Molecular cloning of nonsecreted endothelial cell-derived lipase isoforms

Tatsuro Ishida, Zhi Zheng, Helén L. Dichek, Huijian Wang, Ismael P Moreno, Eugene Yang, Ramendra K. Kundu, Said Talbi, Ken Ichi Hirata, Lawrence L. Leung, Thomas Quertermous

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

To expand our knowledge of factors involved in lipid metabolism in the blood vessel wall, we have cloned unique molecular isoforms of endothelial cell-derived lipase (EDL) (HGMW-approved symbol/LIPG). One isoform encoded a truncated protein (EDL2a) lacking the first 80 amino acid residues of the previously characterized EDL1a isoform, including the signal peptide. A similar second clone (EDL2b) was identified that lacked not only the first 80 amino acids, but also a 74-amino-acid region that encodes a portion of the lid domain. RT-PCR analysis confirmed expression of EDL2a/2b isoforms in several human tissues and cultured cells, including endothelial cells. Western blot and immunofluorescence studies using stable transfectants revealed that EDL2a and EDL2b were localized in the cytosol, while, EDL1a was secreted into the culture medium. Cell extracts of EDL2a/2b transfectants did not have triglyceride or phospholipase activity. Thus endothelial cells express three EDL isoforms, two of which remain intracellular and do not function as lipases.

Original languageEnglish (US)
Pages (from-to)24-33
Number of pages10
JournalGenomics
Volume83
Issue number1
DOIs
StatePublished - Jan 1 2004
Externally publishedYes

Fingerprint

Molecular Cloning
Lipase
Protein Isoforms
Endothelial Cells
Amino Acids
Phospholipases
Protein Sorting Signals
Cell Extracts
Lipid Metabolism
Cytosol
Fluorescent Antibody Technique
Blood Vessels
Culture Media
Cultured Cells
Triglycerides
Clone Cells
Western Blotting
Polymerase Chain Reaction
Proteins

Keywords

  • Endothelial cell
  • Endothelial cell-derived lipase
  • Lipoprotein
  • Microarray
  • Phospholipase

ASJC Scopus subject areas

  • Genetics

Cite this

Ishida, T., Zheng, Z., Dichek, H. L., Wang, H., Moreno, I. P., Yang, E., ... Quertermous, T. (2004). Molecular cloning of nonsecreted endothelial cell-derived lipase isoforms. Genomics, 83(1), 24-33. https://doi.org/10.1016/S0888-7543(03)00181-2

Molecular cloning of nonsecreted endothelial cell-derived lipase isoforms. / Ishida, Tatsuro; Zheng, Zhi; Dichek, Helén L.; Wang, Huijian; Moreno, Ismael P; Yang, Eugene; Kundu, Ramendra K.; Talbi, Said; Hirata, Ken Ichi; Leung, Lawrence L.; Quertermous, Thomas.

In: Genomics, Vol. 83, No. 1, 01.01.2004, p. 24-33.

Research output: Contribution to journalArticle

Ishida, T, Zheng, Z, Dichek, HL, Wang, H, Moreno, IP, Yang, E, Kundu, RK, Talbi, S, Hirata, KI, Leung, LL & Quertermous, T 2004, 'Molecular cloning of nonsecreted endothelial cell-derived lipase isoforms', Genomics, vol. 83, no. 1, pp. 24-33. https://doi.org/10.1016/S0888-7543(03)00181-2
Ishida, Tatsuro ; Zheng, Zhi ; Dichek, Helén L. ; Wang, Huijian ; Moreno, Ismael P ; Yang, Eugene ; Kundu, Ramendra K. ; Talbi, Said ; Hirata, Ken Ichi ; Leung, Lawrence L. ; Quertermous, Thomas. / Molecular cloning of nonsecreted endothelial cell-derived lipase isoforms. In: Genomics. 2004 ; Vol. 83, No. 1. pp. 24-33.
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