Molecular cloning of the liver-specific rat F antigen

M. Eric Gershwin, R. L. Coppel, E. Bearer, M. G. Peterson, A. Sturgess, I. R. Mackay

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

F antigen is a 43-kDa widely conserved liver protein that has been intensively used in studies of immunogenicity and tolerance; two murine allotypes have been identified. Immunization of specific responder inbred strains with liver homogenates from the opposite allotype leads to precipitating antibody and cell-mediated immunity against F. The antibodies produced are autoantibodies as they react equally well with self. We have identified a cDNA clone from rat liver that reacts with alloantisera to F. The fused polypeptide produced by the clone was shown to correspond to F by several experiments. First, alloantisera to F antigen reacted with the cloned fused polypeptide, but not control recombinant clones. Second, mice immunized with the fused polypeptide generate an antibody response that reacts specifically with the 43-kDa protein of mouse liver homogenates and with highly purified F antigen. Finally, both anti-F allosera and sera from mice immunized with the fused polypeptide react with the same 43-kDa liver protein on two-dimensional immunoblots. The nucleotide and deduced amino acid sequence of the clone are presented and the sequence was found to have a significant homology with L28, an Escherichia coli ribosomal protein. The availability of recombinant F antigen will allow definitive questions to the addressed with respect to epitopes and specifically the identification of the T cell epitope which allows for autoimmune responses.

Original languageEnglish (US)
Pages (from-to)3828-3833
Number of pages6
JournalJournal of Immunology
Volume139
Issue number11
StatePublished - 1987

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Molecular Cloning
Clone Cells
Liver
Antigens
Peptides
Proteins
T-Lymphocyte Epitopes
Antibodies
Ribosomal Proteins
Escherichia coli Proteins
Autoimmunity
Cellular Immunity
Autoantibodies
Antibody Formation
Epitopes
Amino Acid Sequence
Immunization
Nucleotides
Complementary DNA
liver specific F antigen

ASJC Scopus subject areas

  • Immunology

Cite this

Gershwin, M. E., Coppel, R. L., Bearer, E., Peterson, M. G., Sturgess, A., & Mackay, I. R. (1987). Molecular cloning of the liver-specific rat F antigen. Journal of Immunology, 139(11), 3828-3833.

Molecular cloning of the liver-specific rat F antigen. / Gershwin, M. Eric; Coppel, R. L.; Bearer, E.; Peterson, M. G.; Sturgess, A.; Mackay, I. R.

In: Journal of Immunology, Vol. 139, No. 11, 1987, p. 3828-3833.

Research output: Contribution to journalArticle

Gershwin, ME, Coppel, RL, Bearer, E, Peterson, MG, Sturgess, A & Mackay, IR 1987, 'Molecular cloning of the liver-specific rat F antigen', Journal of Immunology, vol. 139, no. 11, pp. 3828-3833.
Gershwin ME, Coppel RL, Bearer E, Peterson MG, Sturgess A, Mackay IR. Molecular cloning of the liver-specific rat F antigen. Journal of Immunology. 1987;139(11):3828-3833.
Gershwin, M. Eric ; Coppel, R. L. ; Bearer, E. ; Peterson, M. G. ; Sturgess, A. ; Mackay, I. R. / Molecular cloning of the liver-specific rat F antigen. In: Journal of Immunology. 1987 ; Vol. 139, No. 11. pp. 3828-3833.
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