Abstract
We isolated and sequenced caspase-10 cDNA and gene from Japanese flounder, Paralichthys olivaceus. The Japanese flounder (JF)-caspase-10 cDNA consisted of 2282 bp and encoded 495 amino acid residues. The characteristic death effector domains (DEDs) of caspases were observed in JF-caspase-10 as well as the three aspartic acid residues (D-186, -382 and -392), which are potential cleavage sites for the large and small subunit structures. The amino acid residue (His-325) and pentapeptide (QACQG), which are involved in catalytic activity, were absolutely conserved in Japanese flounder-caspase-10. JF-caspase-10 gene has a length of 6.6 kb and consists of 11 exons and 10 introns similar to that of human. The strong expression of JF-caspase-10 mRNA was detected in the gills, peripheral blood leukocytes, spleen and posterior kidney, while the weak expression was observed in the head kidney, heart, intestine, skin and stomach. The over-expression analysis of JF-caspase-10 in Japanese flounder cell line HINAE was shown to induce apoptosis 24 h post-transfection using TUNEL assay.
Original language | English (US) |
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Pages (from-to) | 1266-1274 |
Number of pages | 9 |
Journal | Fish and Shellfish Immunology |
Volume | 23 |
Issue number | 6 |
DOIs | |
State | Published - Dec 1 2007 |
Externally published | Yes |
Keywords
- Caspase-10
- Gene cloning
- Japanese flounder (Paralichthys olivaceus)
- Over-expression analysis
- RT-PCR
- TUNEL assay
ASJC Scopus subject areas
- Environmental Chemistry
- Aquatic Science