Molecular cloning of serogroup- and serotype-specific genome segments from bluetongue virus serotype 11.

S. J. Dunn, R. D. Oberst, Jeffrey L Stott, Bennie Osburn

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Genome segments 2, 6, 8, and 9 of bluetongue virus (BTV) serotype 11, coding for P2, NS1, NS2, and P6, respectively, were cloned into pUC 8. Sizes of segment-2 and segment-6 clones indicated partial copies (55% and 80% of full length, respectively), whereas segment 8 and 9 clones represented full-length copies. Northern blot hybridizations of the clones to the 5 United States BTV prototypic serotypes (2, 10, 11, 13, and 17) revealed segment-2 clone to be serotype-specific to BTV-11, whereas segment 6, 8, and 9 clones were able to detect all serotypes to varying degrees. All clones failed to detect the related orbivirus, epizootic hemorrhagic disease virus.

Original languageEnglish (US)
Pages (from-to)1684-1689
Number of pages6
JournalAmerican Journal of Veterinary Research
Volume50
Issue number10
StatePublished - Oct 1989

ASJC Scopus subject areas

  • veterinary(all)

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