Abstract
We isolated and sequenced caspase-10 cDNA and gene from Japanese flounder, Paralichthys olivaceus. The Japanese flounder (JF)-caspase-10 cDNA consisted of 2282 bp and encoded 495 amino acid residues. The characteristic death effector domains (DEDs) of caspases were observed in JF-caspase-10 as well as the three aspartic acid residues (D-186, -382 and -392), which are potential cleavage sites for the large and small subunit structures. The amino acid residue (His-325) and pentapeptide (QACQG), which are involved in catalytic activity, were absolutely conserved in Japanese flounder-caspase-10. JF-caspase-10 gene has a length of 6.6 kb and consists of 11 exons and 10 introns similar to that of human. The strong expression of JF-caspase-10 mRNA was detected in the gills, peripheral blood leukocytes, spleen and posterior kidney, while the weak expression was observed in the head kidney, heart, intestine, skin and stomach. The over-expression analysis of JF-caspase-10 in Japanese flounder cell line HINAE was shown to induce apoptosis 24 h post-transfection using TUNEL assay.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1266-1274 |
| Number of pages | 9 |
| Journal | Fish and Shellfish Immunology |
| Volume | 23 |
| Issue number | 6 |
| DOIs | |
| State | Published - Dec 1 2007 |
| Externally published | Yes |
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Keywords
- Caspase-10
- Gene cloning
- Japanese flounder (Paralichthys olivaceus)
- Over-expression analysis
- RT-PCR
- TUNEL assay
ASJC Scopus subject areas
- Environmental Chemistry
- Aquatic Science
Cite this
Molecular cloning, expression, and functional analysis of caspase-10 from Japanese flounder Paralichthys olivaceus. / Kurobe, Tomofumi; Hirono, Ikuo; Kondo, Hidehiro; Yamashita, Michiaki; Aoki, Takashi.
In: Fish and Shellfish Immunology, Vol. 23, No. 6, 01.12.2007, p. 1266-1274.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular cloning, expression, and functional analysis of caspase-10 from Japanese flounder Paralichthys olivaceus
AU - Kurobe, Tomofumi
AU - Hirono, Ikuo
AU - Kondo, Hidehiro
AU - Yamashita, Michiaki
AU - Aoki, Takashi
PY - 2007/12/1
Y1 - 2007/12/1
N2 - We isolated and sequenced caspase-10 cDNA and gene from Japanese flounder, Paralichthys olivaceus. The Japanese flounder (JF)-caspase-10 cDNA consisted of 2282 bp and encoded 495 amino acid residues. The characteristic death effector domains (DEDs) of caspases were observed in JF-caspase-10 as well as the three aspartic acid residues (D-186, -382 and -392), which are potential cleavage sites for the large and small subunit structures. The amino acid residue (His-325) and pentapeptide (QACQG), which are involved in catalytic activity, were absolutely conserved in Japanese flounder-caspase-10. JF-caspase-10 gene has a length of 6.6 kb and consists of 11 exons and 10 introns similar to that of human. The strong expression of JF-caspase-10 mRNA was detected in the gills, peripheral blood leukocytes, spleen and posterior kidney, while the weak expression was observed in the head kidney, heart, intestine, skin and stomach. The over-expression analysis of JF-caspase-10 in Japanese flounder cell line HINAE was shown to induce apoptosis 24 h post-transfection using TUNEL assay.
AB - We isolated and sequenced caspase-10 cDNA and gene from Japanese flounder, Paralichthys olivaceus. The Japanese flounder (JF)-caspase-10 cDNA consisted of 2282 bp and encoded 495 amino acid residues. The characteristic death effector domains (DEDs) of caspases were observed in JF-caspase-10 as well as the three aspartic acid residues (D-186, -382 and -392), which are potential cleavage sites for the large and small subunit structures. The amino acid residue (His-325) and pentapeptide (QACQG), which are involved in catalytic activity, were absolutely conserved in Japanese flounder-caspase-10. JF-caspase-10 gene has a length of 6.6 kb and consists of 11 exons and 10 introns similar to that of human. The strong expression of JF-caspase-10 mRNA was detected in the gills, peripheral blood leukocytes, spleen and posterior kidney, while the weak expression was observed in the head kidney, heart, intestine, skin and stomach. The over-expression analysis of JF-caspase-10 in Japanese flounder cell line HINAE was shown to induce apoptosis 24 h post-transfection using TUNEL assay.
KW - Caspase-10
KW - Gene cloning
KW - Japanese flounder (Paralichthys olivaceus)
KW - Over-expression analysis
KW - RT-PCR
KW - TUNEL assay
UR - http://www.scopus.com/inward/record.url?scp=35649028361&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=35649028361&partnerID=8YFLogxK
U2 - 10.1016/j.fsi.2007.07.001
DO - 10.1016/j.fsi.2007.07.001
M3 - Article
VL - 23
SP - 1266
EP - 1274
JO - Fish and Shellfish Immunology
JF - Fish and Shellfish Immunology
SN - 1050-4648
IS - 6
ER -