaicken R. ; Stepp, Susan E. ; Bennett, Michael ; Kumar, Vinay ; Mathew, Porunelloor A. / Molecular cloning of transmembrane and soluble forms of a novel rat natural killer cell receptor related to 2B4. In: Immunogenetics. 2000 ; Vol. 51, No. 4-5. pp. 306-313.
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