Molecular cloning of the α-1 subunit of an ω-conotoxin-sensitive calcium channel

Stefan J. Dubel, Terry V B Starr, Johannes W Hell, Michael K. Ahlijanian, John J. Enyeart, William A. Catterall, Terry P. Snutch

Research output: Contribution to journalArticle

261 Citations (Scopus)

Abstract

Of the four major types of Ca channel described in vertebrate cells (designated T, L, N, and P), N-type Ca channels are unique in that they are found specifically in neurons, have been correlated with control of neurotransmitter release, and are blocked by ω-conotoxin, a neuropeptide toxin isolated from the marine snail Conus geographus. A set of overlapping cDNA clones were isolated and found to encode a Ca channel α-1 subunit, designated rbB-I. Polyclonal antiserum generated against a peptide from the rbB-I sequence selectively immunoprecipitates high-affinity 125I-labeled ω-conotoxin-binding sites from labeled rat forebrain membranes. PCR analysis shows that, like N-type Ca channels, expression of rbB-I is limited to the nervous system and neuronally derived cell lines. This brain Ca channel may mediate the ω-conotoxin-sensitive Ca influx required for neurotransmitter release at many synapses.

Original languageEnglish (US)
Pages (from-to)5058-5062
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number11
StatePublished - 1992
Externally publishedYes

Fingerprint

Conotoxins
Molecular Cloning
Calcium Channels
Neurotransmitter Agents
Marine Toxins
Conus Snail
Prosencephalon
Neuropeptides
Synapses
Nervous System
Vertebrates
Immune Sera
Complementary DNA
Clone Cells
Binding Sites
Neurons
Cell Line
Polymerase Chain Reaction
Peptides
Membranes

Keywords

  • cDNA
  • Dihydropyridine receptor
  • Expression
  • Immunoprecipitation

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Dubel, S. J., Starr, T. V. B., Hell, J. W., Ahlijanian, M. K., Enyeart, J. J., Catterall, W. A., & Snutch, T. P. (1992). Molecular cloning of the α-1 subunit of an ω-conotoxin-sensitive calcium channel. Proceedings of the National Academy of Sciences of the United States of America, 89(11), 5058-5062.

Molecular cloning of the α-1 subunit of an ω-conotoxin-sensitive calcium channel. / Dubel, Stefan J.; Starr, Terry V B; Hell, Johannes W; Ahlijanian, Michael K.; Enyeart, John J.; Catterall, William A.; Snutch, Terry P.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, No. 11, 1992, p. 5058-5062.

Research output: Contribution to journalArticle

Dubel, SJ, Starr, TVB, Hell, JW, Ahlijanian, MK, Enyeart, JJ, Catterall, WA & Snutch, TP 1992, 'Molecular cloning of the α-1 subunit of an ω-conotoxin-sensitive calcium channel', Proceedings of the National Academy of Sciences of the United States of America, vol. 89, no. 11, pp. 5058-5062.
Dubel, Stefan J. ; Starr, Terry V B ; Hell, Johannes W ; Ahlijanian, Michael K. ; Enyeart, John J. ; Catterall, William A. ; Snutch, Terry P. / Molecular cloning of the α-1 subunit of an ω-conotoxin-sensitive calcium channel. In: Proceedings of the National Academy of Sciences of the United States of America. 1992 ; Vol. 89, No. 11. pp. 5058-5062.
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