Abstract
Genome segments 2, 6, 8, and 9 of bluetongue virus (BTV) serotype 11, coding for P2, NS1, NS2, and P6, respectively, were cloned into pUC 8. Sizes of segment-2 and segment-6 clones indicated partial copies (55% and 80% of full length, respectively), whereas segment 8 and 9 clones represented full-length copies. Northern blot hybridizations of the clones to the 5 United States BTV prototypic serotypes (2, 10, 11, 13, and 17) revealed segment-2 clone to be serotype-specific to BTV-11, whereas segment 6, 8, and 9 clones were able to detect all serotypes to varying degrees. All clones failed to detect the related orbivirus, epizootic hemorrhagic disease virus.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1684-1689 |
| Number of pages | 6 |
| Journal | American Journal of Veterinary Research |
| Volume | 50 |
| Issue number | 10 |
| State | Published - Oct 1989 |
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Molecular cloning of serogroup- and serotype-specific genome segments from bluetongue virus serotype 11. / Dunn, S. J.; Oberst, R. D.; Stott, Jeffrey L; Osburn, Bennie.
In: American Journal of Veterinary Research, Vol. 50, No. 10, 10.1989, p. 1684-1689.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular cloning of serogroup- and serotype-specific genome segments from bluetongue virus serotype 11.
AU - Dunn, S. J.
AU - Oberst, R. D.
AU - Stott, Jeffrey L
AU - Osburn, Bennie
PY - 1989/10
Y1 - 1989/10
N2 - Genome segments 2, 6, 8, and 9 of bluetongue virus (BTV) serotype 11, coding for P2, NS1, NS2, and P6, respectively, were cloned into pUC 8. Sizes of segment-2 and segment-6 clones indicated partial copies (55% and 80% of full length, respectively), whereas segment 8 and 9 clones represented full-length copies. Northern blot hybridizations of the clones to the 5 United States BTV prototypic serotypes (2, 10, 11, 13, and 17) revealed segment-2 clone to be serotype-specific to BTV-11, whereas segment 6, 8, and 9 clones were able to detect all serotypes to varying degrees. All clones failed to detect the related orbivirus, epizootic hemorrhagic disease virus.
AB - Genome segments 2, 6, 8, and 9 of bluetongue virus (BTV) serotype 11, coding for P2, NS1, NS2, and P6, respectively, were cloned into pUC 8. Sizes of segment-2 and segment-6 clones indicated partial copies (55% and 80% of full length, respectively), whereas segment 8 and 9 clones represented full-length copies. Northern blot hybridizations of the clones to the 5 United States BTV prototypic serotypes (2, 10, 11, 13, and 17) revealed segment-2 clone to be serotype-specific to BTV-11, whereas segment 6, 8, and 9 clones were able to detect all serotypes to varying degrees. All clones failed to detect the related orbivirus, epizootic hemorrhagic disease virus.
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UR - http://www.scopus.com/inward/citedby.url?scp=0024746129&partnerID=8YFLogxK
M3 - Article
VL - 50
SP - 1684
EP - 1689
JO - American Journal of Veterinary Research
JF - American Journal of Veterinary Research
SN - 0002-9645
IS - 10
ER -