Abstract
Genome segments 2, 6, 8, and 9 of bluetongue virus (BTV) serotype 11, coding for P2, NS1, NS2, and P6, respectively, were cloned into pUC 8. Sizes of segment-2 and segment-6 clones indicated partial copies (55% and 80% of full length, respectively), whereas segment 8 and 9 clones represented full-length copies. Northern blot hybridizations of the clones to the 5 United States BTV prototypic serotypes (2, 10, 11, 13, and 17) revealed segment-2 clone to be serotype-specific to BTV-11, whereas segment 6, 8, and 9 clones were able to detect all serotypes to varying degrees. All clones failed to detect the related orbivirus, epizootic hemorrhagic disease virus.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1684-1689 |
| Number of pages | 6 |
| Journal | American Journal of Veterinary Research |
| Volume | 50 |
| Issue number | 10 |
| State | Published - Oct 1989 |
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ASJC Scopus subject areas
- veterinary(all)
Cite this
Dunn, S. J., Oberst, R. D., Stott, J. L., & Osburn, B. (1989). Molecular cloning of serogroup- and serotype-specific genome segments from bluetongue virus serotype 11. American Journal of Veterinary Research, 50(10), 1684-1689.