Abstract
Of the four major types of Ca channel described in vertebrate cells (designated T, L, N, and P), N-type Ca channels are unique in that they are found specifically in neurons, have been correlated with control of neurotransmitter release, and are blocked by ω-conotoxin, a neuropeptide toxin isolated from the marine snail Conus geographus. A set of overlapping cDNA clones were isolated and found to encode a Ca channel α-1 subunit, designated rbB-I. Polyclonal antiserum generated against a peptide from the rbB-I sequence selectively immunoprecipitates high-affinity 125I-labeled ω-conotoxin-binding sites from labeled rat forebrain membranes. PCR analysis shows that, like N-type Ca channels, expression of rbB-I is limited to the nervous system and neuronally derived cell lines. This brain Ca channel may mediate the ω-conotoxin-sensitive Ca influx required for neurotransmitter release at many synapses.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 5058-5062 |
| Number of pages | 5 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 89 |
| Issue number | 11 |
| State | Published - 1992 |
| Externally published | Yes |
Fingerprint
Keywords
- cDNA
- Dihydropyridine receptor
- Expression
- Immunoprecipitation
ASJC Scopus subject areas
- Genetics
- General
Cite this
Molecular cloning of the α-1 subunit of an ω-conotoxin-sensitive calcium channel. / Dubel, Stefan J.; Starr, Terry V B; Hell, Johannes W; Ahlijanian, Michael K.; Enyeart, John J.; Catterall, William A.; Snutch, Terry P.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, No. 11, 1992, p. 5058-5062.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular cloning of the α-1 subunit of an ω-conotoxin-sensitive calcium channel
AU - Dubel, Stefan J.
AU - Starr, Terry V B
AU - Hell, Johannes W
AU - Ahlijanian, Michael K.
AU - Enyeart, John J.
AU - Catterall, William A.
AU - Snutch, Terry P.
PY - 1992
Y1 - 1992
N2 - Of the four major types of Ca channel described in vertebrate cells (designated T, L, N, and P), N-type Ca channels are unique in that they are found specifically in neurons, have been correlated with control of neurotransmitter release, and are blocked by ω-conotoxin, a neuropeptide toxin isolated from the marine snail Conus geographus. A set of overlapping cDNA clones were isolated and found to encode a Ca channel α-1 subunit, designated rbB-I. Polyclonal antiserum generated against a peptide from the rbB-I sequence selectively immunoprecipitates high-affinity 125I-labeled ω-conotoxin-binding sites from labeled rat forebrain membranes. PCR analysis shows that, like N-type Ca channels, expression of rbB-I is limited to the nervous system and neuronally derived cell lines. This brain Ca channel may mediate the ω-conotoxin-sensitive Ca influx required for neurotransmitter release at many synapses.
AB - Of the four major types of Ca channel described in vertebrate cells (designated T, L, N, and P), N-type Ca channels are unique in that they are found specifically in neurons, have been correlated with control of neurotransmitter release, and are blocked by ω-conotoxin, a neuropeptide toxin isolated from the marine snail Conus geographus. A set of overlapping cDNA clones were isolated and found to encode a Ca channel α-1 subunit, designated rbB-I. Polyclonal antiserum generated against a peptide from the rbB-I sequence selectively immunoprecipitates high-affinity 125I-labeled ω-conotoxin-binding sites from labeled rat forebrain membranes. PCR analysis shows that, like N-type Ca channels, expression of rbB-I is limited to the nervous system and neuronally derived cell lines. This brain Ca channel may mediate the ω-conotoxin-sensitive Ca influx required for neurotransmitter release at many synapses.
KW - cDNA
KW - Dihydropyridine receptor
KW - Expression
KW - Immunoprecipitation
UR - http://www.scopus.com/inward/record.url?scp=0026750551&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026750551&partnerID=8YFLogxK
M3 - Article
C2 - 1317580
AN - SCOPUS:0026750551
VL - 89
SP - 5058
EP - 5062
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 11
ER -