TY - JOUR
T1 - Molecular cloning of the α-1 subunit of an ω-conotoxin-sensitive calcium channel
AU - Dubel, Stefan J.
AU - Starr, Terry V B
AU - Hell, Johannes W
AU - Ahlijanian, Michael K.
AU - Enyeart, John J.
AU - Catterall, William A.
AU - Snutch, Terry P.
PY - 1992
Y1 - 1992
N2 - Of the four major types of Ca channel described in vertebrate cells (designated T, L, N, and P), N-type Ca channels are unique in that they are found specifically in neurons, have been correlated with control of neurotransmitter release, and are blocked by ω-conotoxin, a neuropeptide toxin isolated from the marine snail Conus geographus. A set of overlapping cDNA clones were isolated and found to encode a Ca channel α-1 subunit, designated rbB-I. Polyclonal antiserum generated against a peptide from the rbB-I sequence selectively immunoprecipitates high-affinity 125I-labeled ω-conotoxin-binding sites from labeled rat forebrain membranes. PCR analysis shows that, like N-type Ca channels, expression of rbB-I is limited to the nervous system and neuronally derived cell lines. This brain Ca channel may mediate the ω-conotoxin-sensitive Ca influx required for neurotransmitter release at many synapses.
AB - Of the four major types of Ca channel described in vertebrate cells (designated T, L, N, and P), N-type Ca channels are unique in that they are found specifically in neurons, have been correlated with control of neurotransmitter release, and are blocked by ω-conotoxin, a neuropeptide toxin isolated from the marine snail Conus geographus. A set of overlapping cDNA clones were isolated and found to encode a Ca channel α-1 subunit, designated rbB-I. Polyclonal antiserum generated against a peptide from the rbB-I sequence selectively immunoprecipitates high-affinity 125I-labeled ω-conotoxin-binding sites from labeled rat forebrain membranes. PCR analysis shows that, like N-type Ca channels, expression of rbB-I is limited to the nervous system and neuronally derived cell lines. This brain Ca channel may mediate the ω-conotoxin-sensitive Ca influx required for neurotransmitter release at many synapses.
KW - cDNA
KW - Dihydropyridine receptor
KW - Expression
KW - Immunoprecipitation
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M3 - Article
C2 - 1317580
AN - SCOPUS:0026750551
VL - 89
SP - 5058
EP - 5062
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 11
ER -