Molecular cloning of AIDS-associated retrovirus

Paul A Luciw, Steven J. Potter, Kathelyn Steimer, Dino Dina, Jay A. Levy

Research output: Contribution to journalArticle

95 Scopus citations

Abstract

Retroviruses cause a wide variety of diseases in avian and mammalian species. Human acquired immune deficiency syndrome (AIDS) leads to collapse of the immune system and death by a wide variety of opportunistic infections; unusual forms of cancer are associated with this syndrome. Retroviruses have been recovered from tissues of AIDS patients and from patients with related conditions. These similar newly-isolated viruses are lymphadenopathy-associated virus (LAV)1, human T-cell lymphotropic virus (HTLV-III) 2,3 and AIDS-associated retrovirus (ARV-2)4. We have identified a RNA genome of ∼9 kilobases (kb) in virions purified from the culture medium of a human T-cell tumour line infected with ARV-2. A cDNA probe made from viral RNA detected circular DNA molecules and proviral forms in infected cells. We prepared a library of infected cell DNA. Recombinant phage included those with a 9.5-kb proviral DNA and viral DNA permuted with respect to the single EcoRI site. Comparison of three ARV isolates from different AIDS patients revealed polymorphism of restriction endonuclease sites.

Original languageEnglish (US)
Pages (from-to)760-763
Number of pages4
JournalNature
Volume312
Issue number5996
DOIs
StatePublished - 1984
Externally publishedYes

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Molecular cloning of AIDS-associated retrovirus'. Together they form a unique fingerprint.

  • Cite this

    Luciw, P. A., Potter, S. J., Steimer, K., Dina, D., & Levy, J. A. (1984). Molecular cloning of AIDS-associated retrovirus. Nature, 312(5996), 760-763. https://doi.org/10.1038/312760a0