Abstract
Retroviruses cause a wide variety of diseases in avian and mammalian species. Human acquired immune deficiency syndrome (AIDS) leads to collapse of the immune system and death by a wide variety of opportunistic infections; unusual forms of cancer are associated with this syndrome. Retroviruses have been recovered from tissues of AIDS patients and from patients with related conditions. These similar newly-isolated viruses are lymphadenopathy-associated virus (LAV)1, human T-cell lymphotropic virus (HTLV-III) 2,3 and AIDS-associated retrovirus (ARV-2)4. We have identified a RNA genome of ∼9 kilobases (kb) in virions purified from the culture medium of a human T-cell tumour line infected with ARV-2. A cDNA probe made from viral RNA detected circular DNA molecules and proviral forms in infected cells. We prepared a library of infected cell DNA. Recombinant phage included those with a 9.5-kb proviral DNA and viral DNA permuted with respect to the single EcoRI site. Comparison of three ARV isolates from different AIDS patients revealed polymorphism of restriction endonuclease sites.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 760-763 |
| Number of pages | 4 |
| Journal | Nature |
| Volume | 312 |
| Issue number | 5996 |
| DOIs | |
| State | Published - 1984 |
| Externally published | Yes |
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Molecular cloning of AIDS-associated retrovirus. / Luciw, Paul A; Potter, Steven J.; Steimer, Kathelyn; Dina, Dino; Levy, Jay A.
In: Nature, Vol. 312, No. 5996, 1984, p. 760-763.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular cloning of AIDS-associated retrovirus
AU - Luciw, Paul A
AU - Potter, Steven J.
AU - Steimer, Kathelyn
AU - Dina, Dino
AU - Levy, Jay A.
PY - 1984
Y1 - 1984
N2 - Retroviruses cause a wide variety of diseases in avian and mammalian species. Human acquired immune deficiency syndrome (AIDS) leads to collapse of the immune system and death by a wide variety of opportunistic infections; unusual forms of cancer are associated with this syndrome. Retroviruses have been recovered from tissues of AIDS patients and from patients with related conditions. These similar newly-isolated viruses are lymphadenopathy-associated virus (LAV)1, human T-cell lymphotropic virus (HTLV-III) 2,3 and AIDS-associated retrovirus (ARV-2)4. We have identified a RNA genome of ∼9 kilobases (kb) in virions purified from the culture medium of a human T-cell tumour line infected with ARV-2. A cDNA probe made from viral RNA detected circular DNA molecules and proviral forms in infected cells. We prepared a library of infected cell DNA. Recombinant phage included those with a 9.5-kb proviral DNA and viral DNA permuted with respect to the single EcoRI site. Comparison of three ARV isolates from different AIDS patients revealed polymorphism of restriction endonuclease sites.
AB - Retroviruses cause a wide variety of diseases in avian and mammalian species. Human acquired immune deficiency syndrome (AIDS) leads to collapse of the immune system and death by a wide variety of opportunistic infections; unusual forms of cancer are associated with this syndrome. Retroviruses have been recovered from tissues of AIDS patients and from patients with related conditions. These similar newly-isolated viruses are lymphadenopathy-associated virus (LAV)1, human T-cell lymphotropic virus (HTLV-III) 2,3 and AIDS-associated retrovirus (ARV-2)4. We have identified a RNA genome of ∼9 kilobases (kb) in virions purified from the culture medium of a human T-cell tumour line infected with ARV-2. A cDNA probe made from viral RNA detected circular DNA molecules and proviral forms in infected cells. We prepared a library of infected cell DNA. Recombinant phage included those with a 9.5-kb proviral DNA and viral DNA permuted with respect to the single EcoRI site. Comparison of three ARV isolates from different AIDS patients revealed polymorphism of restriction endonuclease sites.
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UR - http://www.scopus.com/inward/citedby.url?scp=0021746228&partnerID=8YFLogxK
U2 - 10.1038/312760a0
DO - 10.1038/312760a0
M3 - Article
C2 - 6096718
AN - SCOPUS:0021746228
VL - 312
SP - 760
EP - 763
JO - Nature
JF - Nature
SN - 0028-0836
IS - 5996
ER -